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Citation: LIANG Ru-Ping, RONG Lian-Qing, SU Ying-Yi, ZOU Xiao-Yong, CAI Pei-Xiang. Renewable Immunosensor for Determination of AFP Based on Sol-gel Technology[J]. Chinese Journal of Applied Chemistry, ;2005, 22(11): 1192-1196. shu

Renewable Immunosensor for Determination of AFP Based on Sol-gel Technology

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a Department of Chemistry, Nanchang University, Nanchang;
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b School of Chemistry and Chemical Engineering, Zhongshan University, Guangzhou 510275;
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c Department of Chemical Engineering, Pingxiang College, Pingxiang

Corresponding author: CAI Pei-Xiang,
Received Date: 22 December 2004
Available Online: 14 April 2005
Fund Project: 广东省自然科学基金(011105) (011105)江西省教育厅科技计划(赣教技字[2005]241号)资助项目 (赣教技字[2005]241号)

A novel immunosensor for the determination of alpha fetoprotein(AFP) based on sol-gel technique was constructed by dispersing graphite and alpha fetoprotein in tetraethylorthosilicate(TEOS) derived sol-gel.With the increase of specific immuno-reaction complex of antibody and antigen,the membrane potential of the immunosensor changes.The membrane potential value is correlative with the antigen concentration in solution.According to this relationship,AFP can be detected quantificationally,the linearity range for determining AFP is 5.0×10^-9~5.0×10^-8g/mL with a correlation coefficient of 0.999 4.Other antigens such as carcino emtynic antigen(CEA),ferritin and hepatitis B surface antigen(HBsAg) do not interfere with the determination.The dependences of the potential response on immobilization method,incubation time,sensitivity and reproducibility were studied.The immunosensor was found to be stable for about 30 days when it was stored in dry state at 4℃.A satisfactory determination of AFP in serum samples can be obtained by this immunosensor.
- sol-gel,
- alpha fetoprotein,
- immunosensor,
- regeneration
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