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Citation: DAI Shi-Kun, CHI Yan-Hua, WANG Xiao-Chuan, YU Kun, LIU Ning. Determination of Cytidine-5'-phosphate by Spectrophotometry[J]. Chinese Journal of Applied Chemistry, ;2004, 21(4): 422-424. shu

Determination of Cytidine-5'-phosphate by Spectrophotometry

1.
a Department of Chemistry and Biological Engineers, School of Materials Science and Engineering, Southwest University of Science and Technology, Mianyang 621002;
2.
b Institute of Chemical Material, CAEP, Mianyang

Corresponding author: CHI Yan-Hua,
Received Date: 4 June 2003
Available Online: 20 August 2003
Fund Project: 教育部科学技术研究重点项目(02016) (02016)四川省教育厅自然科学基金(2002C04) (2002C04)

The binding reaction of cytidine 5'-phosphate(5'-CMP) with Alizarin Red S(ARS) was studied in the buffer solution(pH=4.56) as functions of temperature, time, ionic strength and interference of inorganic substance, biological substance and surfactant by UV-Vis spectrophotometry. It was found that the bathochromic shift of the maximum absorption peak of the complex was 90nm. The apparemt molar absorptivity was ε=3.80×10³ L/(mol·cm), the binding number was n=13, and the linear range of reaction was 0~14 mg/L.
- cytidine phosphate,
- alizarin Red S,
- UV-Vis spectrophotometry
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