Citation: Xiong Yingying, Chen Yunlong, Ju Huangxian. Glycan Analysis in Cellular Secretion[J]. Acta Chimica Sinica, ;2019, 77(12): 1221-1229. doi: 10.6023/A19080299 shu

Glycan Analysis in Cellular Secretion

State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023

Corresponding author: Ju Huangxian, hxju@nju.edu.cn
Received Date: 10 August 2019
Available Online: 9 December 2019
Fund Project: the National Natural Science Foundation of China 21635005Project supported by the National Natural Science Foundation of China (Nos. 21635005, 21827812, 21890741)the National Natural Science Foundation of China 21890741the National Natural Science Foundation of China 21827812

Figures(9)

Glycans are important components of mammalian cells, which exists extensively in eukaryocytes. Glycans are mainly consisted of monosaccharides, oligosaccharides and polysaccharides. They are connected to proteins or lipids through glycosylation, which constitute glycoconjugates. Glycosylation is one of the most important post-modifications of proteins, which mediate a wide variety of biological processes, including cell growth and differentiation, cell-cell communication, immune response, pathogen interaction, and intracellular signaling events. Because of the complex marshalling sequences, diversiform connection types and multiple branch structures, glycans are endowed with various structures. The diversity of glycan structure brings glycoconjugates with abundant information of cellular function. Among all the factors, human diseases act as an important ingredient which can induce unnatural glycosylation process. Glycoconjugates have been chosen as an efficient biomarker in the area of disease surveillance and targeted drug therapy. Thus, analysis of secreted glycans is of great importance for monitoring the states of cells or diseases in clinical diagnosis and treatment. Based on recent research of extracellular glycans, this review introduces the types of glycans in cellular secretion and their biological functions or significances, summarizes the identification or detection techniques of the secreted glycans, including lectin identifications, chemical covalent identifications and glycan metabolic marker techniques. Detection technologies of cell secretory glycan have been emphatically introduced in this review, which mainly contain spectrophotometry techniques, chromatography techniques, mass-spectrography techniques, fluorescence methods, electrochemical processes, enzyme linked immunosorbent assay techniques and western blot methods. After summarizing the progresses in this field during the past few decades, we outlook the future development of the analysis of cell secretory glycans. As far as we concern, in situ identification and quantitative detection will be the most challenging but meaningful topic of this field. We hope this review can be provided as a useful guidance for the investigating of glycosylation or glycan-related biological processes.
