Citation: Duan Yu, Chen Xin, Shao Zhengzhong. Preparation and Properties of Antheraea pernyi/Bombyx mori Silk Fibroin Blending Scaffold[J]. Acta Chimica Sinica, ;2018, 76(3): 190-195. doi: 10.6023/A17110511 shu

Preparation and Properties of Antheraea pernyi/Bombyx mori Silk Fibroin Blending Scaffold

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai 200433

Corresponding author: Shao Zhengzhong, zzshao@fudan.edu.cn
Received Date: 28 November 2017
Available Online: 29 March 2017
Fund Project: Project supported by the National Natural Science Foundation of China (No. 21574024)the National Natural Science Foundation of China 21574024

Figures(6)

Lots of research has indicated that materials contain Arg-Gly-Asp (RGD) sequence can promote cell attachment and proliferation on them. Although Antheraea pernyi silk fibroin is a natural structural protein which contains RGD sequence, there are few studies on this kind of protein materials, for the regeneration of Antheraea pernyi silk fibroin from silk fibers is complicated and it is hard to be processed. In this paper, we present a water-insoluble Antheraea pernyi/Bombyx mori silk fibroin blending scaffold. The regenerated Antheraea pernyi silk fibroin (RASF) solution was prepared by dissolving degummed silk fibers at 100℃ and dialyzing at 4℃. The regenerated Bombyx mori silk fibroin (RBSF) solution was prepared by dissolving degummed silk fibers at 60℃ and dialyzing at 20℃. Regenerated silk fibroin solution was concentrated to 6 wt% solution in 10 wt% PEG solution. Based on RASF and RBSF solution, RBSF porous scaffold and RASF/RBSF blending scaffolds with different ratios were prepared through treating 1-butanol/SF solution under freezing at -20℃. The volume ratio of 1-butanol to solution was 1:2. RASF porous scaffold was not hard enough to hold itself, therefore the maximum content of RASF in blending scaffold was 70 wt%. With increasing of RASF content, pore sizes of scaffolds decreased from 250 μm to 150 μm and compressive strengths decreased from 280 kPa to 108 kPa, while the thermal stabilities increased. FTIR results demonstrated that the molecular conformation of silk fibroin was proven to be β-sheet, β-turn and α-helix. The biocompatibilities of scaffolds were demonstrated with in vitro cell culture. The results showed that L929 fibroblast and MC3t3-E1 osteoblast adhered, proliferated and migrated well into the scaffolds. The speed of cell proliferation accelerated with the increase of RASF content. Obviously, these regenerated silk fibroin scaffolds with good bio-compatibility could be used in tissue engineering field further.
- animal silk protein,
- sequence,
- tissue engineering,
- compatibility,
- mechanical properties
1. [1]
  For a recent review: (a) Whang, K. ; Thomas, C. ; Healy, K. ; Nuber, G. Polymer 1995, 36, 837; (b) Kim, H. J. ; Kim, U. J. ; Kim, H. S. ; Li, C. ; Wada, M. ; Leisk, G. G. ; Kaplan, D. L. Bone 2008, 42, 1226; (c) Melke, J. ; Midha, S. ; Ghosh, S. ; Ito, K. ; Hofmann, S. Acta Biomater. 2016, 31, 1.
2. [2]
  For a recent review: (a) Ma, Z. ; Kotaki, M. ; Inai, R. ; Ramakrishna, S. Tissue. Eng. 2005, 11, 101; (b) Kalaf, E. A. G. ; Flores, R. ; Bledsoe, J. G. ; Sell, S. A. Mater. Sci. Eng. C 2016, 63, 198; (c) Bhardwaj, N. ; Singh, Y. P. ; Devi, D. ; Kandimalla, R. ; Kotoky, J. ; Mandal, B. B. J. Mater. Chem. B 2016, 4, 3670.
3. [3]
  Altman, G. H.; Diaz, F.; Jakuba, C.; Calabro, T.; Horan, R. L.; Chen, J.; Lu, H.; Richmond, J.; Kaplan, D. L. Biomaterials 2003, 24, 401. doi: 10.1016/S0142-9612(02)00353-8
