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Citation: ZHONG Chong-Lin, ZHAO Wei-Min, YANG Mei-Lin, LIU Shi-Yue, XU Jia-Jie. Determination of The Rhynchophylline Contents in Five Kinds of Uncaria by RP-HPLC[J]. Chinese Journal of Applied Chemistry, ;2006, 23(9): 1061-1062. shu

Determination of The Rhynchophylline Contents in Five Kinds of Uncaria by RP-HPLC

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a Jilin Provincial Institute of Traditional Chinese Medicine and Materia Medica, Changchun 130021;
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b The Affiliated Hospital of Changchun University of TCM, Changchun;
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c Jilin Aodong Yanbian Pharmaceutical Co., Ltd., Dunhua

Corresponding author: ZHONG Chong-Lin,
Received Date: 10 November 2005
Available Online: 10 March 2006
Fund Project: 国家中医药管理局资助项目(国中医药科00-01K(ZL)) (国中医药科00-01K(ZL))

An RP-HPLC method was used to determine the rhynchophylline content in five kinds of uncaria.The mobile phase was a mixture of V(CH₃OH):V(0.02%(volume fraction) C₂H₅)₃N)=70:30, detection wavelength 250 nm,and the average recovery of rhynchophylline was 98.98%,RSD=1.31%(n=5).The methods is simple and accurate.
- uncaria,
- rhynchophylline,
- HPLC
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