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Citation: LÜ Bin, JIANG Ming, SHI Dan, ZHANG Jiang-Hua, MEI Su-Rong, ZHU Ye-Xiang, HAO Qiao-Ling, ZHOU Yi-Kai. Syntheses of Cholesterol-MIPs and Their Absorption Properties and Applications[J]. Chinese Journal of Applied Chemistry, ;2006, 23(4): 353-356. shu

Syntheses of Cholesterol-MIPs and Their Absorption Properties and Applications

1.
Tongji Public Health College, Huazhong University of Science and Technology, Wuhan 430030

Corresponding author: LÜ Bin,
Received Date: 20 May 2005
Available Online: 3 July 2005
Fund Project: 国家自然科学基金(30200061) (30200061)湖北省卫生厅(JX1B031)资助项目 (JX1B031)

Non-covalent molecular imprinted polymers(MIP) of cholesterol were prepared from its reaction with methacrylic acid(MAA),ethylene glycol dimethacrylate(EGDMA) and azobisisobutyronitrile(AIBN) via thermal and UV polymerization.The binding capability and selectivity of the different polymers for cholesterol were studied.The polymers were used as immunosorbents for the selective enrichment of cholesterol in different biological samples(serum,milk and yolk) under optimal loading,washing and eluting conditions.Results show that MIPs can selectively recognize and separate cholesterol in all three biological samples.The rate of recovery of cholesterol from serum was the highest,while that from yolk was the lowest.Co MPared with C18 SPE,the UV initiated MIPs show higher recovery than C18 for cholesterol in the sample.
- molecular imprinted polymer,
- solid phase extraction,
- cholesterol,
- biological sample,
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