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Citation: LI Li, TANG Yongzheng, JIANG Yuanyuan, LU Tianhong, YANG Xujie, YANG Xiaodi. Adsorption Behavior of Nicotinamide Adenine Dinucleotide on α-Aluminum Oxide Nanoparticles[J]. Chinese Journal of Applied Chemistry, ;2012, 29(5): 551-556. doi: 10.3724/SP.J.1095.2012.00276 shu

Adsorption Behavior of Nicotinamide Adenine Dinucleotide on α-Aluminum Oxide Nanoparticles

LI Li ^a,c ,
TANG Yongzheng ^a ,
JIANG Yuanyuan ^b ,
LU Tianhong ^b ,
YANG Xujie ^a ,
YANG Xiaodi ^a,b,,

1.
a Key Laboratory for Soft Chemistry and Functional Materials of Ministry of Education, School of Chemical Engineering, Nanjing University of Science and Technology, Nanjing 210094, China;
2.
b Jiangsu Key Laboratory of New Power Batteries, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210097, China;
3.
c School of Biochemical and Environmental Engineering, Nanjing Xiaozhuang University, Nanjing 211171, China

Corresponding author: YANG Xiaodi,
Received Date: 24 June 2011
Available Online: 30 August 2011
Fund Project: 国家自然科学基金(20875047,20873065)重点基金(20833006) (20875047,20873065)重点基金(20833006)

The adsorption behavior of nicotinamide adenine dinucleotide(NAD⁺) on α-Al₂O₃ nanoparticles was studied using ultraviolet-visible(UV-Vis) absorption spectroscopy,X-ray diffraction(XRD),fluorescence and attenuated total reflectance-Fourier transform infrared(ATR-FTIR) spectroscopy.It was found that the amount of adsorbed NAD⁺ exhibited a strong dependence on pH and ionic strength of the solution,which suggested that the adsorption of NAD⁺ at α-Al₂O₃ nanoparticles was caused predominantly by the electrostatic adsorption.ATR-FTIR spectra of adsorbed NAD⁺ were similar to those of NAD⁺ in solution.However,the position of peaks for phosphate moving to slightly higher wavenumbers suggested that phosphate participated into the electrostatic adsorption.The adsorption isotherm could be fitted with both Langmuir and Freundlich isotherms.The fluorescence spectral features showed that the conformation of NAD⁺ changed with the change of the amount of α-Al₂O₃ nanoparticles.
- adsorption,
- nicotinamide adenine dinucleotide,
- electrostatic interaction,
- conformation
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