- glycoconjugate,
- secreted glycan,
- glycan recognition,
- glycan detection
1. [1]
  Xiao, H. P.; Suttapitugsakul, S.; Sun, F. X.; Wu, R. H. Acc. Chem. Res. 2018, 51, 1796. doi: 10.1021/acs.accounts.8b00200
2. [2]
  Zhang, L.-X.; Du, X.-F.; Zeng, Y. Acta Chim. Sinica 2016, 74, 149 (in Chinese). doi: 10.7503/cjcu20150696
3. [3]
  Krishnamoorthy, L.; Mahal, L. K. ACS Chem. Biol. 2009, 4, 715. doi: 10.1021/cb900103n
4. [4]
  Pinho, S. S.; Reis, C. A. Nat. Rev. Cancer 2015, 15, 540. doi: 10.1038/nrc3982
5. [5]
  Brockhausen, I. BBA-Gen. Subjects 1999, 1473, 67. doi: 10.1016/S0304-4165(99)00170-1
6. [6]
  Clerc, F.; Reiding, K. R.; Jansen, B. C.; Kammeijer, G. S. M.; Bondt, A.; Wuhrer, M. Glycoconjugate J. 2016, 33, 309. doi: 10.1007/s10719-015-9626-2
7. [7]
  Zhang, Z. J.; Wuhrer, M.; Holst, S. Glycoconjugate J. 2018, 35, 139. doi: 10.1007/s10719-018-9820-0
8. [8]
  Fuster, M. M.; Esko, J. D. Nat. Rev. Cancer 2005, 5, 526. doi: 10.1038/nrc1649
9. [9]
  Rich, J. R.; Withers, S. G. Nat. Chem. Biol. 2009, 5, 206. doi: 10.1038/nchembio.148
10. [10]
  Dennis, J. W.; Nabi, I. R.; Demetriou, M. Cell 2009, 139, 1229. doi: 10.1016/j.cell.2009.12.008
11. [11]
  Pinho, S. S.; Figueiredo, J.; Cabral, J.; Carvalho, S.; Dourado, J.; Magalhaes, A.; Gartner, F.; Mendonca, A. M.; Isaji, T.; Cu, J. G.; Carneiro, F.; Seruca, R.; Taniguchi, N.; Reis, C. A. BBA-Gen. Subjects 2013, 1830, 2690. doi: 10.1016/j.bbagen.2012.10.021
12. [12]
  Zhao, Y. Y.; Sato, Y.; Isaji, T.; Fukuda, T.; Matsumoto, A.; Miyoshi, E.; Gu, J. G.; Taniguchi, N. FEBS J. 2008, 275, 1939. doi: 10.1111/j.1742-4658.2008.06346.x
13. [13]
  Takeuchi, H.; Haltiwanger, R. S. Biochem. Biophys. Res. Commun. 2014, 453, 235. doi: 10.1016/j.bbrc.2014.05.115
14. [14]
  Boscher, C.; Dennis, J. W.; Nabi, I. R. Curr. Opin. Cell Biol. 2011, 23, 383. doi: 10.1016/j.ceb.2011.05.001
15. [15]
  Ju, T. Z.; Otto, V. I.; Cummings, R. D. Angew. Chem., Int. Ed. 2011, 50, 1770. doi: 10.1002/anie.201002313
16. [16]
  Gilgunn, S.; Conroy, P. J.; Saldova, R.; Rudd, P. M.; O'Kennedy, R. J. Nat. Rev. Urol. 2013, 10, 99. doi: 10.1038/nrurol.2012.258
17. [17]
  Ohtsubo, K.; Marth, J. D. Cell 2006, 126, 855. doi: 10.1016/j.cell.2006.08.019
18. [18]
  Kailemia, M. J.; Park, D.; Lebrilla, C. B. Anal. Bioanal. Chem. 2017, 409, 395. doi: 10.1007/s00216-016-9880-6
19. [19]
  Adamczyk, B.; Tharmalingam, T.; Rudd, P. M. BBA-Gen. Subjects 2012, 1820, 1347. doi: 10.1016/j.bbagen.2011.12.001
20. [20]
  Theocharis, A. D.; Skandalis, S. S.; Gialeli, C.; Karamanos, N. K. Adv. Drug Del. Rev. 2016, 97, 4. doi: 10.1016/j.addr.2015.11.001
21. [21]
  Muiznieks, L.; Keeley, F. W. Biochem. Cell Biol. 2010, 88, 392.
22. [22]
  George, E. L.; Georgeslabouesse, E. N.; Patelking, R. S.; Rayburn, H.; Hynes, R. O. Development 1993, 119, 1079.
