Citation: LI Qiang, JI Xiao-Xu, ZHONG Qiu, HUANG Xin-Tang, XIONG Li. Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(11): 2375-2381. doi: 10.3969/j.issn.1001-4861.2013.00.368 shu

Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins

1.
1 Institute of Nanoscience and Nanotechnology, Central China Normal University, Wuhan 430079, China;

Received Date: 8 May 2013
Available Online: 20 June 2013
Fund Project: 国家自然科学基金(No.51172085)资助项目。 (No.51172085)

Ultrafine ZnFe₂O₄ nanocrystals were prepared by hydrothermal method and characterized by HRTEM, XRD and EDX techniques respectively. Protein adsorption properties and their correlations to ζ potentials between nanocrystals and protein molecules were investigated under different pH conditions using bovine serum albumin (BSA) and hemoglobin as model proteins. The hydrodynamic size of bare and protein loaded nanocrystals as well as protein conformation changes induced by nanocrystals were respectively studied by dynamic light scattering (DLS) and Fourier transform infrared (FTIR) spectroscopy techniques. Results show that adsorption between nanocrystals and hemoglobin obeys the law of electrostatistic interaction, whereas BSA adsorption behavior is not agreed with such law. After hemoglobin loading, most of nanocrystal-protein systems suspend as monomers and trimers apart from a few aggregates, whereas only aggregates exist after BSA adsorbing onto nanocrystals. FTIR spectroscopy revealed that Hemoglobin suffers more conformational changes than that of BSA. In addition, highly protein adsorption capacities exceeding₃₈₀ mg·g^-1 at appropriate pH conditions imply the potential applications of nanocrystals in protein separation.
- ZnFe₂O₄ nanocrystal;bovine serum albumin (BSA);hemoglobin;interactions
1. [1]
  [1] Mahmoudi M, Lynch I, Ejtehadi M R, et al. Chem. Rev., 2011,111:5610-5637
2. [2]
  [2] Nel A E, Mdler L, Velegol D, et al. Nat. Mater., 2009,8:543-557
3. [3]
  [3] Ge C C, Du J F, Zhao L, et al. PNAS, 2011,108:16968-16973
4. [4]
  [4] Hu W B, Peng C, Lü M, et al. ACS Nano, 2011,5:3693-3700
5. [5]
  [5] Sun S G, Ma M H, Qiu N, et al. Colloids. Surf. B Biointerfaces, 2011,88:246-253
6. [6]
  [6] DU Chong-Lei(杜崇磊), DU Wei(杜伟), WANG Bing(汪冰), et al. Chin. J. Anal. Chem.(Fenxi Huaxue), 2010,38(6):902-908
7. [7]
  [7] ZHAO Zi-Lai(赵紫来), BIAN Zheng-Yun(卞征云), CHEN Lang-Xing(陈朗星), et al. Prog. Chem.(Huaxue Jinzhan), 2006,18(10):1288-1297
8. [8]
  [8] HUANG Shan-Sheng(黄杉生), YIN Yue-Fen(殷月芬), WANG Ke-Min(王柯敏), et al. Chem. J. Chinese Universities (Gaodeng Xuexiao Huaxue Xuebao), 2004,25(12):2238-2241
9. [9]
  [9] HUANG Tian-Tian(黄天天), FU Yan(付雁), ZHANG Jin-Li (张金利), et al. Prog. Chem.(Huaxue Jinzhan), 2012,24(8): 1610-1622
10. [10]
  [10]ZHANG Huai(张怀), ZHANG Yun-Huai(张云怀), LI Jing (李静), et al. Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3): 253-259
11. [11]
  [11]NI Song-Bo(倪淞波), LI Yan-Bao(李延报), WANG Xiu-Mei (王秀梅). Prog. Chem.(Huaxue Jinzhan), 2011,23(1):231-245
12. [12]
