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Citation: CAO Huihui, PENG Jingdong, ZHANG Lei. Quantitative Determination of Bezafibrate in Rat Plasma Using HPLC-MS[J]. Chinese Journal of Applied Chemistry, ;2012, 29(5): 591-596. doi: 10.3724/SP.J.1095.2012.00269 shu

Quantitative Determination of Bezafibrate in Rat Plasma Using HPLC-MS

1.
Chemistry and Chemical Engineering, Southwest University, Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, Chongqing 400715

Received Date: 21 June 2011
Available Online: 15 August 2011

A simple,rapid and sensitive HPLC-MS method was developed and validated for determining bezafibrate in rat plasma.Chromatographic separation was achieved on a reversed-phase Gemini C₁₈ at 35℃ using acetonitrile,methanol and 0.03% aqueous solution of formic acid(volume ratio 60:15:25) as mobile phase at flow rate of 0.3 mL/min.The UV absorption wavelength was set at 235 nm and the injection volume was 5 μL.Under these conditions,the detection was performed with negative electrospray ionization,monitoring the transitions m/z 359.65→273.70 for bezafibrate and m/z 212.95→127.08 for the clofibric acid(internal standard).A good linearity was achieved within the concentration range of 0.073~7.884 mg/L(r=0.9995) for bezafibrate and the mean recovery of samples ranged from 94.3% to 103.1% at three tested concentrations.The intra-day and inter-day precisions(as relative standard deviation) were below 6%.The limit of quantification(LOQ) was 30 μg/L and the limit of detection(LOD) was 9 μg/L.This HPLC-MS method was reproducible and sensitive enough for routine analysis of bezafibrate in human plasma.
- bezafibrate,
- HPLC-MS,
- rat plasma
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