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Citation: WU Hui Ling, LI Wen-You, HE Xi-Wen, LIANG Hong. Spectral Studies on Interactions of Quinaldine Blue with Nucleic Acids and Its Analytical Application[J]. Chinese Journal of Applied Chemistry, ;2002, 19(7): 672-676. shu

Spectral Studies on Interactions of Quinaldine Blue with Nucleic Acids and Its Analytical Application

1.
a Department of Chemistry, Nankai University, Tianjin 300071;
2.
b Department of Chemistry, Guangxi Normal University

Corresponding author: HE Xi-Wen,
Received Date: 30 November 2001
Available Online: 1 March 2002
Fund Project: 国家自然科学基金(29975014) (29975014)

In an aqueous solution of pH=5.0~8.2, H aggregates of Quinaldine Blue(QB) are formed with the nucleic acid molecules. QB showed a decrease in absorbance at peak wavelength of 598 nm or 548 nm on binding to nucleic acids. A new assay method for nucleic acids was thus developed based on clear decrease of QB absorbance at 598 nm. The calibration curves are linear in the range of 8.0×10^-8~2.0×10^-6g/mL for ctDNA, 1.0×10^-7~2.0×10^-6g/mL for yRNA, respectively. The reliabilities of five determinations of 1.0 μg/mL nucleic acids were 3.4% for ctDNA and 3.6% for yRNA, respectively. The method was featured by its rapidity, simplicity, sensitivity and good selectivity. It has been used to determine both synthetic and real samples with satisfactory results.
- nucleic acid,
- quinaldine blue,
- outside stacking binding,
- spectrophotometry
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