Citation: WANG Lei, CHEN Cheng, TIAN Mei-Juan, YANG Yu-Xiang, LUO Jian-Hui, DING Bin, WANG Ping-Mei. Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118 shu

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

1.
1 Department of Chemistry, East China University of Science and Techology, Shanghai 200237, China;

Received Date: 4 September 2012
Available Online: 12 December 2012
Fund Project: 中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目。 (2011A-1001)

Mesoporous silica was prepared by using hexadecyl trimethyl ammonium bromide (CTAB) as the template, tetraethoxysilane (tetraethylorthosilicate, TEOS) as the silicon source and nitric acid as the hydrolysis catalyst. The obtained sample was then modified via post grafting method. The mesoporous silica before and after modification was characterized by IR, DTA-TG, XRD, SEM, elemental analysis, micro-electrophoresis and N₂ adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.
- mesoporous silica;post-synthesis grafting method;modification;enzymes;immobilization
1. [1]
  [1] Zhang X, Guan R F, Wu D Q, et al. J. Mol. Catal. B: Enzym., 2005,33:43-50
2. [2]
  [2] Zhao X S, Lu G Q, Whittaker A K, et al. J. Phys. Chem. B, 1997,101(33):6525-6531
3. [3]
  [3] Jal P K, Patel S, Mishra B K. Talanta, 2004,62:1005-1028
4. [4]
  [4] Yiu H H P, Wright P A. J. Mater. Chem., 2005,15:3690-3700
5. [5]
  [5] Deere J, Magner E, Wall J G, et al. Chem. Commun., 2001, 5:465-466
6. [6]
  [6] Yokoi T, Yoshitake H, Tatsumi T. J. Mater. Chem., 2004,14: 951-957
7. [7]
  [7] Hakulinen N, Kiiskinen L L, Kruus K, et al. Nat. Struct. Boil., 2002,9(8):601-605
8. [8]
  [8] Zhu Y F, Kaskel S, Shi J L, et al. Chem. Mater., 2007,19: 6408-6413
9. [9]
  [9] Osiadacz J, Al-Adhami A J H, Bajraszewska D, et al. J. Biotechnol., 1999,72:141-149
10. [10]
  [10] Lei J, Fan J, Yu C, et al. Microporous Mesoporous Mater., 2004,73:121-128
11. [11]
  [11] WANG Zhi-Yin(汪志银), DING Hai-Jun(丁海军), YANG Yu-Xiang(杨宇翔), et al. Chinese J. Inorg. Chem.(Wuji Huaxue Xuebao), 2011,27(3):409-420
12. [12]
  [12] MA Qian(马骞), MAO Xue-Feng(毛学峰), WEI Hong-Guang (魏宏广), et al. J. Northwest Normal Univ. Natural Science (Xibei Shifan Daxue Xuebao: Ziran Kexue Ban), 2003,39(4): 54-56
13. [13]
  [13] Han D, Jia W, Liang H. J. Environ. Sciences, 2010,22(2): 237-241
14. [14]
  [14] YUAN Peng (袁鹏), WU Da-Qing(吴大清), CHEN Zhong(陈忠), et al. Chinese Science Bull.(Kexue Tongbao), 2001,46 (4):342-344
15. [15]
  [15] JIANG De-Sheng(姜德生), LONG Sheng-Ya(龙胜亚), HUANG Jun (黄俊), et al. Acta Microbiol. Sinica(Wei Shengwu Xuebao), 2005,45(4):630-633
16. [16]
  [16] Ayhan F, Dogac Y I, Ayhan H, et al. Hacettepe J. Biol. Chem., 2011,39(3):241-251
17. [17]
  [17] Bollag J M, Shuttleworth K L, Anderson D H. Appl. Environ. Microbio., 1988,54(12):3086-3091
18. [18]
  [18] YANG Yu-Xiang(杨宇翔), WANG Peng(王鹏), WU Jie-Da (吴介达), et al. Chinese J. Inorg. Chem.(Wuji Huaxue Xuebao), 1998,14(1):47-52
19. [19]
  [19] Yang Y X, Ying H P, Shao J G, et al. J. Am. Ceram. Soc., 2007,90(11):3460-3467
20. [20]
  [20] Yang Y X, Huang Z, Deng W J, et al. Microporous Mesoporous Mater., 2008,116:267-276
21. [21]
  [21] Yadav G D, Jadhav S R. Microporous Mesoporous Mater., 2005,86:215-222
22. [22]
  [22] Zimmerman A R, Goyne K W, Chorover J, et al. Org. Geochem., 2004,35:335-375
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沈阳化工大学材料科学与工程学院沈阳 110142