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Citation: ZHAO Sucai, ZHANG Mei, WAN Lingli, SONG Yonghai, WANG Li. Preparation and Electrocatalytic Behaviors of GCE/DNA/PFS/RTIL/HRP Modified Electrode[J]. Chinese Journal of Applied Chemistry, ;2011, 28(3): 333-337. doi: 10.3724/SP.J.1095.2011.00291 shu

Preparation and Electrocatalytic Behaviors of GCE/DNA/PFS/RTIL/HRP Modified Electrode

1.
College of Chemistry & Chemical Engineering, Jiangxi Normal University, Nanchang 330022

Corresponding author: WANG Li,
Received Date: 17 May 2010
Available Online: 5 July 2010
Fund Project: 国家自然科学基金(20705011,20905032) (20705011,20905032)江西省自然科学基金(2007GZW0174,2008GZH0028) (2007GZW0174,2008GZH0028)江西省教育厅科技项目(GJJ09455,GJJ10389) (GJJ09455,GJJ10389)江西师范大学科研启动基金和江西师范大学研究生创新基金专项资金(JXSD-Y-09086)资助项目 (JXSD-Y-09086)

A modified electrode was developed by immobilizing horseradish peroxidase (HRP) onto a biocompatible glassy carbon electrode (GCE) modified with a DNA/poly (ferrocenylsilane) (PFS)/room temperature ionic liquid(RTIL) film and the as-prepared GCE/DNA/PFS/RTIL/HRP electrode provides both a biocompatible microenvironment for HRP to keep its good bioactivity and an effective pathway of electron transfer between the redox center of HRP and the electrode surface. The modified electrode showed a good electrocatalytic behavior toward the reduction of H₂O₂. The electrocatalytic behaviors of GCE/DNA/PFS/RTIL/HRP to the reduction of H₂O₂ were investigated by cyclic voltammograms and amperometric technique in detail. Under the optimized conditions, the modified electrode shows fast response to the electrocatalytic oxidation of H₂O₂ and the steady current was observed within 2 s. The catalytic current is linear to the H₂O₂ concentration in the range of 3.25 μmol/L to 1.47 mmol/L and 1.86 mmol/L to 5.35 mmol/L. The detection limit was 0.86 μmol/L based on the criterion of a signal-to-noise ratio of 3. The biosensor showed high sensitivity, good reproducibility and stability. Furthermore, the modified electrode also showed a good electrocatalytic behavior toward the reduction of O₂.
- DNA,
- horseradish peroxidase,
- hydrogen peroxide,
- poly (ferrocenylsilane),
- room temperature ionic liquid
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