23. [23]
  Aumailley, M.; Bruckner-Tuderman, L.; Carter, W. G.; Deutzmann, R.; Edgar, D.; Ekblom, P.; Engel, J.; Engvall, E.; Hohenester, E.; Jones, J. C. R.; Kleinman, H. K.; Marinkovich, M. P.; Martin, G. R.; Mayer, U.; Meneguzzi, G.; Miner, J. H.; Miyazaki, K.; Patarroyo, M.; Paulsson, M.; Quaranta, V.; Sanes, J. R.; Sasaki, T.; Sekiguchi, K.; Sorokin, L. M.; Talts, J. F.; Tryggvason, K.; Uitto, J.; Virtanen, I.; von der Mark, K.; Wewer, U. M.; Yamada, Y.; Yurchenco, P. D. Matrix Biol. 2005, 24, 326. doi: 10.1016/j.matbio.2005.05.006
24. [24]
  Feng, Y. M.; Guo, Y. N.; Li, Y. R.; Tao, J.; Ding, L.; Wu, J.; Ju, H. X. Anal. Chim. Acta 2018, 1039, 108. doi: 10.1016/j.aca.2018.07.040
25. [25]
  Kim, S. H.; Turnbull, J.; Guimond, S. J. Endocrinol. 2011, 209, 139. doi: 10.1530/JOE-10-0377
26. [26]
  Bonnans, C.; Chou, J.; Werb, Z. Nat. Rev. Mol. Cell Biol. 2014, 15, 786.
27. [27]
  Varki, A. Trends Mol. Med. 2008, 14, 351. doi: 10.1016/j.molmed.2008.06.002
28. [28]
  Wu, J.; Xie, X. L.; Nie, S.; Buckanovich, R. J.; Lubman, D. M. J. Proteome Res. 2013, 12, 3342. doi: 10.1021/pr400169n
29. [29]
  Saldova, R.; Royle, L.; Radcliffe, C. M.; Hamid, U. M. A.; Evans, R.; Arnold, J. N.; Banks, R. E.; Hutson, R.; Harvey, D. J.; Antrobus, R.; Petrescu, S. M.; Dwek, R. A.; Rudd, P. M. Glycobiology 2007, 17, 1344. doi: 10.1093/glycob/cwm100
30. [30]
  Ye, B.; Skates, S.; Mok, S. C.; Horick, N. K.; Rosenberg, H. F.; Vitonis, A.; Edwards, D.; Sluss, P.; Han, W. K.; Berkowitz, R. S.; Cramer, D. W. Clin. Cancer. Res. 2006, 12, 432. doi: 10.1158/1078-0432.CCR-05-0461
31. [31]
  Zhang, H.; Li, X. J.; Martin, D. B.; Aebersold, R. Nat. Biotechnol. 2003, 21, 660. doi: 10.1038/nbt827
32. [32]
  Vajaria, B. N.; Patel, K. R.; Begum, R.; Shah, F. D.; Patel, J. B.; Shukla, S. N.; Patel, P. S. Oral Surg. Oral Med. Oral Pathol. Oral Radiol. 2013, 115, 764. doi: 10.1016/j.oooo.2013.01.004
33. [33]
  Theocharis, A. D.; Gialeli, C.; Bouris, P.; Giannopoulou, E.; Skandalis, S. S.; Aletras, A. J.; Iozzo, R. V.; Karamanos, N. K. FEBS J. 2014, 281, 5023. doi: 10.1111/febs.12927
34. [34]
  Iozzo, R. V.; Sanderson, R. D. J. Cell. Mol. Med. 2011, 15, 1013. doi: 10.1111/j.1582-4934.2010.01236.x
35. [35]
  Chandler, E. M.; Seo, B. R.; Califano, J. P.; Eguiluz, R. C. A.; Lee, J. S.; Yoon, C. J.; Tims, D. T.; Wang, J. X.; Cheng, L.; Mohanan, S.; Buckley, M. R.; Cohen, I.; Nikitin, A. Y.; Williams, R. M.; Gourdon, D.; Reinhart-King, C. A.; Fischbach, C. Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 9786. doi: 10.1073/pnas.1121160109
36. [36]
  Durbeej, M. Cell Tissue Res. 2010, 339, 259. doi: 10.1007/s00441-009-0838-2
37. [37]
  Hallmann, R.; Horn, N.; Selg, M.; Wendler, O.; Pausch, F.; Sorokin, L. M. Physiol. Rev. 2005, 85, 979. doi: 10.1152/physrev.00014.2004
