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Citation: LIU Ping, LI Yu-Guang, CHU Yi-Ming, MA Jie, XUE Ying, WU Guo-Hua, LIU Meng-You, JIA Jing-Jin. Determination of Trace Penicillin in Milk by Home-made Enzyme Electrode[J]. Chinese Journal of Applied Chemistry, ;2004, 21(8): 832-834. shu

Determination of Trace Penicillin in Milk by Home-made Enzyme Electrode

LIU Ping ^a ,
LI Yu-Guang ^a ,
CHU Yi-Ming ^a ,
MA Jie ^a,, ,
XUE Ying ^b ,
WU Guo-Hua ^b ,
LIU Meng-You ^a ,
JIA Jing-Jin ^a

1.
a Department of Chemistry, Capital Normal University, Beijing 100037;
2.
b Beijing Center for Disease Prevention and Control, Beijing

Corresponding author: MA Jie,
Received Date: 10 October 2003
Available Online: 11 April 2004
Fund Project: 北京市教委资助项目(2002KJ094) (2002KJ094)

Penicillinase was immobilized by glutaraldehyde-bovine serum albumin and sodium alginate was used for the construction of enzyme biosensors in determination of trace penicillin in milk. Penicillinase activity was determined by potentionmetric method. At low concentrations, the response time of the electrode modified by the method of crossing-link was 30~60 s, and the electrode showed high sensitivity. Linear detection range from 1.40×10^-3 to 3.36×10^-2 mmol/L in PBS and from 5.61×10^-2 to 3.36×10^-1 mmol/L in milk were achieved. The electrode can be used for at least 7 d with perfect reproducibility during the first 4 d. The life of the electrode was 30 d.
- electrochemical biosensor,
- enzyme electrode,
- trace penicillin
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