Home

Citation: WEI Yong-Feng, MA Dong-Mei, LI Xiao-Hua. Chitosan-immobilized Urease and Analysis of Urea in Waste Water[J]. Chinese Journal of Applied Chemistry, ;2004, 21(4): 357-360. shu

Chitosan-immobilized Urease and Analysis of Urea in Waste Water

1.
Department of Chemistry, Northwest University, Xi'an 710069

Corresponding author: WEI Yong-Feng,
Received Date: 6 June 2003
Available Online: 5 September 2003
Fund Project: 陕西省自然科学基金(01JK073) (01JK073)

The porous chitosan microspheres were prepared. Their activation and immobilization with urease were investigated. It is found that the immobilized urease exihibited storage stability better than the free urease, but with lower activity. The chitosan-immobilized uease was applied as a bioreactor in the determination of urea in waste water by flow injection analysis. The linear range of urea concentration was 1.0×10^-5~5.0×10^-3 g/mL with the mean recovery being (100.5±3.2)%, detection limit was 1.0×10^-5g/mL
- urease,
- chitosan,
- immobilization,
- bioreactor,
- urea determination
1. [1]
  Dong-Bing Cheng , Junxin Duan , Haiyu Gao . Experimental Teaching Design on Chitosan Extraction and Preparation of Antibacterial Gel. University Chemistry, 2024, 39(2): 330-339. doi: 10.3866/PKU.DXHX202308053
2. [2]
  Huairou Zhu , Qiaoyi Cen , Dan Yan , Wei Yi , Guosheng Chen , Siming Huang , Gangfeng Ouyang . 基于仿生矿化酶固定纳米探针对血清葡萄糖定量检测的实验新探索. University Chemistry, 2026, 41(5): 330-339. doi: 10.12461/PKU.DXHX202511041
3. [3]
  Yueming Zhou , Xin Qiu , Xin Zhou , Xiaotian Wan , Mofan Zhang , Feng Li , Xinxin Shao , Peng Ding , Xizhen Liang . Intelligent Visualization of Potassium Dichromate Reflux Method for Determination of Chemical Oxygen Demand. University Chemistry, 2026, 41(1): 85-94. doi: 10.12461/PKU.DXHX202506021
4. [4]
  Kai Ye , Lizhong Zhang , Mingyu Zhang , Qinxiong Wu , Kui Wang , Qi Wang . Digital Experiment for the Determination of Liquid Saturated Vapor Pressure. University Chemistry, 2026, 41(1): 227-243. doi: 10.12461/PKU.DXHX202503107
5. [5]
  Haobin Zhou , Shuai Du , Xuesong Guo , Yue Wang , Qin Zhong , Tong Wang , Yuzhi Wang , Shuangyan Huan , Hailong Yan , Kun Li . 阳极溶出伏安法测定水中微量镉的无汞化改进. University Chemistry, 2026, 41(5): 275-284. doi: 10.12461/PKU.DXHX202511142
6. [6]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
7. [7]
  Ziyu Bai , Jiaxin Zhou , Sisi Zhao , Siyu Teng , Xinxin Li , Xiting Wang , Yuwei Dong , Zhen Zhao . Delving into the Captivating Color Transformation of Cobalt: Digital Empowerment of Intelligent Visual Robot Monitoring System for Measuring Thermodynamic Parameters. University Chemistry, 2026, 41(1): 122-132. doi: 10.12461/PKU.DXHX202505103
8. [8]
  Chunfang Zhang , Cuimiao Zhang , Shuai Liu , Yaduo Zheng , Lin Yang , Kehan Jiang , Mingtao Run . Refined Experiment for Magnetic Susceptibility Determination: A Digital Empowered Experiment Based on WeChat Mini Program and Animated Video. University Chemistry, 2026, 41(1): 244-252. doi: 10.12461/PKU.DXHX202505056
9. [9]
  Jiaqi Chen , Liang Chen , Xiaocui Wei , Yankai Wang , Yahui Chang , Xinghao Ji , Haoyu Yang , Yue Sun , Yawen Wang , Xiufeng Shi , Xu Wu . Digital Empowerment for Foundational Excellence: A Digitally Enhanced Coordination Titration Experiment of Heating Pack Component Analysis. University Chemistry, 2026, 41(1): 382-393. doi: 10.12461/PKU.DXHX202506008
10. [10]
  Qiuting Zhang , Fan Wu , Jin Liu , Zian Lin . Chromatographic Stationary Phase and Chiral Separation Using Frame Materials. University Chemistry, 2025, 40(4): 291-298. doi: 10.12461/PKU.DXHX202405174
11. [11]
  Sihan Wang , Chenxi Yu , Shuzhang Ran , Jiawei Chen , Shoutong Rao , Xinyi Liang , Ruiqi Dong , Guixiang Zeng , Guoqiang Wang , Jing Ma . 铁配合物合成与性质探究的数智化改进——大学化学实验中“磺基水杨酸合铁配合物的组成与稳定常数的测定”的创新设计. University Chemistry, 2026, 41(5): 36-49. doi: 10.12461/PKU.DXHX202511137
12. [12]
  Xin Feng , Kexin Guo , Chunguang Jia , Bowen Liu , Suqin Ci , Junxiang Chen , Zhenhai Wen . Hydrogen Generation Coupling with High-Selectivity Electrocatalytic Glycerol Valorization into Formate in an Acid-Alkali Dual-Electrolyte Flow Electrolyzer. Acta Physico-Chimica Sinica, 2024, 40(5): 2303050-0. doi: 10.3866/PKU.WHXB202303050
13. [13]
  Yukai SHEN , Zhaochao YAN , Yangjun ZHOU , Mei HUANG . Nickel foam-supported NiFeP/NiFcDCA heterojunction electrocatalyst for efficient urea oxidation reaction. Chinese Journal of Inorganic Chemistry, 2026, 42(2): 237-246. doi: 10.11862/CJIC.20250257
14. [14]
  Siming Bian , Sijie Luo , Junjie Ou . Application of van Deemter Equation in Instrumental Analysis Teaching: A New Type of Core-Shell Stationary Phase. University Chemistry, 2025, 40(3): 381-386. doi: 10.12461/PKU.DXHX202406087
15. [15]
  Hongpeng He , Mengmeng Zhang , Mengjiao Hao , Wei Du , Haibing Xia . Synthesis of Different Aspect-Ratios of Fixed Width Gold Nanorods. Acta Physico-Chimica Sinica, 2024, 40(5): 2304043-0. doi: 10.3866/PKU.WHXB202304043
16. [16]
  Xuanzhu Huo , Yixi Liu , Qiyu Wu , Zhiqiang Dong , Chanzi Ruan , Yanping Ren . Integrated Experiment of “Electrolytic Preparation of Cu₂O and Gasometric Determination of Avogadro’s Constant: Implementation, Results, and Discussion: A Micro-Experiment Recommended for Freshmen in Higher Education at Various Levels Across the Nation. University Chemistry, 2024, 39(3): 302-307. doi: 10.3866/PKU.DXHX202308095
17. [17]
  Mingjie Lei , Wenting Hu , Kexin Lin , Xiujuan Sun , Haoshen Zhang , Ye Qian , Tongyue Kang , Xiulin Wu , Hailong Liao , Yuan Pan , Yuwei Zhang , Diye Wei , Ping Gao . Accelerating the reconstruction of NiSe₂ by Co/Mn/Mo doping for enhanced urea electrolysis. Acta Physico-Chimica Sinica, 2025, 41(8): 100083-0. doi: 10.1016/j.actphy.2025.100083
18. [18]
  Qing Li , Guangxun Zhang , Yuxia Xu , Yangyang Sun , Huan Pang . P-Regulated Hierarchical Structure Ni₂P Assemblies toward Efficient Electrochemical Urea Oxidation. Acta Physico-Chimica Sinica, 2024, 40(9): 2308045-0. doi: 10.3866/PKU.WHXB202308045
19. [19]
  Hao GUO , Tong WEI , Qingqing SHEN , Anqi HONG , Zeting DENG , Zheng FANG , Jichao SHI , Renhong LI . Electrocatalytic decoupling of urea solution for hydrogen production by nickel foam-supported Co₉S₈/Ni₃S₂ heterojunction. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2141-2154. doi: 10.11862/CJIC.20240085
20. [20]
  Wenjuan Tan , Yong Ye , Xiujuan Sun , Bei Liu , Jiajia Zhou , Hailong Liao , Xiulin Wu , Rui Ding , Enhui Liu , Ping Gao . Building P-Poor Ni₂P and P-Rich CoP₃ Heterojunction Structure with Cation Vacancy for Enhanced Electrocatalytic Hydrazine and Urea Oxidation. Acta Physico-Chimica Sinica, 2024, 40(6): 2306054-0. doi: 10.3866/PKU.WHXB202306054

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(640)
HTML views(44)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Address：Zhongguancun North First Street 2,100190 Beijing, PR China Tel: +86-010-82449177-888

DownLoad: Full-Size Img PowerPoint

Return