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Citation: PAN Hui-Ying, GE Feng-Yan, CHEN Li-Gong. Synthesis,Separation and Characterization of Aminofluorescein[J]. Chinese Journal of Applied Chemistry, ;2006, 23(2): 193-197. shu

Synthesis,Separation and Characterization of Aminofluorescein

1.
College of Pharmaceuticals and Biotechnology, Tianjin University, Tianjin 300072

Corresponding author: CHEN Li-Gong,
Received Date: 16 April 2005
Available Online: 2 August 2005

The synthesis of aminofluorescein,an important fluorescein probe,was studied systematically.A nitrofluorescein compound was obtained from 4-nitrophthalic acid and resoreinol in the presence of zinc chloride by melting reaction with a yield of 85.0%.Reduction of the nitrofluorescein compound with Na₂S/NaHS,Na₂S₂,SnCl₂,or H₂/Raney nickel yielded aminofluorescein compounds.5-Aminofluorescein and 6-aminofluorescein were isolated by recrystallization of the obtained mixture in 6% hydrochloric acid with 39.6% and 14.5% yields respectively.The fluorescence intensity of the product,λ_EX and λ_EM,were determined with a fluorometer.The purities of the products were identified with HPLC,and the structures of the products were charac-terized by ¹H NMR,¹³C NMR,IR and MS.
- aminofluorescein,
- nitrofluorescein,
- reduction,
- separation
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