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Citation: CAI Yu, DU Tao, ZHAO Shengli, GAO Chunfang. Regeneration of IMAC-Cu Proteinchip by Phosphate Buffer Solution[J]. Chinese Journal of Applied Chemistry, ;2011, 28(2): 219-223. doi: 10.3724/SP.J.1095.2011.00211 shu

Regeneration of IMAC-Cu Proteinchip by Phosphate Buffer Solution

CAI Yu ^a ,
DU Tao ^b ,
ZHAO Shengli ^b,, ,
GAO Chunfang ^c

1.
a Information Management Institute, Henan University of Science & Technology, Luoyang 471003;
2.
b Materials Institute, Henan University of Science & Technology, Luoyang 471003;
3.
c 150 Hospital of Chinese People's Liberation Army, Luoyang

Corresponding author: ZHAO Shengli,
Received Date: 12 April 2010
Available Online: 15 July 2010
Fund Project: 国家自然科学基金(20504026)资助项目 (20504026)河南省基础与前沿研究计划(072300440060) (072300440060)

The effects of pH value and eluent of phosphate buffer solution on the regeneration of immobilized metal affinity capture arrays-Cu(IMAC-Cu) proteinchip was studied by scanning electron microscope(SEM) and SELDI-TOF-MS proteinchip system.The results show that the adsorbed proteins can be easily removed.However,the negative influence on chemical structure of the proteinchip is getting worse with decreasing the pH value of the phosphate buffer solution.Adding eluent of EDTA or Gly in the phosphate buffer solution can obviously improve the protein desorption.The best regeneration results of IMAC-Cu proteinchip by phosphate solution are gained at pH=6.5 with using 0.02 mol/L EDTA as eluent for 72 h.
- proteinchip,
- phosphate buffer solution,
- regeneration
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