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Citation: AN Lingling, LU Maofeng, DENG Lingling, DU Jiangyan. Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage[J]. Chinese Journal of Applied Chemistry, ;2014, 31(2): 200-205. doi: 10.3724/SP.J.1095.2014.30179 shu

Electrochemical Activities of 8-Hydroxydeoxyguanosine in Chitosan/Graphene Modified Glassy Carbon Electrode and Detection of DNA Oxidative Damage

AN Lingling ^a ,
LU Maofeng ^a ,
DENG Lingling ^a ,
DU Jiangyan ^a,b,c,,

1.
a College of Chemistry and Materials Science, Nanjing Normal University;
2.
b Jiangsu Key Laboratory of Bio-functional Material;
3.
c Jiangsu Key Laboratory of New Power Batteries, Nanjing 210046, China

Corresponding author: DU Jiangyan,
Received Date: 12 April 2013
Available Online: 17 June 2013
Fund Project: 江苏高校优势学科建设工程资助项目(164320H106) (164320H106)

The electrochemical behavior of 8-hydroxydeoxyguanosine(8-OHdG) at the chitosan(Chi)/graphene(GR) modified glassy carbon electrode(GCE) was investigated by cyclic voltammetry,linear sweep voltammetry and differential pulse voltammetry.A linear relationship between the oxidative peak current and concentrations of 8-OHdG at the modified electrode was established in the range of 3.5×10^-7~1.4×10^-4 mol/L with a detection limit of 6.4×10^-8 mol/L(S/N=3).Chi/GR/GCE was used for the detection of DNA oxidative damage,it showed that the oxidative peak current of 8-OHdG at the modified electrode was linear with concentration of damaged DNA in the range of 10~300 mg/L with a detection limit of 0.026 mg/L(S/N=3).
- hydroxydeoxyguanosine,
- chitosan,
- graphene,
- DNA oxidative damage,
- electrochemical detection
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