Citation: TANG Jing-Long, WANG Shuo, LIU Li, WANG Chun-Ren, XI Ting-Fei. A Preliminary Study on the Dose-Effect Relation when Silver Nanoparticles Crossing through the Blood-Brain Barrier in vitro[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(5): 1025-1030. doi: 10.3969/j.issn.1001-4861.2013.00.150 shu

A Preliminary Study on the Dose-Effect Relation when Silver Nanoparticles Crossing through the Blood-Brain Barrier in vitro

1.
1. National Institutes for Food and Drug Control, Beijing 100050, China;

Corresponding author: TANG Jing-Long,
Received Date: 25 December 2012
Available Online: 21 January 2013
Fund Project: 北京市自然科学基金(No.3112024) (No.3112024)中国食品药品检定研究院中青年发展研究基金(No.2009C4)资助项目。 (No.2009C4)

Objective:The aim of this study was to investigate the dose-effect relation of the ability that silver nanoparticles (SNPs) crossing through the blood-brain barrier (BBB) and presume its mechanism. Methods:In this study, SNPs were test samples and silver microparticles (SMPs) acted as control samples. First, an in vitro BBB model was established. Second, SNPs or SMPs were cultured in the BBB model at different dose from 25 μg·mL^-1 to 400 μg·mL^-1, respectively. After 4 hours of culture, the ultrastructure of BBB and the percentage of silver particles crossing through BBB were evaluated with TEM and ICP-MS respectively. Results:Results demonstrated that SNPs crossed the BBB, while the SMPs did not. When SNPS dose<100 μg·mL^-1, about 2% silver particles crossed BBB, and the BBBs ultrastructures were normal. When dose>100 μg·mL^-1, the higher dose is, the more silver particles crossed BBB, and the more damage occurred in the BBBs ultrastructures. When dose=400 μg·mL^-1, about 15% silver particles crossed BBB. Conclusion:The results suggested that membrane-mobile transport mechanism is the main way allowing SNPs to cross the BBB in low dose. Cytotoxicity mechanism is the main way allowing SNPs to cross the BBB in higher dose. It is also suggested that SNPs could cross the BBB even in a low dose. A cautious attitude would be hold before silver nanoparticles-based medical devices were used in clinical practice.
- silver nanoparticle;blood-brain barrier;dose-effect relation
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