Citation: Zheng Qiao-Feng, Ju Zhen, Liu Shu-Shen. Combined Toxicity of Dichlorvos and Its Metabolites to Vibrio qinghaiensis sp.-Q67 and Caenorhabditis elegans[J]. Acta Chimica Sinica, ;2019, 77(10): 1008-1016. doi: 10.6023/A19060197 shu

Combined Toxicity of Dichlorvos and Its Metabolites to Vibrio qinghaiensis sp.-Q67 and Caenorhabditis elegans

College of Environmental Science and Engineering, Tongji University, Key Laboratory of Yangtze River Water Environment, Ministry of Education, Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092

Corresponding author: Liu Shu-Shen, ssliuhl@263.net
Received Date: 3 June 2019
Available Online: 13 October 2019
Fund Project: the National Natural Science Foundation of China 21677113Project supported by the National Natural Science Foundation of China (Nos. 21437004, 21677113) and the Fundamental Research Funds for the Central Universities (No. 22120180246)the National Natural Science Foundation of China 21437004the Fundamental Research Funds for the Central Universities 22120180246

Figures(5)

Pesticides and their metabolites often coexist in the real environment. The combined toxicity (synergism or antagonism) between pesticide and metabolites directly affects the environment risk assessment of pesticide. Dichlorvos (A) has three main metabolites, 2, 2-dichloroethanol (B), 2, 2-dichloroacetic acid (C) and dimethyl phosphate (D), in water and soil environment. Under different environmental conditions, metabolites with various concentration compositions form a variety of mixtures with dichlorvos. In this paper, five mixture rays with different mixture ratios were selected by optimal experimental design method. A typical aquatic (Vibrio qinghaiensis sp. -Q67) and a soil organisms (Caenorhabditis elegans) were selected as the tested organisms. The photoluminescence inhibitory toxicity (IT) of parent A and its metabolites B, C and D as well as their mixtures to Q67 and the lethal toxicity (LT) to C. elegans at different exposure time and concentration levels were determined by microplate toxicity analysis. The combination index with 95% observation-based confidence intervals was used to evaluate the change of combined toxicity of each mixture ray under different exposure times and the concentration levels. The results showed that the ITs of parent A and two metabolites C and D to Q67 do not change with the exposure time, but the IT of metabolite B at 12 h is significantly larger than that at 0.25 h. However, at two exposure times, the IT of parent A is greater than that of any of metabolites. The LTs of A and B, C and D to C. elegans do not change with the exposure time. The LTs of A, C and D to C. elegans are basically the same and significantly greater than that of B. The ITs of five mixture rays to Q67 at 12 h are significantly greater than those at 0.25 h at various concentration levels. The combined toxicities of the mixture rays to Q67 are concentration additive at low concentration levels and antagonistic at high concentration levels whether at 0.25 h or 12 h. For C. elegans, the LTs of five mixture rays at various concentration levels do not basically change with the exposure time. At two exposure times (12 h and 24 h), the combined toxicities of mixture rays are concentration additive except for the slight antagonism in the rays of R2 and R5.
- pesticide,
- multicomponent mixture,
- uniform design ray,
- basic concentration composition,
- time-dependent toxicity
1. [1]
