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Citation: GAN Zheng-Yan, WUMANJIANG Eli, JIA Dian-Zeng, Xamxi Kamar. Lipase Catalyzed Hydrolysis of Safflower Oil in a Solvent-free Medium[J]. Chinese Journal of Applied Chemistry, ;2006, 23(8): 902-906. shu

Lipase Catalyzed Hydrolysis of Safflower Oil in a Solvent-free Medium

1.
a College of Chemical Engineering, Xinjiang University, Urumqi;
2.
b Xinjiang Research Institute of Physical and Chemical Technology, Chinese Academy of Sciences, Urumqi 830011

Received Date: 3 October 2005
Available Online: 15 January 2006

Enzymatic hydrolysis of safflower oil catalyzed by a lipase from Aspergillus niger was investigated in a solvent-free system,and the effect of reaction time on the hydrolysis was studied.In addition,the selectivity of the lipase was also examined by testing the monoglycerides content in the ester and the iodine values(Ⅳ) of the fatty acid and the ester.The experiment showed that the optimum conditions for an 1 h reaction were m(H₂O):m(oil)=0.5:1,m(lipase):m(oil)=5:100,35℃,pH=7.0 and 0.2% CaCl₂ and the optimum conditions for an 24 h reaction were m(H₂O):m(oil)=1:1,m(lipase):m(oil)=1:100,26℃,pH=7.6 and no CaCl₂.The lipase has no selectivity on either the fatty acid or the site of the ester bond on the acylglycerol.
- hydrolysis,
- safflower oil,
- solvent-free medium,
- lipase
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