Citation: Feng Tingting, Gao Shouqin, Wang Kun. Colorimetric Sensing of Prostate Specific Membrane Antigen Based on Gold Nanoparticles[J]. Acta Chimica Sinica, ;2019, 77(5): 422-426. doi: 10.6023/A19010018 shu

Colorimetric Sensing of Prostate Specific Membrane Antigen Based on Gold Nanoparticles

College of Traditional Chinese Medicine, Shanxi University of Chinese Medicine, Jinzhong 030619

Corresponding author: Feng Tingting, tingtingfeng1985@126.com
Received Date: 9 January 2019
Available Online: 26 May 2019
Fund Project: the Doctoral Research Start-up Fund of Shanxi University of Chinese Medicine 2014BL19Project supported by the Doctoral Research Start-up Fund of Shanxi University of Chinese Medicine (No. 2014BL19)

Figures(6)

Cancer is a major cause of death and its early diagnosis has been a research goal for many decades. For males, prostatic carcinoma has become the second leading cause of cancer death worldwide. Prostate specific membrane antigen (PSMA) has been widely recognized as a prostate cancer marker. Thus, measurement of PSMA would be more valuable for the early diagnosis of prostate cancer. Nanomaterials have the characteristics of small size effect, quantum size effect, macroscopic quantum tunneling effect and surface effect, and have been widely used in various fields, such as cell imaging, analysis and detection, drug release and treatment. Gold nanoparticles have been widely used in biosensing and medical diagnosis due to their simple preparation, high stability and unique photoelectric properties. In this paper, a new colorimetric approach is proposed for simple detection of PSMA based on gold nanoparticles. In the experiment, we synthesized gold nanoparticles with positive charges, and the polyanionic peptide as the substrate of PSMA. The detection of PSMA was based on the property that different aggregation states of gold nanoparticles can lead to the change of color and the specific recognition of PSMA for its substrate. The positively charged gold nanoparticles interact electrostatically with polyanionic peptide, resulting in aggregation of gold nanoparticles. In the presence of PSMA, however, the polyanionic peptide are hydrolyzed into glutamic acid fragment due to the reaction between the PSMA and the polyanionic peptide, resulting in the dispersion of gold nanoparticles. This behaviour leads to the development of a rapid and simple colorimetric method for assaying PSMA activity, with a detection limit of 0.5 nmol/L and the linear range of 2~10 nmol/L. This approach is simple compared to the existing ones since the gold nanoparticles-peptide based sensor is easy to be assembled and the detection can be achieved without the involvement of complicated procedures. Moreover, the applicability of the method has been demonstrated by detecting PSMA spiked into urine samples.
- gold nanoparticles,
- colorimetric sensing system,
- prostate-specific membrane antigen,
- specific recognition,
- peptide
1. [1]
  Juzgado, A.; Soldà, A.; Ostric, A.; Criado, A.; Valenti, G.; Rapino, S.; Conti, G.; Fracasso, G.; Paolucci, F.; Prato, M. J. Mater. Chem. B 2017, 5, 6681. doi: 10.1039/C7TB01557G
2. [2]
  Huang, W. F.; Chang, C. L.; Brault, N. D.; Gur, O.; Wang, Z.; Jalal, S. I.; Low, P. S.; Ratliff, T. L.; Pili, R.; Savran, C. A. Lab Chip. 2017, 17, 415. doi: 10.1039/C6LC01279E
3. [3]
  Ferraris, D. V.; Shukla, K.; Tsukamoto, T. Curr. Med. Chem. 2012, 19, 1282. doi: 10.2174/092986712799462658
4. [4]
  Yang, H. W.; Hua, M. Y.; Liu, H. L.; Tsai, R. Y.; Chuang, C. K.; Chu, P. C.; Wu, P. Y.; Chang, Y. H.; Chuang, H. C.; Yu, K. J.; Pang, S. T. ACS Nano 2012, 6, 1795. doi: 10.1021/nn2048526
5. [5]
  Pu, F.; Salarian, M.; Xue, S. H.; Qiao, J. J.; Feng, J.; Tan, S. S.; Patel, A.; Li, X.; Mamouni, K.; Hekmatyar, K.; Zou, J.; Wu, D. Q.; Yang, J. J. Nanoscale 2016, 8, 12668. doi: 10.1039/C5NR09071G
6. [6]
  Min, K.; Song, K. M.; Cho, M.; Chun, Y. S.; Shim, Y. B.; Ku, J. K.; Ban, C. Chem. Commun. 2010, 46, 5566. doi: 10.1039/c002524k
7. [7]
  Carter, R. E.; Feldman, A. R.; Coyle, J. T. Proc. Natl. Acad. Sci. U. S. A. 1996, 93, 749. doi: 10.1073/pnas.93.2.749
8. [8]
  Kamga, I.; Ng, R.; Hosaka, M.; Berkman, C. E. Anal. Biochem. 2002, 310, 125. doi: 10.1016/S0003-2697(02)00284-1
9. [9]
  Wang, H. S.; Lin, P. T.; Zhao, S. L.; Li, S. T.; Lu, X.; Liang, H. Chin. J. Chem. 2017, 35, 943. doi: 10.1002/cjoc.v35.6
10. [10]
  Yang, L. M.; Liu, B.; Li, N.; Tang, B. Acta Chim. Sinica 2017, 75, 1047.
11. [11]
  Li, M. B.; Tian, S. K.; Wu, Z. K. Chin. J. Chem. 2017, 35, 567. doi: 10.1002/cjoc.v35.5
12. [12]
  Li, S. S.; Wang, F. Z. R.; Liu, Y. M.; Cao, Y. Chin. J. Chem. 2017, 35, 591. doi: 10.1002/cjoc.v35.5
13. [13]
  Zhu, A.W.; Qu, Q.; Shao, X. L.; Kong, B.; Tian, Y. Angew. Chem., Int. Ed. 2012, 51, 7185. doi: 10.1002/anie.201109089
14. [14]
  Zhang, Y. Y.; Wu, M. H.; Wu, M. J.; Guo, L. P.; Cao, L., Wu, H. Y.; Zhang, X. N. Acta Chim. Sinica 2018, 76, 709.
15. [15]
  Ji, G.; Yan, L. L.; Wang, H.; Ma, L.; Xu, B.; Tian, W. J. Acta Chim. Sinica 2016, 74, 917.
16. [16]
  Men, J. Y.; Gao, B. J.; Chen, Z. P.; Yao, L. Acta Chim. Sinica 2012, 70, 2273.
17. [17]
  Wen, Q. S.; Tang, H. W.; Yang, G. M.; Liu, L. B.; Lv, F. T.; Yang, Q.; Wang, S. Acta Chim. Sinica 2012, 70, 2137.
18. [18]
  Elghanian, R.; Storhoff, J. J.; Mucic, R. C.; Letsinger, R. L.; Mirkin, C. A. Science 1997, 277, 1078. doi: 10.1126/science.277.5329.1078
19. [19]
  Taton, T. A.; Mirkin, C. A.; Letsinger, R. L. Science 2000, 289, 1757. doi: 10.1126/science.289.5485.1757
20. [20]
  Fratoddi, I.; Venditti, I.; Cametti, C.; Russo, M. V. J. Mater. Chem. B 2014, 2, 4204. doi: 10.1039/C4TB00383G
21. [21]
  Obare, S. O.; Hollowell, R. E.; Murphy, C. J. Langmuir 2002, 18, 10407. doi: 10.1021/la0260335
22. [22]
  Kim, Y.; Johnson, R. C.; Hupp, J. T. Nano Lett. 2001, 1, 165. doi: 10.1021/nl0100116
23. [23]
  Zhou, Y.; Wang, S.; Zhang, K.; Jiang, X. Angew. Chem., Int. Ed. 2008, 47, 7454. doi: 10.1002/anie.v47:39
24. [24]
  Anderson, M. O.; Wu, L. Y.; Santiago, N. M. Bioorgan. Med. Chem. 2007, 15, 6678. doi: 10.1016/j.bmc.2007.08.006
25. [25]
  Feng, D.; Zhang, Y. Y.; Feng, T. T.; Shi, W.; Li, X. H.; Ma, H. M. Chem. Commun. 2011, 47, 10680. doi: 10.1039/c1cc13975d
26. [26]
  Miao, X. M.; Cheng, Z. Y.; Li, Z. B.; Wang, P. Biochem. Eng. J. 2017, 117, 21. doi: 10.1016/j.bej.2016.10.022
27. [27]
  Sun, C. D.; Shi, W.; Song, Y. C.; Chen, W.; Ma, H. M. Chem. Commun. 2011, 47, 8638. doi: 10.1039/c1cc12174j
28. [28]
  Mohan, K. M.; Donavan, K. C.; Arter, J. A.; Penner, R. M.; Weiss, G. A. J. Am. Chem. Soc. 2013, 135, 7761. doi: 10.1021/ja4028082
29. [29]
  Li, C. M.; Li, Y. F.; Wang, J.; Huang, C. Z. Talanta 2010, 81, 1339.
1. [1]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
2. [2]
  Yan LIU , Jiaxin GUO , Song YANG , Shixian XU , Yanyan YANG , Zhongliang YU , Xiaogang HAO . Exclusionary recovery of phosphate anions with low concentration from wastewater using a CoNi-layered double hydroxide/graphene electronically controlled separation film. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1775-1783. doi: 10.11862/CJIC.20240043
3. [3]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
4. [4]
  Chenye An , Abiduweili Sikandaier , Xue Guo , Yukun Zhu , Hua Tang , Dongjiang Yang . 红磷纳米颗粒嵌入花状CeO₂分级S型异质结高效光催化产氢. Acta Physico-Chimica Sinica, 2024, 40(11): 2405019-. doi: 10.3866/PKU.WHXB202405019
5. [5]
  Baohua LÜ , Yuzhen LI . Anisotropic photoresponse of two-dimensional layered α-In₂Se₃(2H) ferroelectric materials. Chinese Journal of Inorganic Chemistry, 2024, 40(10): 1911-1918. doi: 10.11862/CJIC.20240105
6. [6]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
7. [7]