4. [4]
  Unger, R. E.; Ghanaati, S.; Orth, C.; Sartoris, A.; Barbeck, M.; Halstenberg, S.; Motta, A.; Migliaresi, C.; Kirkpatrick, C. J. Biomaterials 2010, 31, 6959. doi: 10.1016/j.biomaterials.2010.05.057
5. [5]
  Mandal, B. B.; Grinberg, A.; Gil, E. S.; Panilaitis, B.; Kaplan, D. L. PNAS 2012, 109, 7699. doi: 10.1073/pnas.1119474109
6. [6]
  Minoura, N.; Aiba, S. I.; Higuchi, M.; Gotoh, Y.; Tsukada, M.; Imai, Y. Biochem. Biophys. Res. Commun. 1995, 208, 511. doi: 10.1006/bbrc.1995.1368
7. [7]
  (a) Kroese-Deutman, H. ; Den Dolder, J. V. ; Spauwen, P. ; Jansen, J. Tissue. Eng. 2005, 11, 1867; (b) Oya, K. ; Tanaka, Y. ; Saito, H. ; Kurashima, K. ; Nogi, K. ; Tsutsumi, H. ; Tsutsumi, Y. ; Doi, H. ; Nomura, N. ; Hanawa, T. Biomaterials 2009, 30, 1281.
8. [8]
  Li, M.; Tao, W.; Lu, S.; Zhao, C. Polym. Adv. Technol. 2008, 19, 207. doi: 10.1002/(ISSN)1099-1581
9. [9]
  Cao, Z.-B.; Wen, J.-C.; Yao, J.-R.; Chen, X.; Ni, Y.; Shao, Z.-Z. Mater. Sci. Eng. C 2013, 33, 3522. doi: 10.1016/j.msec.2013.04.045
10. [10]
  Zhang, F.; Zuo, B.-Q.; Zhang, H.-X.; Bai, L. Polymer 2009, 50, 279. doi: 10.1016/j.polymer.2008.10.053
11. [11]
  Wang, Q.; Chen, Q.; Yang, Y.-H.; Shao, Z.-Z. Biomacromolecules 2012, 14, 285.
12. [12]
  (a) Madihally, S. V. ; Matthew, H. W. Biomaterials 1999, 20, 1133; (b) Hollister, S. J. Nat. Mater. 2005, 4, 518.
13. [13]
  (a) Freddi, G. ; Monti, P. ; Nagura, M. ; Gotoh, Y. ; Tsukada, M. J. Polym. Sci. Part B: Polym. Phys. 1997, 35, 841; (b) Chen, X. ; Shao, Z. -Z. ; Marinkovic, N. S. ; Miller, L. M. ; Zhou, P. ; Chance, M. R. Biophys. Chem. 2001, 89, 25.
14. [14]
  Chen, X.; Knight, D. P.; Shao, Z.-Z. Soft Matter 2009, 5, 2777. doi: 10.1039/b900908f
15. [15]
  Yu, Q.; Zhou, J.; Fung, Y. C. Am. J. Physiol. 1993, 265, 52.
16. [16]
1. [1]
  Feng Zheng , Ruxun Yuan , Xiaogang Wang . “Research-Oriented” Comprehensive Experimental Design in Polymer Chemistry: the Case of Polyimide Aerogels. University Chemistry, 2024, 39(10): 210-218. doi: 10.12461/PKU.DXHX202404027
2. [2]
  Jin Tong , Shuyan Yu . Crystal Engineering for Supramolecular Chirality. University Chemistry, 2024, 39(3): 86-93. doi: 10.3866/PKU.DXHX202308113
3. [3]
  Xiaohui Li , Ze Zhang , Jingyi Cui , Juanjuan Yin . Advanced Exploration and Practice of Teaching in the Experimental Course of Chemical Engineering Thermodynamics under the “High Order, Innovative, and Challenging” Framework. University Chemistry, 2024, 39(7): 368-376. doi: 10.3866/PKU.DXHX202311027
4. [4]
  Keying Qu , Jie Li , Ziqiu Lai , Kai Chen . Unveiling the Mystery of Chirality from Tartaric Acid. University Chemistry, 2024, 39(9): 369-378. doi: 10.12461/PKU.DXHX202310091
5. [5]
  Yan Li , Xinze Wang , Xue Yao , Shouyun Yu . Kinetic Resolution Enabled by Photoexcited Chiral Copper Complex-Mediated Alkene E→Z Isomerization: A Comprehensive Chemistry Experiment for Undergraduate Students. University Chemistry, 2024, 39(5): 1-10. doi: 10.3866/PKU.DXHX202309053
6. [6]
  Jin CHANG . Supercapacitor performance and first-principles calculation study of Co-doping Ni(OH)₂. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1697-1707. doi: 10.11862/CJIC.20240108
7. [7]
  Kai Yang , Gehua Bi , Yong Zhang , Delin Jin , Ziwei Xu , Qian Wang , Lingbao Xing . Comprehensive Polymer Chemistry Experiment Design: Preparation and Characterization of Rigid Polyurethane Foam Materials. University Chemistry, 2024, 39(4): 206-212. doi: 10.3866/PKU.DXHX202308045
