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Citation: LV Qianqian, GAO Suya, XIA Donghui, LI Hua. Fluorescence Spectroscopy on the Interaction of Diacerein and Human Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2011, 28(7): 836-841. doi: 10.3724/SP.J.1095.2011.00497 shu

Fluorescence Spectroscopy on the Interaction of Diacerein and Human Serum Albumin

LV Qianqian ^a ,
GAO Suya ^a,b ,
XIA Donghui ^a ,
LI Hua ^a,,

1.
a Institute of Analytical Science, Northwest University, Xi'an 710069;
2.
b Department of Pharmacy, Xi'an Medical University, Xi'an

Corresponding author: LI Hua,
Received Date: 26 August 2010
Available Online: 15 November 2010
Fund Project: 国家自然科学基金(20975081)资助项目 (20975081)

The interaction of diacerein with human serum albumin(HSA) in physiological solution was studied by fluorescence spectroscopy.The result shows that diacerein quenches the fluorescence of HSA in a static quenching mode.The binding equilibrium constant K_A and numbers of binding site n at different temperatures were calculated using two methods,which were compared in the work.The thermodynamic parameters obtained from measured data showed that the binding of diacerein to HSA involved hydrogen bonding and van der Waals interactions.According to the theory of energy transfer,the binding distance(r=2.88 nm) and transfer efficiency of energy(E=0.273 8) between diacerein and HSA were determined.The effect of diacerein on the conformation of HSA was also analyzed by synchronous fluorescence spectrometry.
- diacerein,
- human serum albumin,
- fluorescence spectroscopy,
- interaction
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