38. [38]
  Arcinas, A.; Yen, T. Y.; Kebebew, E.; Macher, B. A. J. Proteome Res. 2009, 8, 3958. doi: 10.1021/pr900278c
39. [39]
  Schultz, M. J.; Swindall, A. F.; Bellis, S. L. Cancer Metastasis Rev. 2012, 31, 501. doi: 10.1007/s10555-012-9359-7
40. [40]
  Bertozzi, C. R.; Kiessling, L. L. Science 2001, 291, 2357. doi: 10.1126/science.1059820
41. [41]
  Hu, Y. M.; Borges, C. R. Analyst 2017, 142, 2748. doi: 10.1039/C7AN00396J
42. [42]
  Reis, C. A.; Osorio, H.; Silva, L.; Gomes, C.; David, L. J. Clin. Pathol. 2010, 63, 322. doi: 10.1136/jcp.2009.071035
43. [43]
  Zhang, Z. J.; Wuhrer, M.; Holst, S. Glycoconjugate J. 2018, 35, 139. doi: 10.1007/s10719-018-9820-0
44. [44]
  Liu, Y. S.; He, J. T.; Li, C.; Benitez, R.; Fu, S.; Marrero, J.; Lubman, D. M. J. Proteome Res. 2010, 9, 798. doi: 10.1021/pr900715p
45. [45]
  Zeng, Y.; Ramya, T. N. C.; Dirksen, A.; Dawson, P. E.; Paulson, J. C. Nat. Methods 2009, 6, 207. doi: 10.1038/nmeth.1305
46. [46]
  Han, E.; Ding, L.; Qian, R. C.; Bao, L.; Ju, H. X. Anal. Chem. 2012, 84, 1452. doi: 10.1021/ac203489e
47. [47]
  Li, D. J.; Chen, Y.; Liu, Z. Chem. Soc. Rev. 2015, 44, 8097. doi: 10.1039/C5CS00013K
48. [48]
  Liu, L.-T.; Zhang, Y.; Jiao, J.; Yang, P.-Y.; Lu, H.-J. Acta Chim. Sinica 2013, 71, 535 (in Chinese).
49. [49]
  Liu, Z.; He, H. Acc. Chem. Res. 2017, 50, 2185. doi: 10.1021/acs.accounts.7b00179
50. [50]
  Qiu, J.; Zhang, Y.; Lu, H.-J.; Yang, P.-Y. Acta Chim. Sinica 2011, 69, 2123 (in Chinese). doi: 10.3866/PKU.WHXB20110902
51. [51]
  Ma, Y. Y.; Li, X. L.; Li, W.; Liu, Z. ACS Appl. Mater. Interfaces 2018, 10, 40918. doi: 10.1021/acsami.8b14441
52. [52]
  Wang, J.; Chen, P. Acta Chim. Sinica 2017, 75, 1173 (in Chinese).
53. [53]
  Palaniappan, K. K.; Bertozzi, C. R. Chem. Rev. 2016, 116, 14277. doi: 10.1021/acs.chemrev.6b00023
54. [54]
  Rostovtsev, V. V.; Green, L. G.; Fokin, V. V.; Sharpless, K. B. Angew. Chem., Int. Ed. 2002, 41, 2596. doi: 10.1002/1521-3773(20020715)41:14<2596::AID-ANIE2596>3.0.CO;2-4
55. [55]
  Jewett, J. C.; Bertozzi, C. R. Chem. Soc. Rev. 2010, 39, 1272. doi: 10.1039/b901970g
56. [56]
  Agard, N. J.; Baskin, J. M.; Prescher, J. A.; Lo, A.; Bertozzi, C. R. ACS Chem. Biol. 2006, 1, 644. doi: 10.1021/cb6003228
57. [57]
  Ning, X. H.; Guo, J.; Wolfert, M. A.; Boons, G. J. Angew. Chem., Int. Ed. 2008, 47, 2253. doi: 10.1002/anie.200705456
58. [58]
  Lee, T. S.; Kim, Y.; Zhang, W. Q.; Song, I. H.; Tung, C. H. Biochim. Biophys. Acta-Gen. Subj. 2018, 1862, 1091. doi: 10.1016/j.bbagen.2018.02.001
59. [59]
  Shah, M. H.; Telang, S. D.; Shah, P. M.; Patel, P. S. Glycoconjugate J. 2008, 25, 279. doi: 10.1007/s10719-007-9086-4
60. [60]
  Sawhney, H.; Kumar, C. A. Cancer Biomark. 2011, 10, 43.