  [12]LÜYa-Fen(吕亚芬), CAI Cheng-Xin(蔡称心). Acta Chim. Sinica(Huaxue Xuebao), 2006,64(24):2396-2402
13. [13]
  [13]ZOU Xue-Yan(邹雪艳), CHU Liu-Jie(褚留杰), DONG Shuo (董烁), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2012,33(7):1394-1400
14. [14]
  [14]LI Ya-Ru(李亚茹), ZHANG Xue-Lian(张雪莲), PAN Yuan-Yuan(潘园园), et al. J. Funct. Mater.(Gongneng Cailiao), 2012,43(8):1045-1048
15. [15]
  [15]WU Wei(吴伟), HE Quan-Guo(贺全国), CHEN Hong(陈洪). Prog. Chem.(Huaxue Jinzhan), 2008,20(2/3):265-272
16. [16]
  [16]Xu M, Li J, Iwai H, et al. Sci. Rep., 2012,2:1-6
17. [17]
  [17]YANG Xiao-Chao(杨小超), MO Zhi-Hong(莫志宏). Acta Chim. Sinica(Huaxue Xuebao), 2010,68(15):1549-1552
18. [18]
  [18]ZHANG Li(张莉), TANG Xin-Feng(唐新峰), GAO Wen-Bin (高文斌). J. Inorg. Mater.(Wuji Cailiao Xuebao), 2008,23(4): 860-864
19. [19]
  [19]YUAN Yuan(袁媛), HE Xiao-Xiao(何晓晓), SHI Hui(石慧), et al. Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(11):2167-2172
20. [20]
  [20]Gan X, Liu T, Zhong J, et al. Chem. Bio. Chem., 2004,5: 1686-1691
21. [21]
  [21]Zhou H, Gan X, Liu T, et al. J. Biochem. Biophys. Methods, 2005,64:38-45
22. [22]
  [22]Zhu X, Yuri I, Gan X, et al. Biosens. Bioelectron., 2007,22: 1600-1604
23. [23]
  [23]Zhou H, Gan X, Liu T, et al. Bioelectrochemistry, 2006,69: 34-40
24. [24]
  [24]SHAN Hong-Yan(单洪岩), LIU Dian-Jun(刘殿骏), WANG Zhen-Xin(王振新). Chem. J. Chinese Universities(Gaodeng Xuexiao Huaxue Xuebao), 2010,31(12):2372-2374
25. [25]
  [25]MENG Jie(孟洁), YANG Man(杨曼), WANG Chao-Ying(王超英), et al. New Carbon Mater.(Xinxing Tancailiao), 2007, 22(3):218-226
26. [26]
  [26]JU Si-Ting(鞠思婷), PU Ling-Yu(朴玲钰), YANG Lei(杨磊), et al. Prog. Chem.(Huaxue Jinzhan), 2010,22(9):1767-1775
27. [27]
  [27]GUO Xin-Lu(过馨露), ZHANG Jian(张建), WANG Wei(王炜). Prog. Phys.(Wulixue Jinzhan), 2012,32(6):285-293
28. [28]
  [28]TANG Shi-Hua(唐世华), HUANG Jian-Bin(黄建滨). Acta Chim. Sinica(Huaxue Xuebao), 2008,66(13):1534-1540
29. [29]
  [29]SONG Wei(宋巍), CHEN Hong(陈红). Chin. Sci. Bull.(Kexue Tongbao), 2007,52(23):2701-2704
30. [30]
  [30]SHI Jie(史婕), FENG Bo(冯波), LU Xiong(鲁雄), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2011,26(12):1329-1303
31. [31]
  [31]LI De-Jun(李德军), YUAN Li(袁丽), YANG Ying(杨莹), et al. Sci. China. C(Zhongguo Kexue C), 2009,39(6):596-600
32. [32]
  [32]Doane T L, Chuang C H, Hill R J, et al. Acc. Chem. Res, 2012,45:317-326
33. [33]
  [33]Rabe M, Verdes D, Seeger S. Adv. Colloid. Interface. Sci, 2011,162:87-106
34. [34]
  [34]JIANG Yong(蒋勇), SONG Wu-Lin(宋武林), XIE Chang-Sheng(谢长生), et al. Rare Met. Mater. Eng.(Xiyou Jinshu Cailiao Yu Gongcheng), 2006,35(4):617-620
35. [35]
  [35]JIAO Zheng(焦正), CHEN Feng(陈锋), LI Min-Qiang(李民强), et al. J. Inorg. Mater.(Wuji Cailiao Xuebao), 2002,17 (2):316-320
36. [36]
  [36]SUN Mo-Jie(孙墨杰), HU Quan(胡全), LI Jian(李健), et al. Acta Chim. Sinica(Huaxue Xuebao), 2013,71:213-220
37. [37]
  [37]Xing Z, Ju Z, Yang J, et al. Nano Res., 2012,5(7):477-485
38. [38]
  [38]Barcena C, Sra A K, Chaubey G S, et al. Chem. Comm., 2008:2224-2226
39. [39]
  [39]Gao L, Wu J, Lyle S, et al. J. Phys. Chem. C, 2008,112: 17357-17361
