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Citation: WANG Xiang-Hong, YANG Ji-Dong, LIU Shao-Pu, LIU Zhong-Fang. Resonance Rayleigh Scattering Method for Determination of Sodium Aescinate by Means of Night Blue[J]. Chinese Journal of Applied Chemistry, ;2008, 25(6): 656-660. shu

Resonance Rayleigh Scattering Method for Determination of Sodium Aescinate by Means of Night Blue

1.
a College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715;
2.
b Department of Chemistry, Changjiang Normal University, Chongqing

Corresponding author: LIU Shao-Pu,
Received Date: 23 June 2007
Available Online: 13 September 2007
Fund Project: 国家自然科学基金资助项目(20475045) (20475045)

In a BR buffer medium of pH 4.5~5.5,sodium aescinate can be bound to Night blue dye to form a binding product of 1:1,which results in great enhancement of RRS and a new RRS spectrum appears.The maximum RRS peak was located at 416 nm and the intensity of RRS was directly proportional to the concentration of SA in a range of 0.025~20.0×10^-6 g/mL for an NB-SA system.The method had high sensitivity and the detecting limit of SA (3σ) was 7.5×10^-9 g/mL for an NB-SA system.The influences of some coexisiting substances were investigated.The RRS method was applied to the determination of trace amounts of sodium aescinate in injection samples,tablet samples and urine samples with satisfactory results.
- sodium aescinate,
- night blue,
- Resonance Rayleigh scattering
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