61. [61]
  Rajpura, K. B.; Patel, P. S.; Chawda, J. G.; Shah, R. M. J. Oral Pathol. Med. 2005, 34, 263. doi: 10.1111/j.1600-0714.2004.00210.x
62. [62]
  Krishnan, K.; Balasundaram, S. J. Clin. Diagn. Res. 2017, 11, ZC25.
63. [63]
  Wongkham, S.; Boonla, C.; Kongkham, S.; Wongkham, C.; Bhudhisawasdi, V.; Sripa, B. Clin. Biochem. 2001, 34, 537. doi: 10.1016/S0009-9120(01)00265-X
64. [64]
  Patel, P. S.; Rawal, G. N.; Balar, D. B. Gynecol. Oncol. 1993, 50, 294. doi: 10.1006/gyno.1993.1214
65. [65]
  Vuckovic, F.; Theodoratou, E.; Thaci, K.; Timofeeva, M.; Vojta, A.; Stambuk, J.; Pucic-Bakovic, M.; Rudd, P. M.; Derek, L.; Servis, D.; Wennerstrom, A.; Farrington, S. M.; Perola, M.; Aulchenko, Y.; Dunlop, M. G.; Campbell, H.; Lauc, G. Clin. Cancer. Res. 2016, 22, 3078. doi: 10.1158/1078-0432.CCR-15-1867
66. [66]
  Saldova, R.; Royle, L.; Radcliffe, C. M.; Hamid, U. M. A.; Evans, R.; Arnold, J. N.; Banks, R. E.; Hutson, R.; Harvey, D. J.; Antrobus, R.; Petrescu, S. M.; Dwek, R. A.; Rudd, P. M. Glycobiology 2007, 17, 1344. doi: 10.1093/glycob/cwm100
67. [67]
  Kontro, H.; Joenvaara, S.; Haglund, C.; Renkonen, R. Proteomics 2014, 14, 1713. doi: 10.1002/pmic.201300270
68. [68]
  Rohrer, J. S. Anal. Biochem. 2000, 283, 3. doi: 10.1006/abio.2000.4643
69. [69]
  Raju, T. S. Curr. Opin. Immunol. 2008, 20, 471. doi: 10.1016/j.coi.2008.06.007
70. [70]
  Irani, V.; Guy, A. J.; Andrew, D.; Beeson, J. G.; Ramsland, P. A.; Richards, J. S. Mol. Immunol. 2015, 67, 171. doi: 10.1016/j.molimm.2015.03.255
71. [71]
  Selman, M. H. J.; Niks, E. H.; Titulaer, M. J.; Verschuuren, J.; Wuhrer, M.; Deelder, A. M. J. Proteome Res. 2011, 10, 143. doi: 10.1021/pr1004373
72. [72]
  Fokkink, W. J. R.; Selman, M. H. J.; Dortland, J. R.; Durmus, B.; Kuitwaard, K.; Huizinga, R.; van Rijs, W.; Tio-Gillen, A. P.; van Doorn, P. A.; Deelder, A. M.; Wuhrer, M.; Jacobs, B. C. J. Proteome Res. 2014, 13, 1722. doi: 10.1021/pr401213z
73. [73]
  Zhang, D.; Chen, B. C.; Wang, Y. M.; Xia, P.; He, C. Y.; Liu, Y. J.; Zhang, R. Q.; Zhang, M.; Li, Z. L. Sci. Rep. 2016, 6, 10. doi: 10.1038/s41598-016-0003-6
74. [74]
  Tajiri, M.; Ohyama, C.; Wada, Y. Glycobiology 2008, 18, 2. doi: 10.1093/glycob/cwm117
75. [75]
  Shi, Y.; Xu, X.; Fang, M.; Zhang, M.; Li, Y.; Gillespie, B.; Yorke, S.; Yang, N.; McKew, J. C.; Gahl, W. A.; Huizing, M.; Carrillo- Carrasco, N.; Wang, A. Q. J. Chromatogr. B 2015, 1000, 105. doi: 10.1016/j.jchromb.2015.07.018
76. [76]
  Priego-Capote, F.; Orozco-Solano, M. I.; Calderon-Santiago, M.; de Castro, M. D. L. J. Chromatogr. A 2014, 1346, 88. doi: 10.1016/j.chroma.2014.04.051
77. [77]