40. [40]
  [40]Komarneni S, Fregeau E, Breval E, et al. J. Am. Ceram. Soc., 1988,71(1):C26-C28
41. [41]
  [41]SANG Shang-Bin(桑商斌), GU Ying-Ying(古映莹), HUANG Ke-Long(黄可龙). J. Funct. Mater.(Gongneng Cailiao), 2001, 32(1):27-29
42. [42]
  [42]Kumazawa H, Oki K, Cho H M, et al. Chem. Eng. Comm, 1992,115:25-33
43. [43]
  [43]Rezwan K, Studart A R, Voros J, et al. J. Phys. Chem. B, 2005,109:14469-14474
44. [44]
  [44]Liu X, Dai Q, Austin L, et al. J. Am. Chem. Soc., 2008,130: 2780-2782
45. [45]
  [45]Barth A. Biochim. Biophys. Acta, 2007,1767:1073-1101
46. [46]
  [46]Waldron R D. Phys. Rev., 1955,99:1727-1735
1. [1]
  Chuanming GUO , Kaiyang ZHANG , Yun WU , Rui YAO , Qiang ZHAO , Jinping LI , Guang LIU . Performance of MnO₂-0.39IrO_x composite oxides for water oxidation reaction in acidic media. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1135-1142. doi: 10.11862/CJIC.20230459
2. [2]
  Jiaxun Wu , Mingde Li , Li Dang . The R eaction of Metal Selenium Complexes with Olefins as a Tutorial Case Study for Analyzing Molecular Orbital Interaction Modes. University Chemistry, 2025, 40(3): 108-115. doi: 10.12461/PKU.DXHX202405098
3. [3]
  Huiying Xu , Minghui Liang , Zhi Zhou , Hui Gao , Wei Yi . Application of Quantum Chemistry Computation and Visual Analysis in Teaching of Weak Interactions. University Chemistry, 2025, 40(3): 199-205. doi: 10.12461/PKU.DXHX202407011
4. [4]
  Hailian Tang , Siyuan Chen , Qiaoyun Liu , Guoyi Bai , Botao Qiao , Fei Liu . Stabilized Rh/hydroxyapatite Catalyst for Furfuryl Alcohol Hydrogenation: Application of Oxidative Strong Metal-Support Interactions in Reducing Conditions. Acta Physico-Chimica Sinica, 2025, 41(4): 100036-. doi: 10.3866/PKU.WHXB202408004
5. [5]
  Jingzhuo Tian , Chaohong Guan , Haobin Hu , Enzhou Liu , Dongyuan Yang . 废塑料促进S型NiCr₂O₄/孪晶Cd_0.5Zn_0.5S同质异质结光催化产氢. Acta Physico-Chimica Sinica, 2025, 41(6): 100068-. doi: 10.1016/j.actphy.2025.100068
6. [6]
  Jinwang Wu , Qijing Xie , Chengliang Zhang , Haifeng Shi . 自旋极化增强ZnFe_1.2Co_0.8O₄/BiVO₄ S型异质结光催化性能降解四环素. Acta Physico-Chimica Sinica, 2025, 41(5): 100050-. doi: 10.1016/j.actphy.2025.100050
7. [7]
  Yongjie ZHANG , Bintong HUANG , Yueming ZHAI . Research progress of formation mechanism and characterization techniques of protein corona on the surface of nanoparticles. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2318-2334. doi: 10.11862/CJIC.20240247
8. [8]
  Guoqiang Chen , Zixuan Zheng , Wei Zhong , Guohong Wang , Xinhe Wu . 熔融中间体运输导向合成富氨基g-C₃N₄纳米片用于高效光催化产H₂O₂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406021-. doi: 10.3866/PKU.WHXB202406021
9. [9]
  Endong YANG , Haoze TIAN , Ke ZHANG , Yongbing LOU . Efficient oxygen evolution reaction of CuCo₂O₄/NiFe-layered bimetallic hydroxide core-shell nanoflower sphere arrays. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 930-940. doi: 10.11862/CJIC.20230369
10. [10]
  Heng Chen , Longhui Nie , Kai Xu , Yiqiong Yang , Caihong Fang . 两步焙烧法制备大比表面积和结晶性增强超薄g-C₃N₄纳米片及其高效光催化产H₂O₂. Acta Physico-Chimica Sinica, 2024, 40(11): 2406019-. doi: 10.3866/PKU.WHXB202406019
