Citation: ZHU Li-Na, WANG Hai-Xian, SUN Run-Feng, LI Ping, KONG De-Ming, LI Xiao-Zeng. Disruption of G-Quadruplex DNA by Ag⁺ and Hg²⁺ and Its Application to DNA Logic Gate[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(10): 2215-2224. doi: 10.3969/j.issn.1001-4861.2013.00.259 shu

Disruption of G-Quadruplex DNA by Ag⁺ and Hg²⁺ and Its Application to DNA Logic Gate

1.
1 Department of Chemistry, Tianjin University, Tianjin 300072, China;

Received Date: 1 March 2013
Available Online: 19 April 2013
Fund Project: 国家自然科学基金(No.20801040,21175072)资助项目 (No.20801040,21175072)

The G-quadruplex-disrupting abilities of Ag⁺ and Hg²⁺ ions were investigated by utilizing the metal ions-mediated circular dichroism (CD) spectrum changes of four representative G-quadruplexes. The results show that Ag⁺ ion can disrupt G-quadruplexes by chelating to Gbases and thus can be used as a general G-quadruplex-disrupting agent. The results also suggest that Hg²⁺ ion may disrupt G-quadruplexes by formation of T-Hg²⁺-Tbase pairs as well as other species. The G-quadruplexes disrupted by Ag⁺ and Hg²⁺ can be recovered by cysteine (Cys), a thiol-containing amino acid, due to the tight binding interactions between Cys and the metal ions. Thus, the system by using Ag⁺ (or Hg²⁺) and Cys as two inputs and CDsignal as output can behave as a DNA IMPLICATIONlogic gate.
- G-quadruplex; DNA logic gate; Ag⁺; Hg²⁺; cysteine; circular dichroism
1. [1]
  [1] Gilbert D E, Feigon J. Curr. Opin. Struct. Biol., 1999,9:305- 314 [2] Huppert J L. Chem. Soc. Rev., 2008,37:1375-1384 [3] Williamson J R. Annu. Rev. Biophys. Biomol. Struct., 1994, 23:703-730 [4] Rhodes D, Giraldo R. Curr. Opin. Struct. Biol., 1995,5:311- 322 [5] Zahler A M, Williamson J R, Cech T R, et al. Nature, 1991, 350:718-720 [6] Howell L A, Searcey M. ChemBioChem., 2009,10:2139-2143 [7] Shi S,Yao T M, Ji L N, et al. Dalton Trans., 2012,41:5789- 5793 [8] Shi S,Yao T M,Ji L N, et al. J. Inorg. Biochem., 2013,121:19 -27 [9] Lipps H J, Rhodes D. Trends Cell. Biol., 2009,19:414-422 [10]Neidle S. FEBS J., 2009,277:1118-1125 [11]Shalaby T, Hiyama E, Grotzer M A. Anticancer Agents Med. Chem., 2010,10:196-212 [12]Lechel A, Manns M P, Rudolph K L. J. Hepatol., 2004,41: 491-497 [13]Simonsson T. Biol. Chem., 2001,382:621-628 [14]Gill M L, Strobel S L, Loria J P. Nucleic Acids Res., 2006, 34:4506-4514 [15]Liu W, Liang H J, Fu Y. J. Phys. Chem. B, 2011,115:13051 -13056 [16]Li T, Dong S J, Wang E K. Anal. Chem., 2009,81:2144-2149 [17]Li T, Li B, Wang E K, et al. Chem. Commun., 2009,45: 3551-3553 [18]Zhou X H, Kong D M, Shen H X. Anal. Chem., 2010,82: 789-793 [19]Kong D M, Xu J, Shen H X. Anal. Chem., 2010,82:6148- 6153 [20]Topal S Z, Gürek A G, Ertekin K, et al. Mater. Chem. Phys., 2010,121:425-531 [21]Chen X, Wang Y, Liu Q, et al. Angew. Chem. Int. Ed. Engl., 2006,45:1759-1762 [22]Xie W Y, Huang W T, Li N B, et al. Chem. Commun., 2012,48:82-84 [23]Paramasivan S, Rujan I, Bolton P H. Methods, 2007,43:324- 331 [24]Kong D M, Cai L L, Guo J H, et al. Biopolymers, 2009,91: 331-339 [25]Monchaud D, Yang P, Lacroix L, et al. Angew. Chem. Int. Ed. Engl., 2008,47:4858-4861 [26]Ambrus A, Chen D, Dai J, et al. Nucleic Acids Res., 2006, 34:2723-2735 [27]Kong D M, Ma Y E, Guo J H, et al. Anal. Chem., 2009,81: 2678-2684 [28]Luu K N, Phan A T, Kuryavyi V, et al. J. Am. Chem. Soc., 2006,128:9963-9970 [29]Malumbres M, Barbacid M. Nat. Rev. Cancer, 2003,3:459- 465 [30]Xodo L, Paramasivam M, Membrino