Citation: Cao Chan, Liao Dongfang, Ying Yilun, Long Yitao. Detection of Single Oligonucleotide by an Aerolysin Nanopore[J]. Acta Chimica Sinica, ;2016, 74(9): 734-737. doi: 10.6023/A16070352 shu

Detection of Single Oligonucleotide by an Aerolysin Nanopore

1.
Key Laboratory for Advanced Materials, School of Chemistry & Molecular Engineering, East China University of Science and Technology, Shanghai 200237

Received Date: 19 July 2016

Fund Project: the Program of Introducing Talents of Discipline to Universities B16017the National Natural Science Foundation of China 21421004the National Natural Science Foundation of China 21327807

Figures(3)

Since the nanopore single-molecule technology has been proposed, it remains a big challenge to generate a sensitive and stable nano-scale pore. In order to achieve this goal, membrane proteins, solid-state nanopore and other materials such as DNA origami have been involved to fabricate a suitable nanopore. Compared to the solid-state nanopores, biological nanopores perform a higher resolution for single molecule analysis. Therefore, the investigation of finding new biological nanopores is very important to realize the discrimination of single oligonucleotide. Aerolysin biological nanopore has been applied for the study of oligosaccharides, peptides, protein unfolding and small organic molecules so far. Here, we report that Aerolysin could be utilized for oligonucleotide analysis. The data demonstrated that Aerolysin nanopore has a high resolution both for current and time compared with other most widely used wild-type biological nanopores, such as α-hemolysin and Mycobacterium smegmatis porin A (MspA). It may be because of its narrow diameter and positive charged amino acids in the lumen. One Aerolysin pore generates a 50 pA constant ion current in 1 mol/L KCl solution, as a three nucleotides length oligonucleotides (5'-AGG-3') traversing through nanopore could induce nearly 40% current blockage. In comparison, no current blockage signals were observed when 5'-AGG-3' driven from either cis or trans side of the α-hemolysin nanopore. Furthermore, the statistical analysis of duration time of single oligonucleotide through Aerolysin indicates a relationship scale with applied voltage, as the voltage increased from 80 to 160 mV, the duration gradually decreased. Although Aerolysin nanopore has been investigated for nearly 10 years, its ability to detect oligonucleotide was not highlighted. Our findings explored high sensing capabilities of Aerolysin nanopore in the analysis of single oligonucleotide, and extended its application to single-molecule nucleic acid analysis. Aerolysin is a promising candidate for the DNA sensing, DNA damage detection, microRNA analysis and other single molecule investigations.
- Aerolysin,
- biological nanopores,
- single-molecule detection,
- oligonucleotides
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1.
沈阳化工大学材料科学与工程学院沈阳 110142