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Citation: CUI Fengling, HUO Ruina, ZHANG Guisheng, XING Weiwei. Fluorescence Spectrometry on the Interaction of 4'-O-(α-L-oleandrosyl)daunorubicin with Calf Thymus DNA[J]. Chinese Journal of Applied Chemistry, ;2012, 29(2): 238-244. doi: 10.3724/SP.J.1095.2012.00116 shu

Fluorescence Spectrometry on the Interaction of 4'-O-(α-L-oleandrosyl)daunorubicin with Calf Thymus DNA

1.
School of Chemistry and Environmental Science, Henan Normal University, Xinxiang 453007, China

Corresponding author: CUI Fengling,
Received Date: 24 March 2011
Available Online: 2 June 2011
Fund Project: 国家自然科学基金资助项目(30970696) (30970696)

The interaction of 4'-O-(α-L-oleandrosyl)daunorubicin(ODNR) with calf thymus DNA(ctDNA) in Tris-HCl buffer solution(pH=7.4) was studied by fluorescence and ultraviolet absorption spectroscopies.Effects of the ionic strength,KI quenching,and the ODNR binding differences between the single stranded DNA(ssDNA) and the double stranded DNA(dsDNA) were studied.The results showed that the major binding mode between ODNR and ctDNA was intercalation.The intrinsic fluorescence of ODNR was quenched by ctDNA through static quenching procedure.The binding constants(K) and sites(n) at different temperatures were obtained by Scatchard equation.The predominant intermolecular forces are likely the hydrophobic and electrostatic interactions according to the thermodynamic parameters.
- ODNR,
- ctDNA,
- interaction,
- fluorescence spectrum,
- intercalative binding
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