A, et al. Nucleic Acids Symp. Ser., 2008,52:159-160 [31]Chen Z, Zheng K W, Hao Y H, et al. J. Am. Chem. Soc., 2009,131:10430-10438 [32]Paramasivam M, Membrino A, Cogoi S, et al. Nucleic Acids Res., 2009,37:2841-2853 [33]Hotary K, Li X Y, Allen E, et al. Genes Dev., 2006,20:2673 -2686 [34]Morris M J, Basu S. Biochem., 2009,48:5313-5319 [35]Gruen L C. Biochim. Biophys. Acta, 1975,386:270-274 [36]Burstein Y, Sperling R. Biochim. Biophys. Acta, 1970,211: 410-412 [37]Guo J H, Kong D M, Shen H X. Biosens. Bioelectron., 2010,26:327-332 [38]Li T, Shi L L, Wang E K, et al. Chem. Eur. J., 2009,15: 3347-3350 [39]Ruan Y B, Li A F, Zhao J S, et al. Chem. Commun., 2010, 46:4938-4940 [40]Jia S M, Liu X F, Li P, et al. Biosens. Bioelectron., 2011, 27:148-152 [41]D'Urso A, Mammana A, Balaz M, et al. J. Am. Chem. Soc., 2009,131:2046-2047 [42]Zhou Y C, Zhang D Q, Zhang Y Z, et al. J. Org. Chem., 2005,70:6164-6170
1. [1]
  Yuhao SUN , Qingzhe DONG , Lei ZHAO , Xiaodan JIANG , Hailing GUO , Xianglong MENG , Yongmei GUO . Synthesis and antibacterial properties of silver-loaded sod-based zeolite. Chinese Journal of Inorganic Chemistry, 2024, 40(4): 761-770. doi: 10.11862/CJIC.20230169
2. [2]
  Yidan Jing , Xiaomin Zhang , Nan Xu . Design and Practice of Chemical Science Popularization Experiments Based on the Concept of Controlling Variables: Taking the “Recovery of Silver from Silver-Containing Wastewater” Science Popularization Project as an Example. University Chemistry, 2025, 40(4): 346-352. doi: 10.12461/PKU.DXHX202405146
3. [3]
  Wendian XIE , Yuehua LONG , Jianyang XIE , Liqun XING , Shixiong SHE , Yan YANG , Zhihao HUANG . Preparation and ion separation performance of oligoether chains enriched covalent organic framework membrane. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1528-1536. doi: 10.11862/CJIC.20240050
4. [4]
  Zhiming Feng , Lili Wu , Chengming Wang . Doubly Oxidized Carbene. University Chemistry, 2025, 40(9): 326-331. doi: 10.12461/PKU.DXHX202411008
5. [5]
  Yingran Liang , Fei Wang , Jiabao Sun , Hongtao Zheng , Zhenli Zhu . Construction and Application of a New Experimental Device for Determination of Alkaline Metal Elements by Plasma Atomic Emission Spectrometry Based on Solution Cathode Glow Discharge: An Alternative Approach for Fundamental Teaching Experiments in Emission Spectroscopy. University Chemistry, 2024, 39(5): 380-387. doi: 10.3866/PKU.DXHX202312024
6. [6]
  Jiaxuan Zuo , Kun Zhang , Jing Wang , Xifei Li . Nucleation Regulation and Mechanism of Precursors for Nickel Cobalt Manganese-based Cathode Materials in Lithium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(1): 100009-0. doi: 10.3866/PKU.WHXB202404042
7. [7]
  Xiaoning TANG , Shu XIA , Jie LEI , Xingfu YANG , Qiuyang LUO , Junnan LIU , An XUE . Fluorine-doped MnO₂ with oxygen vacancy for stabilizing Zn-ion batteries. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1671-1678. doi: 10.11862/CJIC.20240149
8. [8]
  Tianhao GE , Sirong LU , Zhiyin XIAO , Wei ZHONG . Synthesis of porphyrin-based ionic polymeric materials for catalytic application in CO₂ conversion. Chinese Journal of Inorganic Chemistry, 2026, 42(4): 722-736. doi: 10.11862/CJIC.20250312
9. [9]
  Hongyun Liu , Jiarun Li , Xinyi Li , Zhe Liu , Jiaxuan Li , Cong Xiao . Course Ideological and Political Design of a Comprehensive Chemistry Experiment: Constructing a Visual Molecular Logic System Based on Intelligent Hydrogel Film Electrodes. University Chemistry, 2024, 39(2): 227-233. doi: 10.3866/PKU.DXHX202309070