  Cruz-Alcalde, A.; Sans, C.; Esplugas, S. Sep. Purif. Technol. 2018, 192, 123. doi: 10.1016/j.seppur.2017.09.069
2. [2]
  Park, J. A.; Abd El-Aty, A. M.; Zheng, W.; Kim, S. K.; Cho, S. H.; Choi, J. M.; Hacimuftuo, A.; Jeong, J. H.; Wang, J.; Shim, J. H.; Shin, H. C. Food Chem. 2018, 252, 40. doi: 10.1016/j.foodchem.2018.01.085
3. [3]
  Yu, Y.; Hu, S.; Yang, Y.; Zhao, X.; Xue, J.; Zhang, J.; Gao, S.; Yang, A. BMC Public Health 2017, 18, 91.
4. [4]
  Dirican, E. K.; Kalender, Y. Exp. Toxicol. Pathol. 2012, 64, 821. doi: 10.1016/j.etp.2011.03.002
5. [5]
  Oribhabor, B. J.; Ikeogu, G. C. Recent Pat. Biotechnol. 2016, 10, 272. doi: 10.2174/1872208310666160725200722
6. [6]
  Oncescu, T.; Oancea, P.; Enache, M.; Popescu, G.; Dumitru, L.; Kamekura, M. Cent. Eur. J. Biol. 2007, 2, 563. doi: 10.2478/s11535-007-0037-7
7. [7]
  Barrett, K.; Jaward, F. M. Int. J. Environ. Health Res. 2012, 22, 481. doi: 10.1080/09603123.2012.667794
8. [8]
  Boxall, A. B. A.; Sinclair, C. J.; Fenner, K.; Kolpin, D.; Maud, S. J. Environ. Sci. Technol. 2004, 38, 368A. doi: 10.1021/es040624v
9. [9]
  Benfeito, S.; Silva, T.; Garrido, J.; Andrade, P. B.; Sottomayor, M. J.; Borges, F.; Garrido, E. M. Biomed. Res. Int. 2014, no. 709036 (DOI: 10.1155/2014/709036). doi: 10.1155/2014/709036)
10. [10]
  Zhang, Q.; Ji, C.; Yan, L.; Lu, M.; Lu, C.; Zhao, M. Environ. Pollut. 2016, 218, 8. doi: 10.1016/j.envpol.2016.08.026
11. [11]
  Stancova, V.; Plhalova, L.; Bartoskova, M.; Zivna, D.; Prokes, M.; Marsalek, P.; Blahova, J.; Skoric, M.; Svobodova, Z. Biomed. Res. Int. 2014, no. 253468 (DOI: 10.1155/2014/253468). doi: 10.1155/2014/253468)
12. [12]
  Gatidou, G.; Thomaidis, N. S. Aquat. Toxicol. 2007, 85, 184. doi: 10.1016/j.aquatox.2007.09.002
13. [13]
  Liu, S. S.; Xiao, Q. F.; Zhang, J.; Yu, M. Sci. Bull. 2016, 61, 52. doi: 10.1007/s11434-015-0925-6
14. [14]
  Moser, V. C.; Simmons, J. E.; Gennings, C. Toxicol. Sci. 2006, 92, 235. doi: 10.1093/toxsci/kfj189
15. [15]
  Stork, L. G.; Gennings, C.; Carter, W. H., Jr.; Johnson, R. E.; Mays, D. P.; Simmons, J. E.; Wagner, E. D.; Plewa, M. J. J. Agric. Biol. Environ. Stat. 2007, 12, 514. doi: 10.1198/108571107X249816
16. [16]
  Fang, K. T.; Lin, D. K. J.; Winker, P.; Zhang, Y. Technometrics 2000, 42, 237. doi: 10.1080/00401706.2000.10486045
17. [17]
  Chou, T. C. Pharmacol. Rev. 2006, 58, 621. doi: 10.1124/pr.58.3.10
18. [18]
  Li, K.; Liu, S. S.; Qu, R. Asian J. Ecotoxicol. 2017, 12, 62 (in Chinese). doi: 10.7524/AJE.1673-5897.20160712001
19. [19]
  Feng, L.; Liu, S. S.; Li, K.; Tang, H. X.; Liu, H. L. J. Hazard. Mater. 2017, 327, 11. doi: 10.1016/j.jhazmat.2016.12.031
20. [20]
  Tang, H. X.; Liu, S. S.; Li, K.; Feng, L. Anal. Methods 2016, 8, 4466. doi: 10.1039/C6AY00582A
21. [21]
  Liu, L.; Liu, S. S.; Yu, M.; Chen, F. Environ. Toxicol. Pharmacol. 2015, 39, 447. doi: 10.1016/j.etap.2014.12.013
22. [22]
  Chen, Y. H.; Qin, L. T.; Mo, L. Y.; Zhao, D. N.; Zeng, H. H.; Liang, Y. P. Environ. Pollut. 2019, 250, 375. doi: 10.1016/j.envpol.2019.04.009
23. [23]
  Girotti, S.; Ferri, E. N.; Fumo, M. G.; Maiolini, E. Anal. Chim. Acta 2008, 608, 2. doi: 10.1016/j.aca.2007.12.008
24. [24]
  Wu, S.; Zhang, X.; Yang, P.; Li, L.; Tang, S. Int. J. Food Sci. Technol. 2018, 53, 2141. doi: 10.1111/ijfs.13801
25. [25]
  Jian, Q.; Gong, L.; Li, T.; Wang, Y.; Wu, Y.; Chen, F.; Qu, H.; Duan, X.; Jiang, Y. Toxins (Basel) 2017, 9, 335. doi: 10.3390/toxins9100335
26. [26]
  Thomulka, K. W.; McGee, D. J.; Lange, J. H. Bull. Environ. Contam. Toxicol. 1993, 51, 538. doi: 10.1007/BF00192169
