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Citation: NING Aimin, DANG Yuli, ZHAO Shiju, WAN Xinsheng, ZHENG Xianfu. Study on the Interaction of Protein with Quaternaryammonium Gemini Surfactants by Electromotive Force Method[J]. Chinese Journal of Applied Chemistry, ;2011, 28(7): 826-830. doi: 10.3724/SP.J.1095.2011.00534 shu

Study on the Interaction of Protein with Quaternaryammonium Gemini Surfactants by Electromotive Force Method

1.
College of Science, Henan Agricultural University, Zhengzhou 450002

Corresponding author: NING Aimin,
Received Date: 14 September 2010
Available Online: 2 November 2010
Fund Project: 河南省重大科技攻关计划(102101310600)资助项目 (102101310600)

An ion selective electrode of quaternary-ammonium gemini surfactant C₁₂-3-C₁₂·2Br has been fabricated.The variation of electromotive force(EMF) of the systems of C₁₂-3-C₁₂·2Br and bovine serum albumin(BSA) with the concentration of C₁₂-3-C₁₂·2Br has been studied.The binding isotherms and Scatchard plots of the interaction of C₁₂-3-C₁₂·2Br with BSA molecules have been obtained.The numbers of binding sites,Hill coefficient and Hill binding constant have been calculated on the basis of the binding capacity.The results show that the interaction of C₁₂-3-C₁₂·2Br with BSA molecules behaved a positive cooperativity in the binding process.There are two types of binding sites on BSA for C₁₂-3-C₁₂·2Br.One is high-affinity binding site at which the essential role of the interaction is electrostatic force,and the other is low-affinity binding site at which the essential role of interaction is hydrophobic force.
- quaternary-ammonium gemini surfactant,
- electromotive force,
- bovine serum albumin,
- binding isotherm,
- binding capacity
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