11. [11]
  Siyu HOU , Weiyao LI , Jiadong LIU , Fei WANG , Wensi LIU , Jing YANG , Ying ZHANG . Preparation and catalytic performance of magnetic nano iron oxide by oxidation co-precipitation method. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1577-1582. doi: 10.11862/CJIC.20230469
12. [12]
  Hui Wang , Abdelkader Labidi , Menghan Ren , Feroz Shaik , Chuanyi Wang . 微观结构调控的g-C₃N₄在光催化NO转化中的最新进展：吸附/活化位点的关键作用. Acta Physico-Chimica Sinica, 2025, 41(5): 100039-. doi: 10.1016/j.actphy.2024.100039
13. [13]
  Shiyi WANG , Chaolong CHEN , Xiangjian KONG , Lansun ZHENG , Lasheng LONG . Polynuclear lanthanide compound [Ce₄^ⅢCe₆^Ⅳ(μ₃-O)₄(μ₄-O)₄(acac)₁₄(CH₃O)₆]·2CH₃OH for the hydroboration of amides to amine. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 88-96. doi: 10.11862/CJIC.20240342
14. [14]
  Fei ZHOU , Xiaolin JIA . Co₃O₄/TiO₂ composite photocatalyst: Preparation and synergistic degradation performance of toluene. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2232-2240. doi: 10.11862/CJIC.20240236
15. [15]
  Hailang JIA , Hongcheng LI , Pengcheng JI , Yang TENG , Mingyun GUAN . Preparation and performance of N-doped carbon nanotubes composite Co₃O₄ as oxygen reduction reaction electrocatalysts. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 693-700. doi: 10.11862/CJIC.20230402
16. [16]
  Dong-Xue Jiao , Hui-Li Zhang , Chao He , Si-Yu Chen , Ke Wang , Xiao-Han Zhang , Li Wei , Qi Wei . Layered (C5H6ON)2[Sb2O(C2O4)3] with a large birefringence derived from the uniform arrangement of π-conjugated units. Chinese Journal of Structural Chemistry, 2024, 43(6): 100304-100304. doi: 10.1016/j.cjsc.2024.100304
17. [17]
  Peng ZHOU , Xiao CAI , Qingxiang MA , Xu LIU . Effects of Cu doping on the structure and optical properties of Au₁₁(dppf)₄Cl₂ nanocluster. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1254-1260. doi: 10.11862/CJIC.20240047
18. [18]
  Qingtang ZHANG , Xiaoyu WU , Zheng WANG , Xiaomei WANG . Performance of nano Li₂FeSiO₄/C cathode material co-doped by potassium and chlorine ions. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1689-1696. doi: 10.11862/CJIC.20240115
19. [19]
  Xiuzheng Deng , Changhai Liu , Xiaotong Yan , Jingshan Fan , Qian Liang , Zhongyu Li . Carbon dots anchored NiAl-LDH@In₂O₃ hierarchical nanotubes for promoting selective CO₂ photoreduction into CH₄. Chinese Chemical Letters, 2024, 35(6): 108942-. doi: 10.1016/j.cclet.2023.108942
20. [20]
  Sikai Wu , Xuefei Wang , Huogen Yu . Hydroxyl-enriched hydrous tin dioxide-coated BiVO4 with boosted photocatalytic H2O2 production. Chinese Journal of Structural Chemistry, 2024, 43(12): 100457-100457. doi: 10.1016/j.cjsc.2024.100457

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(319)
HTML views(40)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Ultrafine ZnFe2O4 Nanocrystals Interacting with Proteins

Metrics

通讯作者: 陈斌, bchen63@163.com

Ultrafine ZnFe₂O₄ Nanocrystals Interacting with Proteins