Citation: Yang Zhigang, Xiong Jia, Zhang Wei, Li Wen, Pan Wenhui, Zhang Jianguo, Gu Zhenyu, Huang Meina, Qu Junle. A Reversibly Intramolecular Cyclization Cy5 Optical Probe for Stochastic Optical Reconstruction Microscopy in Live Cell Mitochondria[J]. Acta Chimica Sinica, ;2020, 78(2): 130-139. doi: 10.6023/A19100374 shu

A Reversibly Intramolecular Cyclization Cy5 Optical Probe for Stochastic Optical Reconstruction Microscopy in Live Cell Mitochondria

Center of Biomedical Photonics, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060

Corresponding author: Xiong Jia, Qu Junle, zhgyang@szu.edu.cn

^†

Received Date: 19 October 2019
Available Online: 19 February 2019
Fund Project: the National Natural Science Foundation of China 61875131Shenzhen Basic Research Project JCYJ20180305125549234the National Natural Science Foundation of China 61525503Project supported by the National Natural Science Foundation of China (Nos. 61875131, 61525503) and Shenzhen Basic Research Project (Nos. JCYJ20170818100931714, JCYJ20180305125549234)Shenzhen Basic Research Project JCYJ20170818100931714

Figures(9)

Single molecule localization microscopy as an advanced optical imaging technique is capable of super-resolution imaging of biological targets with the size below the optical diffraction limit. It is promising to provide powerful tools for the exploration of occurrence mechanism of severe diseases and precisely therapeutic method at single cell/organelle level, which exhibits wide applications in biomedical field. Generally, stochastic optical reconstruction microscopy (STORM) is prominently dependent on large amount of imaging buffers (Redox enzymes) and thiol-containing reagents for the ideal photoblinking behaviors of optical probes. However, the imaging buffer and thiol-containing reagents are harmful for the live cells, which make it difficult to carry out STORM imaging in live cells. Therefore, it is of significance to exploit new approaches to display STORM imaging in live cells. In this work, we provided a new strategy to facilitate the design of live cell STORM imaging probes with improved photo-blinking mechanism. A new fluorescent pentamethine cyanine probe with a thiol-attachment (SHCH₂CH₂CH₂-) at the N-position of one indoline moiety was synthesized to show spontaneously photoblinking behavior caused by intramolecular ring-closing/-opening processes. The fluorescent probe is biologically compatible with rare cytotoxicity and suitable for the live cell imaging. The probe can exhibit excellent photo-blinking under the direct illumination of a single laser beam (656 nm) with low power density (200 W·cm^-2 for solution sample and 100 W·cm^-2 for cell sample, respectively), without using any imaging buffer or thiol-chemicals. And the fluorescent probe was used to test cell toxicity with CCK-8, showed almost no cytotoxicity after 24 h incubation. The photo-blinking frames were collected with an electron multiplying charge coupled device (EMCCD, 60 Hz), and different frames were used to pre-treat with ImageJ software and then reconstruct STORM images with a Falcon algorithm to show marked imaging resolution enhancement, compared with wide-field images, which provide a new protocol for biomedical imaging.
- single-molecule localization microscopy,
- super-resolution imaging,
- pentamethine cyanine,
- photo-blinking,
- mitochondrial probe
1. [1]
  Yang, Z.; Cao, J.; He, Y.; Yang, J. H.; Kim, T.; Peng, X.; Kim, J. S. Chem. Soc. Rev. 2014, 43, 4563. doi: 10.1039/C4CS00051J
2. [2]
  Yang, Z.; Lee, J. H.; Jeon, H. M.; Han, J. H.; Park, N.; He, Y.; Lee, H.; Hong, K. S.; Kang, C.; Kim, J. S. J. Am. Chem. Soc. 2013, 135, 11657. doi: 10.1021/ja405372k
3. [3]
  Yang, Z.; He, Y.; Lee, J.-H.; Park, N.; Suh, M.; Chae, W.-S.; Cao, J.; Peng, X.; Jung, H.; Kang, C.; Kim, J. S. J. Am. Chem. Soc. 2013, 135, 9181. doi: 10.1021/ja403851p
4. [4]
  Lee, M. H.; Yang, Z.; Lim, C. W.; Lee, Y. H.; Dongbang, S.; Kang, C.; Kim, J. S. Chem. Rev. 2013, 113, 5071. doi: 10.1021/cr300358b
5. [5]
  Peng, X.; Yang, Z.; Wang, J.; Fan, J.; He, Y.; Song, F.; Wang, B.; Sun, S.; Qu, J.; Qi, J.; Yan, M. J. Am. Chem. Soc. 2011, 133, 6626. doi: 10.1021/ja1104014
6. [6]
  Huang, C.; Chen, H.; Li, F. Chin. J. Org. Chem. 2019, 39, 2467(in Chinese).
7. [7]
  Ju, Z.; Shu, P.; Xie, Z. Chin. J. Org. Chem. 2019, 39, 697(in Chinese).
8. [8]
  Xie, Z.; Fu, M.; Yin, B. Chin. J. Org. Chem. 2018, 38, 1364(in Chinese).
9. [9]
  Xi, Z.; Yuan, F.; Wang, Z. Acta Chim. Sinica 2018, 76, 460(in Chinese).
10. [10]
  Xu, Y.; Zhao, Y.; Zhang, Y. Acta Chim. Sinica 2018, 76, 393(in Chinese).
11. [11]
  Gu, T.; Shi, J.; Hua, Y. Acta Chim. Sinica 2017, 75, 991(in Chinese).
12. [12]
  Wang, Y.; Wang, W. Acta Chim. Sinica 2017, 75, 1061(in Chinese).
13. [13]
  Yang, L.; Liu, B.; Li, N. Acta Chim. Sinica 2017, 75, 1047(in Chinese).
14. [14]
  Abbe, E. Arch. Mikroskop Anat. 1873, 9, 413. doi: 10.1007/BF02956173
15. [15]
  Yang, Z.; Sharma, A.; Qi, J.; Peng, X.; Lee, D. Y.; Hu, R.; Lin, D.; Qu, J.; Kim, J. S. Chem. Soc. Rev. 2016, 45, 4651. doi: 10.1039/C5CS00875A
16. [16]
  Huang, B.; Wang, W.; Bates, M.; Zhuang, X. Science 2008, 319, 807. doi: 10.1126/science.1149860
17. [17]
  Samanta, S.; He, Y.; Sharma, A.; Kim, J.; Pan, W.; Yang, Z.; Li, J.; Yan, W.; Liu, L.; Qu, J.; Kim, J. S. CHEM 2019, 5, 1697. doi: 10.1016/j.chempr.2019.03.011
18. [18]
  Dempsey, G. T.; Bates, M.; Kowtoniuk, W. E.; Liu, D. R.; Tsien, R. Y.; Zhuang, X. J. Am. Chem. Soc. 2009, 131, 18192. doi: 10.1021/ja904588g
19. [19]
  Dempsey, G. T.; Vaughan, J. C.; Chen, K. H.; Bates, M.; Zhuang, X. Nat. Methods 2011, 8, 1027. doi: 10.1038/nmeth.1768
20. [20]
  Uno, S.; Kamiya, M.; Yoshihara, T.; Sugawara, K.; Okabe, K.; Tarhan, M. C.; Fujita, H.; Funatsu, T.; Okada, Y.; Tobita S.; Urano, Y. Nat. Chem. 2014, 6, 681. doi: 10.1038/nchem.2002
21. [21]
  Samanta, S.; Gong, W.; Li, W.; Sharma, A.; Shim, I.; Zhang, W.; Das, P.; Pan, W.; Liu, L.; Yang, Z.; Qu, J.; Kim, J. S. Coord. Chem. Rev. 2019, 380, 17. doi: 10.1016/j.ccr.2018.08.006
22. [22]
  Hell, S. W. Science 2007, 316, 1153. doi: 10.1126/science.1137395
23. [23]
  Huang, B.; Bates, M.; Zhuang, X. Ann. Rev. Biochem. 2009, 78, 993. doi: 10.1146/annurev.biochem.77.061906.092014
24. [24]
  Betzig, E.; Patterson, G. H.; Sougrat, R.; Lindwasser, O. W.; Olenych, S.; Bonifacino, J. S.; Davidson, M. W.; Lippincott-Schwartz, J.; Hess, H. F. Science 2006, 313, 5793.
25. [25]
  Rust, M. J.; Bates, M.; Zhuang, X. Nat. Methods 2006, 3, 793. doi: 10.1038/nmeth929
26. [26]
  Dertinger, T.; Colyer, R.; Iyer, G.; Weiss, S.; Enderlein, J. Proc. Natl. Acad. Sci. 2009, 106, 22287. doi: 10.1073/pnas.0907866106
27. [27]
  Bates, M.; Huang, B.; Dempsey, G. T.; Zhuang, X. Science 2007, 317, 1749. doi: 10.1126/science.1146598
28. [28]
  Mortensen, K. I.; Churchman, L. S.; Spudich, J. A.; Flyvbjerg, H. Nat. Methods 2010, 7, 3778.
29. [29]
  Huang, B.; Babcock, H.; Zhuang, X. Cell 2010, 143, 1047. doi: 10.1016/j.cell.2010.12.002
30. [30]
  Miki, K.; Kojima, K.; Oride, K.; Harada, H.; Morinibu, A.; Ohe, K. Chem. Commun. 2017, 53, 7792. doi: 10.1039/C7CC03035E
31. [31]
  Min, J.; Vonesch, C.; Kirshner, H.; Carlini, L.; Olivier, N.; Holden, S.; Manley, S.; Ye, J. C.; Unser, M. Sci. Rep. 2014, 4, 4577.
1. [1]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
2. [2]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
3. [3]
  Donghui PAN , Yuping XU , Xinyu WANG , Lizhen WANG , Junjie YAN , Dongjian SHI , Min YANG , Mingqing CHEN . Preparation and in vivo tracing of ⁶⁸Ga-labeled PM_2.5 mimetic particles for positron emission tomography imaging. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 669-676. doi: 10.11862/CJIC.20230468
4. [4]
  Yujia LI , Tianyu WANG , Fuxue WANG , Chongchen WANG . Direct Z-scheme MIL-100(Fe)/BiOBr heterojunctions: Construction and photo-Fenton degradation for sulfamethoxazole. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 481-495. doi: 10.11862/CJIC.20230314
5. [5]
  Jiakun BAI , Ting XU , Lu ZHANG , Jiang PENG , Yuqiang LI , Junhui JIA . A red-emitting fluorescent probe with a large Stokes shift for selective detection of hypochlorous acid. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1095-1104. doi: 10.11862/CJIC.20240002
6. [6]
  Zizheng LU , Wanyi SU , Qin SHI , Honghui PAN , Chuanqi ZHAO , Chengfeng HUANG , Jinguo PENG . Surface state behavior of W doped BiVO₄ photoanode for ciprofloxacin degradation. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 591-600. doi: 10.11862/CJIC.20230225
7. [7]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
8. [8]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
9. [9]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
10. [10]
  Yufang GAO , Nan HOU , Yaning LIANG , Ning LI , Yanting ZHANG , Zelong LI , Xiaofeng LI . Nano-thin layer MCM-22 zeolite: Synthesis and catalytic properties of trimethylbenzene isomerization reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1079-1087. doi: 10.11862/CJIC.20240036
11. [11]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398

Get Citation

PDF

XML

Metrics

PDF Downloads(17)
Abstract views(797)
HTML views(140)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142