Citation: WANG Lei, CHEN Cheng, TIAN Mei-Juan, YANG Yu-Xiang, LUO Jian-Hui, DING Bin, WANG Ping-Mei. Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118 shu

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

1.
1 Department of Chemistry, East China University of Science and Techology, Shanghai 200237, China;

Received Date: 4 September 2012
Available Online: 12 December 2012
Fund Project: 中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目。 (2011A-1001)

Mesoporous silica was prepared by using hexadecyl trimethyl ammonium bromide (CTAB) as the template, tetraethoxysilane (tetraethylorthosilicate, TEOS) as the silicon source and nitric acid as the hydrolysis catalyst. The obtained sample was then modified via post grafting method. The mesoporous silica before and after modification was characterized by IR, DTA-TG, XRD, SEM, elemental analysis, micro-electrophoresis and N₂ adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.
- mesoporous silica;post-synthesis grafting method;modification;enzymes;immobilization
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