Citation: LI Qiang, JI Xiao-Xu, ZHONG Qiu, HUANG Xin-Tang, XIONG Li. Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(11): 2375-2381. doi: 10.3969/j.issn.1001-4861.2013.00.368 shu

Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins

1.
1 Institute of Nanoscience and Nanotechnology, Central China Normal University, Wuhan 430079, China;

Received Date: 8 May 2013
Available Online: 20 June 2013
Fund Project: 国家自然科学基金(No.51172085)资助项目。 (No.51172085)

Ultrafine ZnFe₂O₄ nanocrystals were prepared by hydrothermal method and characterized by HRTEM, XRD and EDX techniques respectively. Protein adsorption properties and their correlations to ζ potentials between nanocrystals and protein molecules were investigated under different pH conditions using bovine serum albumin (BSA) and hemoglobin as model proteins. The hydrodynamic size of bare and protein loaded nanocrystals as well as protein conformation changes induced by nanocrystals were respectively studied by dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy techniques. Results show that adsorption between nanocrystals and hemoglobin obeys the law of electrostatistic interaction, whereas BSA adsorption behavior is not agreed with such law. After hemoglobin loading, most of nanocrystal-protein systems suspend as monomers and trimers apart from a few aggregates, whereas only aggregates exist after BSA adsorbing onto nanocrystals. FTIR spectroscopy revealed that Hemoglobin suffers more conformational changes than that of BSA. In addition, highly protein adsorption capacities exceeding₃₈₀ mg·g^-1 at appropriate pH conditions imply the potential applications of nanocrystals in protein separation.
- ZnFe₂O₄ nanocrystal;bovine serum albumin (BSA);hemoglobin;interactions
1. [1]
  [1] Mahmoudi M, Lynch I, Ejtehadi M R, et al. Chem. Rev., 2011,111:5610-5637
2. [2]
  [2] Nel A E, Mdler L, Velegol D, et al. Nat. Mater., 2009,8:543-557
3. [3]
  [3] Ge C C, Du J F, Zhao L, et al. PNAS, 2011,108:16968-16973
4. [4]
  [4] Hu W B, Peng C, Lü M, et al. ACS Nano, 2011,5:3693-3700
5. [5]
  [5] Sun S G, Ma M H, Qiu N, et al. Colloids. Surf. B Biointerfaces, 2011,88:246-253
6. [6]
  [6] DU Chong-Lei(杜崇磊), DU Wei(杜伟), WANG Bing(汪冰), et al. Chin. J. Anal. Chem.(Fenxi Huaxue), 2010,38(6):902-908
7. [7]
  [7] ZHAO Zi-Lai(赵紫来), BIAN Zheng-Yun(卞征云), CHEN Lang-Xing(陈朗星), et al. Prog. Chem.(Huaxue Jinzhan), 2006,18(10):1288-1297
8. [8]
  [8] HUANG Shan-Sheng(黄杉生), YIN Yue-Fen(殷月芬), WANG Ke-Min(王柯敏), et al. Chem. J. Chinese Universities (Gaodeng Xuexiao Huaxue Xuebao), 2004,25(12):2238-2241
9. [9]
  [9] HUANG Tian-Tian(黄天天), FU Yan(付雁), ZHANG Jin-Li (张金利), et al. Prog. Chem.(Huaxue Jinzhan), 2012,24(8): 1610-1622
10. [10]
  [10]ZHANG Huai(张怀), ZHANG Yun-Huai(张云怀), LI Jing (李静), et al. Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3): 253-259
11. [11]
  [11]NI Song-Bo(倪淞波), LI Yan-Bao(李延报), WANG Xiu-Mei (王秀梅). Prog. Chem.(Huaxue Jinzhan), 2011,23(1):231-245
12. [12]
  [12]LÜYa-Fen(吕亚芬), CAI Cheng-Xin(蔡称心). Acta Chim. Sinica(Huaxue Xuebao), 2006,64(24):2396-2402
13. [13]
  [13]ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33(7):1394-1400
14. [14]
  [14]LI Ya-Ru(李亚茹), ZHANG Xue-Lian(张雪莲), PAN Yuan-Yuan(潘园园), et al. J. Funct. Mater.(Gongneng Cailiao), 2012,43(8):1045-1048
15. [15]