8. [8]
  Jiarui Wu , Gengxin Wu , Yan Wang , Yingwei Yang . Crystal Engineering Based on Leaning Towerarenes. University Chemistry, 2024, 39(3): 58-62. doi: 10.3866/PKU.DXHX202304014
9. [9]
  Cheng PENG , Jianwei WEI , Yating CHEN , Nan HU , Hui ZENG . First principles investigation about interference effects of electronic and optical properties of inorganic and lead-free perovskite Cs₃Bi₂X₉ (X=Cl, Br, I). Chinese Journal of Inorganic Chemistry, 2024, 40(3): 555-560. doi: 10.11862/CJIC.20230282
10. [10]
  Tingyu Zhu , Hui Zhang , Wenwei Zhang . Exploration and Practice of Ideological and Political Education in the Course of Experiments on Chemical Functional Molecules: Synthesis and Catalytic Performance Study of Chiral Mn(III)Cl-Salen Complex. University Chemistry, 2024, 39(4): 75-80. doi: 10.3866/PKU.DXHX202311011
11. [11]
  Meng Lin , Hanrui Chen , Congcong Xu . Preparation and Study of Photo-Enhanced Electrocatalytic Oxygen Evolution Performance of ZIF-67/Copper(I) Oxide Composite: A Recommended Comprehensive Physical Chemistry Experiment. University Chemistry, 2024, 39(4): 163-168. doi: 10.3866/PKU.DXHX202308117
12. [12]
  Zeyu XU , Anlei DANG , Bihua DENG , Xiaoxin ZUO , Yu LU , Ping YANG , Wenzhu YIN . Evaluation of the efficacy of graphene oxide quantum dots as an ovalbumin delivery platform and adjuvant for immune enhancement. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1065-1078. doi: 10.11862/CJIC.20240099
13. [13]
  Xinyi Hong , Tailing Xue , Zhou Xu , Enrong Xie , Mingkai Wu , Qingqing Wang , Lina Wu . Non-Site-Specific Fluorescent Labeling of Proteins as a Chemical Biology Experiment. University Chemistry, 2024, 39(4): 351-360. doi: 10.3866/PKU.DXHX202310010
14. [14]
  Lei Shi . Nucleophilicity and Electrophilicity of Radicals. University Chemistry, 2024, 39(11): 131-135. doi: 10.3866/PKU.DXHX202402018
15. [15]
  Shuyong Zhang , Shu'e Song . Ideological and Political Case Design of Experiment of Corrosion and Protection Linking with National Major Projects. University Chemistry, 2024, 39(2): 57-60. doi: 10.3866/PKU.DXHX202304078
16. [16]
  Shahua Huang , Xiaoming Guo , Lin Lin , Guangping Chang , Sheng Han , Zuxin Zhou . Application of “Integration of Industry and Education” in Engineering Chemistry: Improvement of the Pesticide Fipronil Production. University Chemistry, 2024, 39(3): 199-204. doi: 10.3866/PKU.DXHX202309064
17. [17]
  Shule Liu . Application of SPC/E Water Model in Molecular Dynamics Teaching Experiments. University Chemistry, 2024, 39(4): 338-342. doi: 10.3866/PKU.DXHX202310029
18. [18]
  Yaling Chen . Basic Theory and Competitive Exam Analysis of Dynamic Isotope Effect. University Chemistry, 2024, 39(8): 403-410. doi: 10.3866/PKU.DXHX202311093
19. [19]
  Yongming Zhu , Huili Hu , Yuanchun Yu , Xudong Li , Peng Gao . Construction and Practice on New Form Stereoscopic Textbook of Electrochemistry for Energy Storage Science and Engineering: Taking Basic Course of Electrochemistry as an Example. University Chemistry, 2024, 39(8): 44-47. doi: 10.3866/PKU.DXHX202312086
20. [20]
  Qingying Gao , Tao Luo , Jianyuan Su , Chaofan Yu , Jiazhu Li , Bingfei Yan , Wenzuo Li , Zhen Zhang , Yi Liu . Refinement and Expansion of the Classic Cinnamic Acid Synthesis Experiment. University Chemistry, 2024, 39(5): 243-250. doi: 10.3866/PKU.DXHX202311074

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(1224)
HTML views(208)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142