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Citation: LIAO Minfu, LIN Cuiwu, HUANG Li, XU Zijing, QIN Sasa, LI Aiyuan. Isolation, Purification and Hemostatic Activity of Water-soluble Polysaccharide in Blumea Riparia(BI.) DC.[J]. Chinese Journal of Applied Chemistry, ;2011, 28(1): 83-87. doi: 10.3724/SP.J.1095.2011.00124 shu

Isolation, Purification and Hemostatic Activity of Water-soluble Polysaccharide in Blumea Riparia(BI.) DC.

LIAO Minfu ^a ,
LIN Cuiwu ^a,, ,
HUANG Li ^a ,
XU Zijing ^a ,
QIN Sasa ^a ,
LI Aiyuan ^b

1.
a School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004;
2.
b GuangXi Traditional Chinese Medical University, Nanning

Corresponding author: LIN Cuiwu,
Received Date: 3 March 2010
Available Online: 21 April 2010
Fund Project: 国家自然科学基金(20662001,20962002)资助项目 (20662001,20962002)2007年国家大学生创新实验项目(071059314) (071059314)

Water-soluble polysaccharide B(BRP-B) in Blumea riparia(BI.) DC. was extracted and purified by using membrane separation, discoloration on diethylaminoethyl(DEAE)-cellulose, and sephadex column chromatography of G10 and G50. Results from high-performance gel permeation chromatography (HPGPC) showed that the obtained BRP-B has a narrow-distributed molecular mass, with the number average molecular mass and mass average molecular mass as 2654 and 2716, respectively. The drug activity test based on plasma recalcification as the index showed that BRP-B with the optimal concentration of 6.25×10^-2 g/L had the strongest procoagulant activity, the coagulation time was 248.52 s, and the inhibition rate of coagulation time was 22.42%. In particularly, BRP-B with concentration of 1.67×10^-2, 1.97×10^-2 and 2.85×10^-2 g/L could significantly enhance the frequency of uterus muscle.
- Blumea riparia(BI.)DC.,
- polysaccharide,
- separation,
- purification,
- hemostatic activity
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