  [15]WU Wei(吴伟), HE Quan-Guo(贺全国), CHEN Hong(陈洪). Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3):265-272
16. [16]
  [16]Xu M, Li J, Iwai H, et al. Sci. Rep., 2012,2:1-6
17. [17]
  [17]YANG Xiao-Chao(杨小超), MO Zhi-Hong(莫志宏). Acta Chim. Sinica(Huaxue Xuebao), 2010,68(15):1549-1552
18. [18]
  [18]ZHANG Li(张莉), TANG Xin-Feng(唐新峰), GAO Wen-Bin (高文斌). J. Inorg. Mater.(Wuji Cailiao Xuebao), 2008,23(4): 860-864
19. [19]
  [19]YUAN Yuan(袁媛), HE Xiao-Xiao(何晓晓), SHI Hui(石慧), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(11):2167-2172
20. [20]
  [20]Gan X, Liu T, Zhong J, et al. Chem. Bio. Chem., 2004,5: 1686-1691
21. [21]
  [21]Zhou H, Gan X, Liu T, et al. J. Biochem. Biophys. Methods, 2005,64:38-45
22. [22]
  [22]Zhu X, Yuri I, Gan X, et al. Biosens. Bioelectron., 2007,22: 1600-1604
23. [23]
  [23]Zhou H, Gan X, Liu T, et al. Bioelectrochemistry, 2006,69: 34-40
24. [24]
  [24]SHAN Hong-Yan(单洪岩), LIU Dian-Jun(刘殿骏), WANG Zhen-Xin(王振新). Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(12):2372-2374
25. [25]
  [25]MENG Jie(孟洁), YANG Man(杨曼), WANG Chao-Ying(王超英), et al. New Carbon Mater.(Xinxing Tancailiao), 2007, 22(3):218-226
26. [26]
  [26]JU Si-Ting(鞠思婷), PU Ling-Yu(朴玲钰), YANG Lei(杨磊), et al. Prog. Chem.(Huaxue Jinzhan), 2010,22(9):1767-1775
27. [27]
  [27]GUO Xin-Lu(过馨露), ZHANG Jian(张建), WANG Wei(王炜). Prog. Phys.(Wulixue Jinzhan), 2012,32(6):285-293
28. [28]
  [28]TANG Shi-Hua(唐世华), HUANG Jian-Bin(黄建滨). Acta Chim. Sinica(Huaxue Xuebao), 2008,66(13):1534-1540
29. [29]
  [29]SONG Wei(宋巍), CHEN Hong(陈红). Chin. Sci. Bull.(Kexue Tongbao), 2007,52(23):2701-2704
30. [30]
  [30]SHI Jie(史婕), FENG Bo(冯波), LU Xiong(鲁雄), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2011,26(12):1329-1303
31. [31]
  [31]LI De-Jun(李德军), YUAN Li(袁丽), YANG Ying(杨莹), et al. Sci. China. C(Zhongguo Kexue C), 2009,39(6):596-600
32. [32]
  [32]Doane T L, Chuang C H, Hill R J, et al. Acc. Chem. Res, 2012,45:317-326
33. [33]
  [33]Rabe M, Verdes D, Seeger S. Adv. Colloid. Interface. Sci, 2011,162:87-106
34. [34]
  [34]JIANG Yong(蒋勇), SONG Wu-Lin(宋武林), XIE Chang-Sheng(谢长生), et al. Rare Met. Mater. Eng.(Xiyou Jinshu Cailiao Yu Gongcheng), 2006,35(4):617-620
35. [35]
  [35]JIAO Zheng(焦正), CHEN Feng(陈锋), LI Min-Qiang(李民强), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2002,17 (2):316-320
36. [36]
  [36]SUN Mo-Jie(孙墨杰), HU Quan(胡全), LI Jian(李健), et al. Acta Chim. Sinica(Huaxue Xuebao), 2013,71:213-220
37. [37]
  [37]Xing Z, Ju Z, Yang J, et al. Nano Res., 2012,5(7):477-485
38. [38]
  [38]Barcena C, Sra A K, Chaubey G S, et al. Chem. Comm., 2008:2224-2226
39. [39]
  [39]Gao L, Wu J, Lyle S, et al. J. Phys. Chem. C, 2008,112: 17357-17361
40. [40]
  [40]Komarneni S, Fregeau E, Breval E, et al. J. Am. Ceram. Soc., 1988,71(1):C26-C28
41. [41]
  [41]SANG Shang-Bin(桑商斌), GU Ying-Ying(古映莹), HUANG Ke-Long(黄可龙). J. Funct. Mater.(Gongneng Cailiao), 2001, 32(1):27-29
42. [42]
  [42]Kumazawa H, Oki K, Cho H M, et al. Chem. Eng. Comm, 1992,115:25-33
43. [43]
  [43]Rezwan K, Studart A R, Voros J, et al. J. Phys. Chem. B, 2005,109:14469-14474
44. [44]
  [44]Liu X, Dai Q, Austin L, et al. J. Am. Chem. Soc., 2008,130: 2780-2782
45. [45]
  [45]Barth A. Biochim. Biophys. Acta, 2007,1767:1073-1101
46. [46]
  [46]Waldron R D. Phys. Rev., 1955,99:1727-1735
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Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins