Citation: WANG Lei, CHEN Cheng, TIAN Mei-Juan, YANG Yu-Xiang, LUO Jian-Hui, DING Bin, WANG Ping-Mei. Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase[J]. Chinese Journal of Inorganic Chemistry, ;2013, 29(4): 677-688. doi: 10.3969/j.issn.1001-4861.2013.00.118 shu

Modified Mesoporous Silica: Synthesis and Immobilization Function for Novozymes Laccase

1.
1 Department of Chemistry, East China University of Science and Techology, Shanghai 200237, China;

Received Date: 4 September 2012
Available Online: 12 December 2012
Fund Project: 中国石油天然气集团公司科学研究与技术开发项目《纳米级智能化学驱油剂研制》(2011A-1001)资助项目。 (2011A-1001)

Mesoporous silica was prepared by using hexadecyl trimethyl ammonium bromide (CTAB) as the template, tetraethoxysilane (tetraethylorthosilicate, TEOS) as the silicon source and nitric acid as the hydrolysis catalyst. The obtained sample was then modified via post grafting method. The mesoporous silica before and after modification was characterized by IR, DTA-TG, XRD, SEM, elemental analysis, micro-electrophoresis and N₂ adsorption-desorption. The results show that the mesoporous silica has been modified by amino-groups, the material after modification still belongs to a family of mesoporous materials in spite of a slight decrease in the order degree. The surface area, pore size and pore volume become smaller, and the isoelectric point is decreased from 2.74 to 4.75 after the modification. The commercial Novozymes laccase is successfully immobilized in the inner surface of amino-functionalized mesoporous silica in this work by using glutaraldehyde as the crosslinking agent. The conditions of immobilization on amino-functionalized mesoporous silica were optimized by means of orthogonal experimental designs. The results show that the operational stability of the immobilized enzyme is significantly improved in comparison with the free enzyme.
- mesoporous silica;post-synthesis grafting method;modification;enzymes;immobilization
1. [1]
  [1] Zhang X, Guan R F, Wu D Q, et al. J. Mol. Catal. B: Enzym., 2005,33:43-50
2. [2]
  [2] Zhao X S, Lu G Q, Whittaker A K, et al. J. Phys. Chem. B, 1997,101(33):6525-6531
3. [3]
  [3] Jal P K, Patel S, Mishra B K. Talanta, 2004,62:1005-1028
4. [4]
  [4] Yiu H H P, Wright P A. J. Mater. Chem., 2005,15:3690-3700
5. [5]
  [5] Deere J, Magner E, Wall J G, et al. Chem. Commun., 2001, 5:465-466
6. [6]
  [6] Yokoi T, Yoshitake H, Tatsumi T. J. Mater. Chem., 2004,14: 951-957
7. [7]
  [7] Hakulinen N, Kiiskinen L L, Kruus K, et al. Nat. Struct. Boil., 2002,9(8):601-605
8. [8]
  [8] Zhu Y F, Kaskel S, Shi J L, et al. Chem. Mater., 2007,19: 6408-6413
9. [9]
  [9] Osiadacz J, Al-Adhami A J H, Bajraszewska D, et al. J. Biotechnol., 1999,72:141-149
10. [10]
  [10] Lei J, Fan J, Yu C, et al. Microporous Mesoporous Mater., 2004,73:121-128
11. [11]
  [11] WANG Zhi-Yin(汪志银), DING Hai-Jun(丁海军), YANG Yu-Xiang(杨宇翔), et al. Chinese J. Inorg. Chem.(Wuji Huaxue Xuebao), 2011,27(3):409-420
12. [12]
  [12] MA Qian(马骞), MAO Xue-Feng(毛学峰), WEI Hong-Guang (魏宏广), et al. J. Northwest Normal Univ. Natural Science (Xibei Shifan Daxue Xuebao: Ziran Kexue Ban), 2003,39(4): 54-56
13. [13]
  [13] Han D, Jia W, Liang H. J. Environ. Sciences, 2010,22(2): 237-241
14. [14]
  [14] YUAN Peng (袁鹏), WU Da-Qing(吴大清), CHEN Zhong(陈忠), et al. Chinese Science Bull.(Kexue Tongbao), 2001,46 (4):342-344
15. [15]
  [15] JIANG De-Sheng(姜德生), LONG Sheng-Ya(龙胜亚), HUANG Jun (黄俊), et al. Acta Microbiol. Sinica(Wei Shengwu Xuebao), 2005,45(4):630-633
16. [16]
  [16] Ayhan F, Dogac Y I, Ayhan H, et al. Hacettepe J. Biol. Chem., 2011,39(3):241-251
17. [17]
  [17] Bollag J M, Shuttleworth K L, Anderson D H. Appl. Environ. Microbio., 1988,54(12):3086-3091
18. [18]
  [18] YANG Yu-Xiang(杨宇翔), WANG Peng(王鹏), WU Jie-Da (吴介达), et al. Chinese J. Inorg. Chem.(Wuji Huaxue Xuebao), 1998,14(1):47-52
19. [19]
  [19] Yang Y X, Ying H P, Shao J G, et al. J. Am. Ceram. Soc., 2007,90(11):3460-3467
20. [20]
  [20] Yang Y X, Huang Z, Deng W J, et al. Microporous Mesoporous Mater., 2008,116:267-276
21. [21]
  [21] Yadav G D, Jadhav S R. Microporous Mesoporous Mater., 2005,86:215-222
22. [22]
  [22] Zimmerman A R, Goyne K W, Chorover J, et al. Org. Geochem., 2004,35:335-375
1. [1]
  Xuejiao Wang , Suiying Dong , Kezhen Qi , Vadim Popkov , Xianglin Xiang . Photocatalytic CO₂ Reduction by Modified g-C₃N₄. Acta Physico-Chimica Sinica, 2024, 40(12): 2408005-. doi: 10.3866/PKU.WHXB202408005
2. [2]
  Xiaosong PU , Hangkai WU , Taohong LI , Huijuan LI , Shouqing LIU , Yuanbo HUANG , Xuemei LI . Adsorption performance and removal mechanism of Cd(Ⅱ) in water by magnesium modified carbon foam. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1537-1548. doi: 10.11862/CJIC.20240030
3. [3]
  Fan Wu , Wenchang Tian , Jin Liu , Qiuting Zhang , YanHui Zhong , Zian Lin . Core-Shell Structured Covalent Organic Framework-Coated Silica Microspheres as Mixed-Mode Stationary Phase for High Performance Liquid Chromatography. University Chemistry, 2024, 39(11): 319-326. doi: 10.12461/PKU.DXHX202403031
4. [4]
  Xinpeng LIU , Liuyang ZHAO , Hongyi LI , Yatu CHEN , Aimin WU , Aikui LI , Hao HUANG . Ga₂O₃ coated modification and electrochemical performance of Li_1.2Mn_0.54Ni_0.13Co_0.13O₂ cathode material. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1105-1113. doi: 10.11862/CJIC.20230488
5. [5]
  Quanliang Chen , Zhaohui Zhou . Research on the Active Site of Nitrogenase over Fifty Years. University Chemistry, 2024, 39(7): 287-293. doi: 10.3866/PKU.DXHX202310133
6. [6]
  Yifeng TAN , Ping CAO , Kai MA , Jingtong LI , Yuheng WANG . Synthesis of pentaerythritol tetra(2-ethylthylhexoate) catalyzed by h-MoO₃/SiO₂. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2155-2162. doi: 10.11862/CJIC.20240147
7. [7]
  Guang-Xu Duan , Queting Chen , Rui-Rui Shao , Hui-Huang Sun , Tong Yuan , Dong-Hao Zhang . Encapsulating lipase on the surface of magnetic ZIF-8 nanosphers with mesoporous SiO₂ nano-membrane for enhancing catalytic performance. Chinese Chemical Letters, 2025, 36(2): 109751-. doi: 10.1016/j.cclet.2024.109751
8. [8]
  Hongyi LI , Aimin WU , Liuyang ZHAO , Xinpeng LIU , Fengqin CHEN , Aikui LI , Hao HUANG . Effect of Y(PO₃)₃ double-coating modification on the electrochemical properties of Li[Ni_0.8Co_0.15Al_0.05]O₂. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1320-1328. doi: 10.11862/CJIC.20230480
9. [9]
  Bing LIU , Huang ZHANG , Hongliang HAN , Changwen HU , Yinglei ZHANG . Visible light degradation of methylene blue from water by triangle Au@TiO₂ mesoporous catalyst. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 941-952. doi: 10.11862/CJIC.20230398
10. [10]
  Xinpin Pan , Yongjian Cui , Zhe Wang , Bowen Li , Hailong Wang , Jian Hao , Feng Li , Jing Li . Robust chemo-mechanical stability of additives-free SiO₂ anode realized by honeycomb nanolattice for high performance Li-ion batteries. Chinese Chemical Letters, 2024, 35(10): 109567-. doi: 10.1016/j.cclet.2024.109567
11. [11]
  Wei HE , Jing XI , Tianpei HE , Na CHEN , Quan YUAN . Application of solar-driven inorganic semiconductor-microbe hybrids in carbon dioxide fixation and biomanufacturing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 35-44. doi: 10.11862/CJIC.20240364
12. [12]
  Heng Zhang . Determination of All Rate Constants in the Enzyme Catalyzed Reactions Based on Michaelis-Menten Mechanism. University Chemistry, 2024, 39(4): 395-400. doi: 10.3866/PKU.DXHX202310047
13. [13]
  Chunmei GUO , Weihan YIN , Jingyi SHI , Jianhang ZHAO , Ying CHEN , Quli FAN . Facile construction and peroxidase-like activity of single-atom platinum nanozyme. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1633-1639. doi: 10.11862/CJIC.20240162
14. [14]
  Limei CHEN , Mengfei ZHAO , Lin CHEN , Ding LI , Wei LI , Weiye HAN , Hongbin WANG . Preparation and performance of paraffin/alkali modified diatomite/expanded graphite composite phase change thermal storage material. Chinese Journal of Inorganic Chemistry, 2024, 40(3): 533-543. doi: 10.11862/CJIC.20230312
15. [15]
  Bao Jia , Yunzhe Ke , Shiyue Sun , Dongxue Yu , Ying Liu , Shuaishuai Ding . Innovative Experimental Teaching for the Preparation and Modification of Conductive Organic Polymer Thin Films in Undergraduate Courses. University Chemistry, 2024, 39(10): 271-282. doi: 10.12461/PKU.DXHX202404121
16. [16]
  Xiaotian ZHU , Fangding HUANG , Wenchang ZHU , Jianqing ZHAO . Layered oxide cathode for sodium-ion batteries: Surface and interface modification and suppressed gas generation effect. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 254-266. doi: 10.11862/CJIC.20240260
17. [17]
  Liwei Wang , Guangran Ma , Li Wang , Fugang Xu . A Comprehensive Analytical Chemistry Experiment: Colorimetric Detection of Vitamin C Using Nanozyme and Smartphone. University Chemistry, 2024, 39(8): 255-262. doi: 10.3866/PKU.DXHX202312094
18. [18]
  Zhuoya WANG , Le HE , Zhiquan LIN , Yingxi WANG , Ling LI . Multifunctional nanozyme Prussian blue modified copper peroxide: Synthesis and photothermal enhanced catalytic therapy of self-provided hydrogen peroxide. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2445-2454. doi: 10.11862/CJIC.20240194
19. [19]
  Yuanpei ZHANG , Jiahong WANG , Jinming HUANG , Zhi HU . Preparation of magnetic mesoporous carbon loaded nano zero-valent iron for removal of Cr(Ⅲ) organic complexes from high-salt wastewater. Chinese Journal of Inorganic Chemistry, 2024, 40(9): 1731-1742. doi: 10.11862/CJIC.20240077
20. [20]
  Junke LIU , Kungui ZHENG , Wenjing SUN , Gaoyang BAI , Guodong BAI , Zuwei YIN , Yao ZHOU , Juntao LI . Preparation of modified high-nickel layered cathode with LiAlO₂/cyclopolyacrylonitrile dual-functional coating. Chinese Journal of Inorganic Chemistry, 2024, 40(8): 1461-1473. doi: 10.11862/CJIC.20240189

Get Citation

PDF

XML

Metrics

PDF Downloads(237)
Abstract views(770)
HTML views(86)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142