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Citation: TANG Ronghu, DUAN Wei, CHEN Bo. Preparation of Genipin Cross-linked Magnetic Chitosan Microsphere and Its Immobilization on Lipase[J]. Chinese Journal of Applied Chemistry, ;2013, 30(8): 922-926. doi: 10.3724/SP.J.1095.2013.20485 shu

Preparation of Genipin Cross-linked Magnetic Chitosan Microsphere and Its Immobilization on Lipase

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Minwastry of Education, Hunan Normal University, Changsha 410081, China

Received Date: 24 October 2012
Revised Date: 11 December 2012

Inverse suspension combined with the sol-gel method was deployed to prepare a magnetic chitosan microspheres. The lipase was immobilized on magnetic chitosan microspheres with genipin as the cross-linking reagent. The optimum conditions and properties of immobilization enzyme were investigated. The maximal specific activity of 4.31 U/g was achieved when the crosslinking reaction was carried out at 55℃ for 8 h using 0.6 g/L genipin. The optimum pH,temperature and K_m of the immobilized enzyme were 8,25~35℃ and 0.26 mol/L,respectively. The immobilized lipase showed improved thermal stability and remained active in a broader pH range,compared with those of free lipase. Such immobilized lipase can be recycled via the magnetic separation for a certain extent.
- magnetic chitosan microspheres,
- genipin,
- immobilized enzyme
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