10. [10]
  Junyang FENG , Xiaoli HAN , Yongjie SONG , Gang LI . Proton conduction and fluorescence properties of an ionic hydrogen-bonded organic framework constructed from dibromophthalic acid. Chinese Journal of Inorganic Chemistry, 2026, 42(4): 693-702. doi: 10.11862/CJIC.20250350
11. [11]
  Zhipeng Bao , Yilin Wang , Yu Chen , Beirui Jia , Congcong Wang , Zean Xie , Xuehua Yu , Zhen Zhao . Digital and Intelligent Integration under the “Dual Carbon” Strategy: Plasma Reaction-Separation Coupling for CO₂ Hydrogenation to Methanol. University Chemistry, 2026, 41(1): 29-40. doi: 10.12461/PKU.DXHX202506009
12. [12]
  Kezhen Qi , Bei Cheng , Kaiqiang Xu . Ultrafast interfacial charge transfer promoted by the LSPR of Au nanoparticles for photocatalytic H₂ evolution. Acta Physico-Chimica Sinica, 2026, 42(3): 100205-0. doi: 10.1016/j.actphy.2025.100205
13. [13]
  Yujin Deng , Yishuang Chen , Lijie Zhang , Huile Jin , Yun Yang , Quanlong Xu , Shun Wang . Plasmonic Au nanobipyramid assembly covalent organic framework for boosting photocatalytic hydrogen evolution through strong local electric field. Acta Physico-Chimica Sinica, 2026, 42(6): 100193-0. doi: 10.1016/j.actphy.2025.100193
14. [14]
  Ting YANG , Jia AN , Jinyu ZHANG , Ruonan FAN , Rong YAN , Xiaoxia JING , Panpan CHANG , Wei YAN . Synergistic enhancement of ion migration and sulfur conversion kinetics in lithium-sulfur batteries by CeO₂/g-C₃N₄. Chinese Journal of Inorganic Chemistry, 2026, 42(3): 519-530. doi: 10.11862/CJIC.20250274
15. [15]
  Yuyao Wang , Zhitao Cao , Zeyu Du , Xinxin Cao , Shuquan Liang . Research Progress of Iron-based Polyanionic Cathode Materials for Sodium-Ion Batteries. Acta Physico-Chimica Sinica, 2025, 41(4): 100035-0. doi: 10.3866/PKU.WHXB202406014
16. [16]
  Wang Shuang , Fu Xiaoqi , Yao Shanshan . Synergistic optimization of ion migration and electron transfer in sodium-ion battery cathode materials. Acta Physico-Chimica Sinica, 2026, 42(5): 100206-. doi: 10.1016/j.actphy.2025.100206
17. [17]
  Liuyun Chen , Wenju Wang , Tairong Lu , Xuan Luo , Xinling Xie , Kelin Huang , Shanli Qin , Tongming Su , Zuzeng Qin , Hongbing Ji . Soft template-induced deep pore structure of Cu/Al₂O₃ for promoting plasma-catalyzed CO₂ hydrogenation to DME. Acta Physico-Chimica Sinica, 2025, 41(6): 100054-0. doi: 10.1016/j.actphy.2025.100054
18. [18]
  Wenjun Zheng . Application in Inorganic Synthesis of Ionic Liquids. University Chemistry, 2024, 39(8): 163-168. doi: 10.3866/PKU.DXHX202401020
19. [19]
  Guoze Yan , Bin Zuo , Shaoqing Liu , Tao Wang , Ruoyu Wang , Jinyang Bao , Zhongzhou Zhao , Feifei Chu , Zhengtong Li , Yamauchi Yusuke , Melhi Saad , Xingtao Xu . Opportunities and Challenges of Capacitive Deionization for Uranium Extraction from Seawater. Acta Physico-Chimica Sinica, 2025, 41(4): 100032-0. doi: 10.3866/PKU.WHXB202404006
20. [20]
  Jing Tan , Bo Zheng , Lingyan Gao . Application of Crown Ether-Based Artificial Ion Transporters. University Chemistry, 2026, 41(2): 232-237. doi: 10.12461/PKU.DXHX202501010

Get Citation

PDF

XML

Metrics

PDF Downloads(0)
Abstract views(815)
HTML views(41)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

Disruption of G-Quadruplex DNA by Ag+ and Hg2+ and Its Application to DNA Logic Gate

Metrics

通讯作者: 陈斌, bchen63@163.com

Disruption of G-Quadruplex DNA by Ag⁺ and Hg²⁺ and Its Application to DNA Logic Gate