Citation: GUO Ming, HUANG Feng-Qin, LIU Mi-Mi, LI Ming-Hui. Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(5): 1037-1044. doi: 10.3969/j.issn.1001-4861.2013.00.159 shu

Performance of Binding Interaction between Cr(Ⅵ) and Serum Proteome

1.
Department of Chemistry, Zhejiang Agriculture & Forestry University, Lin'an, Zhejiang 311300, China

Corresponding author: GUO Ming,
Received Date: 25 December 2012
Available Online: 31 January 2013
Fund Project: 国家自然科学基金(No.20877072) (No.20877072)浙江科技厅公益项目(No.2012C37055) 资助项目。 (No.2012C37055)

The binding reaction between heavy metal ions Cr(Ⅵ) and serum proteome was investigated using affinity capillary electrophoresis(ACE) method. Based on the site binding model, a model about the interaction between Cr(Ⅵ) as the ligand and FBS as the receptor, was established. The binding performance of heavy metal ions Cr(Ⅵ) and the serum proteome was studied under proteomic conditions, and the results showed that, Cr(Ⅵ)-protein complexes formed between Cr(Ⅵ) and the high abundance component albumin in FBS, such as albumin (bovine serum albumin, BSA), transferrin protein (Bovine apo transferrin, bATF), immunogolobulin (Bovine IgG, IgG) and so on, and the binding reactions of Cr(Ⅵ) -FBS are fast equilibrium reactions. The apparent binding constant was determined through non-linear fitting binding reaction and based on changes of the effective mobility, and K_Cr(Ⅵ)-bATF=1.27×10⁴ L·mol^-1, K_Cr(Ⅵ)-IgG=4.91×10⁴ L·mol^-1, K_Cr(Ⅵ)-BSA=8.98×10⁴ L·mol^-1. Dosage-dependent effect exists among the concentration of Cr(Ⅵ) and the strength of the binding reactions. This work has referential meaning for understanding deeply the binding reaction mechanism of chromium(Ⅵ) with serum proteome.
- Cr(Ⅵ);serum proteome;apparent binding constant;complex;dosage-dependent effect
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