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Citation: PAN Hua, ZHANG Jian, ZHANG Gao-Yong, SUN Yan-Qing. Phase Diagram on Interaction Between Polyamine and DNA[J]. Chinese Journal of Applied Chemistry, ;2007, 24(11): 1250-1254. shu

Phase Diagram on Interaction Between Polyamine and DNA

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a State Center for Surfactant Research and Engineering, China Research Institute of Daily Chemical Industry, Taiyuan;
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b Genencor(Wuxi) Bio-Products Co. Ltd, Shanghai 200051;
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c Satellite R & D, Asia Pacific Avon Manufacturins(Guangzhou) LTD, Guangzhou

Corresponding author: ZHANG Jian,
Received Date: 28 December 2006
Available Online: 28 April 2007
Fund Project: 国家自然科学基金(20233010)重点资助项目 (20233010)

The interaction between DNA and polyamine(PA) with different valency was studied via phase diagram. The influence of NaCl on the interaction between spermine(SPM) and DNA was also studied primarily. The result shows that there was electrostatic attraction between SPM and DNA, and interaction between SPM and DNA base pair as well. The SPM-DNA syetem began to show turbidity at a low SPM concentration(0.025 mmol/L), while the turbidities of spermidine(SPD)-DNA and guanidine hydrochloride(GD)-DNA systems didn't change much. With the increasing of polyamine, the A₂₆₀ of the SPD-DNA system reduced while the A₂₆₀ of the GD-DNA system didn't change much when the concentration of polyamine was 10 mmol/L, showing the interaction between SPD and DNA was stronger than that of GD-DNA system. So the higher the valeny of the polyamine, the stronger the interaction of the system. In aqueous solution, interactions between DNA and SPD or GD were weaker, and no phase separation was observed. After the addition of NaCl, the interaction between SPM and DNA will be baffled because of electrostatic-screening effect. The higher the NaCl concentration, the weaker the interaction and the worse the linearity of the phase curve of the system.
- polyamine,
- DNA,
- phase diagram,
- interaction
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