  Kodar, K.; Stadlmann, J.; Klaamas, K.; Sergeyev, B.; Kurtenkov, O. Glycoconjugate J. 2012, 29, 57. doi: 10.1007/s10719-011-9364-z
78. [78]
  Balmana, M.; Sarrats, A.; Llop, E.; Barrabes, S.; Saldova, R.; Ferri, M. J.; Figueras, J.; Fort, E.; de Llorens, R.; Rudd, P. M.; Peracaula, R. Clin. Chim. Acta 2015, 442, 56. doi: 10.1016/j.cca.2015.01.007
79. [79]
  Hu, Z.-Y.; Sun, Z.; Zhang, Y.; Wu, R.-A.; Zou, H.-F. Acta Chim. Sinica 2012, 70, 2059 (in Chinese).
80. [80]
  Xiong, Y.; Chen, Y.; Ding, L.; Liu, X.; Ju, H. Analyst 2019, 144, 4545. doi: 10.1039/C9AN00572B
81. [81]
  Pihikova, D.; Kasak, P.; Kubanikova, P.; Sokol, R.; Tkac, J. Anal. Chim. Acta 2016, 934, 72. doi: 10.1016/j.aca.2016.06.043
82. [82]
  Wu, J.; Xie, X. L.; Liu, Y. S.; He, J. T.; Benitez, R.; Buckanovich, R. J.; Lubman, D. M. J. Proteome Res. 2012, 11, 4541. doi: 10.1021/pr300330z
83. [83]
  Yang, W. H.; Aziz, P. V.; Heithoff, D. M.; Mahan, M. J.; Smith, J. W.; Marth, J. D. Proc. Natl. Acad. Sci. U. S. A. 2015, 112, 13657. doi: 10.1073/pnas.1515464112
1. [1]
  Dong-Bing Cheng , Junxin Duan , Haiyu Gao . Experimental Teaching Design on Chitosan Extraction and Preparation of Antibacterial Gel. University Chemistry, 2024, 39(2): 330-339. doi: 10.3866/PKU.DXHX202308053
2. [2]
  Xiaowei TANG , Shiquan XIAO , Jingwen SUN , Yu ZHU , Xiaoting CHEN , Haiyan ZHANG . A zinc complex for the detection of anthrax biomarker. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1850-1860. doi: 10.11862/CJIC.20240173
3. [3]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
4. [4]
  Junjie Zhang , Yue Wang , Qiuhan Wu , Ruquan Shen , Han Liu , Xinhua Duan . Preparation and Selective Separation of Lightweight Magnetic Molecularly Imprinted Polymers for Trace Tetracycline Detection in Milk. University Chemistry, 2024, 39(5): 251-257. doi: 10.3866/PKU.DXHX202311084
5. [5]
  Qin Hou , Jiayi Hou , Aiju Shi , Xingliang Xu , Yuanhong Zhang , Yijing Li , Juying Hou , Yanfang Wang . Preparation of Cuprous Iodide Coordination Polymer and Fluorescent Detection of Nitrite: A Comprehensive Chemical Design Experiment. University Chemistry, 2024, 39(8): 221-229. doi: 10.3866/PKU.DXHX202312056
6. [6]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
7. [7]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
8. [8]
  Di Yang , Jiayi Wei , Hong Zhai , Xin Wang , Taiming Sun , Haole Song , Haiyan Wang . Rapid Detection of SARS-CoV-2 Using an Innovative “Magic Strip”. University Chemistry, 2024, 39(4): 373-381. doi: 10.3866/PKU.DXHX202312023
9. [9]
  Hongwei Ma , Hui Li . Three Methods for Structure Determination from Powder Diffraction Data. University Chemistry, 2024, 39(3): 94-102. doi: 10.3866/PKU.DXHX202310035