27. [27]
  Gong, L.; Wu, Y.; Jian, Q.; Yin, C.; Li, T.; Gupta, V. K.; Duan, X.; Jiang, Y. Sci. Data 2018, 5, 170105.
28. [28]
  Ding, K.; Lu, L.; Wang, J.; Wang, J.; Zhou, M.; Zheng, C.; Liu, J.; Zhang, C.; Zhuang, S. Sci. Total Environ. 2017, 580, 1078. doi: 10.1016/j.scitotenv.2016.12.062
29. [29]
  Fan, Y.; Liu, S. S.; Qu, R.; Li, K.; Liu, H. L. RSC Adv. 2017, 7, 6080. doi: 10.1039/C6RA25843C
30. [30]
  Zhang, J.; Ding, T. T.; Dong, X. Q.; Bian, Z. Q. RSC Adv. 2018, 8, 26089. doi: 10.1039/C8RA04191A
31. [31]
  Mo, L. Y.; Liu, J.; Qin, L. T.; Zeng, H. H.; Liang, Y. P. Bull. Environ. Contam. Toxicol. 2017, 99, 17. doi: 10.1007/s00128-017-2099-1
32. [32]
  Xu, Y. Q.; Liu, S. S.; Wang, Z. J.; Li, K.; Qu, R. Ecotoxicol. Environ. Saf. 2018, 162, 304. doi: 10.1016/j.ecoenv.2018.07.007
33. [33]
  Zhou, D.; Yang, J.; Li, H.; Cui, C.; Yu, Y.; Liu, Y.; Lin, K. Chemosphere 2016, 154, 546. doi: 10.1016/j.chemosphere.2016.04.011
34. [34]
  Jager, T.; Gudmundsdottir, E. M.; Cedergreen, N. Environ. Sci. Technol. 2014, 48, 7026. doi: 10.1021/es501306t
35. [35]
  Cole, R. D.; Anderson, G. L.; Williams, P. L. Toxicol. Appl. Pharmacol. 2004, 194, 248. doi: 10.1016/j.taap.2003.09.013
36. [36]
  Yu, Z.; Yin, D.; Deng, H. Ecotoxicol. Environ. Saf. 2015, 111, 66. doi: 10.1016/j.ecoenv.2014.09.026
37. [37]
  Sochova, I.; Hofman, J.; Holoubek, I. Environ. Int. 2007, 33, 798. doi: 10.1016/j.envint.2007.03.001
38. [38]
  Varo, I.; Perini, A.; Torreblanca, A.; Garcia, Y.; Bergami, E.; Vannuccini, M. L.; Corsi, I. Sci. Total Environ. 2019, 675, 570. doi: 10.1016/j.scitotenv.2019.04.157
39. [39]
  Li, T.; Liu, S. S.; Qu, R.; Liu, H. L. Ecotoxicol. Environ. Saf. 2017, 144, 475. doi: 10.1016/j.ecoenv.2017.06.044
40. [40]
  Yu, Z. Y.; Mo, L. Y.; Zhang, J.; Liu, S. S. Chemosphere 2016, 163, 452. doi: 10.1016/j.chemosphere.2016.08.061
41. [41]
  Li, K.; Xu, Y. Q.; Feng, L.; Liu, S. S. Environ. Pollut. 2018, 242, 872. doi: 10.1016/j.envpol.2018.06.107
42. [42]
  Zhang, J.; Wang, J. C.; Liu, S. S.; Ge, H. L.; Liu, H. L. Asian J. Ecotoxicol. 2009, 4, 353 (in Chinese).
43. [43]
  Zhang, J.; Liu, S. S. Asian J. Ecotoxicol. 2012, 7, 408 (in Chinese).
44. [44]
  Zhang, J.; Liu, S. S.; Zhu, X. W. Chemosphere 2014, 112, 420. doi: 10.1016/j.chemosphere.2014.05.007
45. [45]
  Evgenidou, E.; Konstantinou, I.; Fytianos, K.; Albanis, T. J. Hazard. Mater. 2006, 137, 1056. doi: 10.1016/j.jhazmat.2006.03.042
46. [46]
  Xu, Y. Q.; Liu, S. S.; Fan, Y.; Li, K. Sci. Total Environ. 2018, 635, 432. doi: 10.1016/j.scitotenv.2018.04.023
47. [47]
  Zhang, J.; Liu, S. S.; Yu, Z. Y.; Zhang, J. Chemosphere 2013, 91, 462. doi: 10.1016/j.chemosphere.2012.11.070
48. [48]
  Zhu, X. W.; Liu, S. S.; Ge, H. L.; Liu, Y. Water Res. 2009, 43, 1731. doi: 10.1016/j.watres.2009.01.004
49. [49]
  Wang, M. C.; Liu, S. S.; Chen, F. Acta Chim. Sinica 2014, 72, 56 (in Chinese).
50. [50]
  Zhu, X. W.; Liu, S. S.; Ge, H. L.; Liu, Y. China Environ. Sci. 2009, 29, 113 (in Chinese). doi: 10.3321/j.issn:1000-6923.2009.02.001
51. [51]
  Zhu, X. W.; Liu, S. S.; Qin, L. T.; Chen, F.; Liu, H. L. Ecotoxicol. Environ. Saf. 2013, 89, 130. doi: 10.1016/j.ecoenv.2012.11.022
52. [52]
  Liu, S. S.; Zhang, J.; Zhang, Y. H.; Qin, L. T. Acta Chim. Sinica 2012, 70, 1511 (in Chinese). doi: 10.3969/j.issn.0251-0790.2012.07.027
53. [53]
  Zhang, Y. H.; Liu, S. S.; Liu, H. L.; Liu, Z. Z. Pest Manag. Sci. 2010, 66, 879. doi: 10.1002/ps.1957
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