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Citation: SU Xiao-Dong, JIA Yun, LI Yi. Detection of Ascorbic Acid Using the Plasmonic Resonance Light Scattering Method Based on Silver Nanoparticle Probe[J]. Chinese Journal of Applied Chemistry, ;2010, 27(12): 1474-1477. doi: 10.3724/SP.J.1095.2010.00125 shu

Detection of Ascorbic Acid Using the Plasmonic Resonance Light Scattering Method Based on Silver Nanoparticle Probe

SU Xiao-Dong ^a, ,
JIA Yun ^a ,
LI Yi ^b

a. College of Chemistry and Chemical Engineering, Chongqing University of Science and Technology, Chongqing 401331;
b. Southwest Research & Design Institute of Chemical Industry, Chengdu

Received Date: 3 March 2010
Revised Date: 1 June 2010

A resonance light scattering detection method of ascorbic acid is reported based on the formation of silver nanoparticles(NPs).Ascorbic acid(vitamin C,VC) could reduce Ag(NH₃)₂⁺ to form the silver nanoparticles,resulting in strong plasmon resonance light scattering(PRLS) signals characterized at 544 nm,which could be measured using a common spectrofluorometer.It is found that the PRLS intensities were proportional to the ascorbic acid concentrations in the range of 0.2~7.0 μmol/L with R²=0.9988 and detection limit(3σ/k) of 8.1 × 10^-9 mol/L. The presence of a physically adsorbed layer of the product of the redox reaction on the surface of colloidal silver could prevent irreversible aggregation of silver NPs. The conditions of the reaction and the interference of the common ions are investigated. For the determination of ascorbic acid in VC tablets and beverage samples.The recovery was within the range of 94.5%~97.0%. Results suggested that the method possessed easy of implementation,high sensitivity,and better recovery,and so on.
- detection,
- silver nanoparticles,
- resonance light scattering,
- ascorbic acid
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