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Citation: JIANG De-Sheng, LONG Sheng-Ya, XIAO Hai-Yan, ZHOU Ju-Ying, HUANG Jun. Preparation of Magnetic Chitosan Microspheres and Its Application to Immobilization of Laccase[J]. Chinese Journal of Applied Chemistry, ;2005, 22(10): 1122-1126. shu

Preparation of Magnetic Chitosan Microspheres and Its Application to Immobilization of Laccase

1.
Fiber Optic Sensing Technology Research Center, Wuhan University of Technology, Key Laboratory of Fiber Optic Sensing Technology and Information Processing, Ministry of Education, Wuhan 430070

Corresponding author: HUANG Jun,
Received Date: 6 November 2004
Available Online: 25 May 2005

Magnetic chitosan microspheres were prepared by using glutaradehyde as the cross-linker.The morphology and structure of the microspheres were observed and analyzed.The results show that the magnetic chitosan microspheres were well-shaped spherical with smooth surface,and the average particle size was 0.9 μm with a narrow size distribution.The laccase was immobilized on the magnetic chitosan microspheres.The optimal temperatures for the immobilized enzyme were 10℃ and 55℃,5℃ lower than that of the free enzyme,respectively.The optimal pH for the immobilized enzyme was 3.02,the same as that for free enzyme.After the immobilized enzyme was kept at 60℃ for 210 min,it lost 26% of the initial enzyme activity,while the free enzyme lost 80.6% under the same conditions.After 10 cycles of operation,the immobilized enzyme remained over 80% of its initial activity.Therefore,the catalytic efficiency and storage stability of the laccase were greatly improved after its immobilization on the magnetic chitosan microspheres.
- chitosan,
- immobilization,
- laccase,
- enzyme activity
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