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Citation: XIANG Hai-Yan, ZHOU Chun-Shan, ZHONG Shi-An, LEI Qi-Fu. Synthesis of Resveratrol Imprinted Polymer and Its Application in Separation of Active Ingredent in Polygonum cuspidatum Sieb.et Zucc.Extracts[J]. Chinese Journal of Applied Chemistry, ;2005, 22(7): 739-743. shu

Synthesis of Resveratrol Imprinted Polymer and Its Application in Separation of Active Ingredent in Polygonum cuspidatum Sieb.et Zucc.Extracts

1.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083

Corresponding author: ZHOU Chun-Shan,
Received Date: 25 September 2004
Available Online: 16 January 2005
Fund Project: 中国博士后科学基金(2004035085) (2004035085)湖南省自然科学基金(04JJ3080)资助课题 (04JJ3080)

Resveratrol,a promising anti-cancer agent,is an important active ingredent in Polygonum cuspidatum Sieb.et Zucc.A molecular imprinted polymer was prepared by virtue of molecular imprinting technique,with trans-resveratrol as the template molecular,acrylamide as the functional monomer,EGDMA as the cross-linking agent.The molecular imprinted polymer was investigated with the aid of equilibrium binding experiments to evaluate the molecular recognition and binding characteristics of the trans-resveratrol molecularly imprinted polymer.The scatchard analysis showed that two kinds of binding sites were produced in the polymer matrix and their dissociation constants were calculated to be 9.9×10^-5 mol/L,1.7×10^-3 mol/L,respec-tively.The results show that compared with non-imprinted polymer,the imprinted polymer exhibits a much higher affinity for trans-resveratrol among the tested compounds Res,Pol and BPA.Also,the imprinted polymer was applied to separate resveratrol from Polygonum cuspidatum Sieb.et Zucc.extracts and good results were obtained.
- molecularly imprinted polymer,
- resveratrol,
- Polygonum cuspidatum Sieb.et Zucc.,
- binding characteristics,
- substrate selectivity
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