  Jingke LIU , Jia CHEN , Yingchao HAN . Nano hydroxyapatite stable suspension system: Preparation and cobalt adsorption performance. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1763-1774. doi: 10.11862/CJIC.20240060
8. [8]
  Jiao CHEN , Yi LI , Yi XIE , Dandan DIAO , Qiang XIAO . Vapor-phase transport of MFI nanosheets for the fabrication of ultrathin b-axis oriented zeolite membranes. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 507-514. doi: 10.11862/CJIC.20230403
9. [9]
  Zian Fang , Qianqian Wen , Yidi Wang , Hongxia Ouyang , Qi Wang , Qiuping Li . The Test Paper for Metal Ion: A Popular Science Experiment Based on Color Aesthetics. University Chemistry, 2024, 39(5): 108-115. doi: 10.3866/PKU.DXHX202310032
10. [10]
  Qin ZHU , Jiao MA , Zhihui QIAN , Yuxu LUO , Yujiao GUO , Mingwu XIANG , Xiaofang LIU , Ping NING , Junming GUO . Morphological evolution and electrochemical properties of cathode material LiAl_0.08Mn_1.92O₄ single crystal particles. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1549-1562. doi: 10.11862/CJIC.20240022
11. [11]
  Tengjiao Wang , Tian Cheng , Rongjun Liu , Zeyi Wang , Yuxuan Qiao , An Wang , Peng Li . Conductive Hydrogel-based Flexible Electronic System: Innovative Experimental Design in Flexible Electronics. University Chemistry, 2024, 39(4): 286-295. doi: 10.3866/PKU.DXHX202309094
12. [12]
  Fang Niu , Rong Li , Qiaolan Zhang . Analysis of Gas-Solid Adsorption Behavior in Resistive Gas Sensing Process. University Chemistry, 2024, 39(8): 142-148. doi: 10.3866/PKU.DXHX202311102
13. [13]
  Tiantian MA , Sumei LI , Chengyu ZHANG , Lu XU , Yiyan BAI , Yunlong FU , Wenjuan JI , Haiying YANG . Methyl-functionalized Cd-based metal-organic framework for highly sensitive electrochemical sensing of dopamine. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 725-735. doi: 10.11862/CJIC.20230351
14. [14]
  Xiaowu Zhang , Pai Liu , Qishen Huang , Shufeng Pang , Zhiming Gao , Yunhong Zhang . Acid-Base Dissociation Equilibrium in Multiphase System: Effect of Gas. University Chemistry, 2024, 39(4): 387-394. doi: 10.3866/PKU.DXHX202310021
15. [15]
  Peiran ZHAO , Yuqian LIU , Cheng HE , Chunying DUAN . A functionalized Eu³⁺ metal-organic framework for selective fluorescent detection of pyrene. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 713-724. doi: 10.11862/CJIC.20230355
16. [16]
  Lu XU , Chengyu ZHANG , Wenjuan JI , Haiying YANG , Yunlong FU . Zinc metal-organic framework with high-density free carboxyl oxygen functionalized pore walls for targeted electrochemical sensing of paracetamol. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 907-918. doi: 10.11862/CJIC.20230431
17. [17]
  Jing SU , Bingrong LI , Yiyan BAI , Wenjuan JI , Haiying YANG , Zhefeng Fan . Highly sensitive electrochemical dopamine sensor based on a highly stable In-based metal-organic framework with amino-enriched pores. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1337-1346. doi: 10.11862/CJIC.20230414
18. [18]
  Jiarong Feng , Yejie Duan , Chu Chu , Dezhen Xie , Qiu'e Cao , Peng Liu . Preparation and Application of a Streptomycin Molecularly Imprinted Electrochemical Sensor: A Suggested Comprehensive Analytical Chemical Experiment. University Chemistry, 2024, 39(8): 295-305. doi: 10.3866/PKU.DXHX202401016
19. [19]
  Jinlong YAN , Weina WU , Yuan WANG . A simple Schiff base probe for the fluorescent turn-on detection of hypochlorite and its biological imaging application. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1653-1660. doi: 10.11862/CJIC.20240154
20. [20]
  Zhenyu Feng , Zhaozhen Cao , Jinhua Zhan . Exploration of Online Training System for Large-Scale Instrument in Open Laboratory of Universities. University Chemistry, 2024, 39(4): 1-6. doi: 10.3866/PKU.DXHX202311016

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(648)
HTML views(149)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142