10. [10]
  Feng Liang , Desheng Li , Yuting Jiang , Jiaxin Dong , Dongcheng Liu , Xingcan Shen . Method Exploration and Instrument Innovation for the Experiment of Colloid ζ Potential Measurement by Electrophoresis. University Chemistry, 2024, 39(5): 345-353. doi: 10.3866/PKU.DXHX202312009
11. [11]
  Yuting Zhang , Zhiqian Wang . Methods and Case Studies for In-Depth Learning of the Aldol Reaction Based on Its Reversible Nature. University Chemistry, 2024, 39(7): 377-380. doi: 10.3866/PKU.DXHX202311037
12. [12]
  Sifang Zhang , Yanli Tan , Yu Tao , Jiaoyan Zhao , Haihong Zhu . Exploration and Practice of Ideological and Political Cases in the Course of Chemistry History and Methodology. University Chemistry, 2024, 39(10): 377-388. doi: 10.12461/PKU.DXHX202312067
13. [13]
  Yuyang Xu , Ruying Yang , Yanzhe Zhang , Yandong Liu , Keyi Li , Zehui Wei . Research Progress of Aflatoxins Removal by Modern Optical Methods. University Chemistry, 2024, 39(11): 174-181. doi: 10.12461/PKU.DXHX202402064
14. [14]
  Jingfeng Lan , Li Wu , Guangnong Lu , Liu Yang , Xiaolong Li , Xiangyang Xu , Yongwen Shen , E Yu . Application of 3E Method in the Negative List Management System in Teaching Laboratory. University Chemistry, 2024, 39(4): 54-61. doi: 10.3866/PKU.DXHX202310130
15. [15]
  Haiping Wang . A Streamlined Method for Drawing Lewis Structures Using the Valence State of Outer Atoms. University Chemistry, 2024, 39(8): 383-388. doi: 10.12461/PKU.DXHX202401073
16. [16]
  Qilong Fang , Yiqi Li , Jiangyihui Sheng , Quan Yuan , Jie Tan . Magical Pesticide Residue Detection Test Strips: Aptamer-based Lateral Flow Test Strips for Organophosphorus Pesticide Detection. University Chemistry, 2024, 39(5): 80-89. doi: 10.3866/PKU.DXHX202310004
17. [17]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
18. [18]
  Yang YANG , Pengcheng LI , Zhan SHU , Nengrong TU , Zonghua WANG . Plasmon-enhanced upconversion luminescence and application of molecular detection. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 877-884. doi: 10.11862/CJIC.20230440
19. [19]
  Siyi ZHONG , Xiaowen LIN , Jiaxin LIU , Ruyi WANG , Tao LIANG , Zhengfeng DENG , Ao ZHONG , Cuiping HAN . Targeting imaging and detection of ovarian cancer cells based on fluorescent magnetic carbon dots. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1483-1490. doi: 10.11862/CJIC.20240093
20. [20]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120

Get Citation

PDF

XML

Metrics

PDF Downloads(9)
Abstract views(1038)
HTML views(171)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142