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Citation: LUO Yang-He, JIANG Zhi-Liang, YUAN Wei-En, PAN Hong-Cheng, TANG Guo-Shun. Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System[J]. Chinese Journal of Applied Chemistry, ;2003, 20(9): 833-836. shu

Fluorescence Quenching of HSA-Silicotungstic Heteropolyacid Associated Nanoparticle System

1.
Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004

Corresponding author: JIANG Zhi-Liang,
Received Date: 18 December 2002
Available Online: 12 May 2003
Fund Project: 广西自然科学基金资助项目(014403) (014403)

In a Tris buffer solution(pH=7.40), there are an absorption peak at 260 nm for silicotungstic heteropolyacid(SiW) system and independently a fluorescence peak at 350 nm for human serum albumin(HSA) system. When both HSA and SiW solutions are mixed, ion association nanoparticles in size of 50 nm are formed. As a result Rayleigh scattering peak at 470 nm was enhanced considerably and the fluorescence peak at 350 nm was quenched. The Rayleigh scattering spetra and TEM observation revealed that the formation of HSA-SiW nanoparticles and interface formation are main factors that causes the fluorescence quenching and Rayleigh scattering enhancement.
- silicotungstic heteropolyacid,
- HSA,
- association nanoparticle,
- fluorescence quenching,
- Rayleigh scattering
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