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Citation: CAI Xue-Mei, LI Jian-Qing, WEI Yan-Li, DONG Chuan. Interactions Between an Intramolecular Charge Transfer Fluorescence Probe 1-Keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene and Human Serum Albumin[J]. Chinese Journal of Applied Chemistry, ;2010, 27(11): 1313-1317. doi: 10.3724/SP.J.1095.2010.00013 shu

Interactions Between an Intramolecular Charge Transfer Fluorescence Probe 1-Keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene and Human Serum Albumin

CAI Xue-Mei ^a,b, ,
LI Jian-Qing ^a,b ,
WEI Yan-Li ^a ,
DONG Chuan ^a

a. Environmental-science and Engineering-study Center, Shanxi University, Taiyuan 030006;
b. College of Chemistry and Chemical Engineering, Jinzhong University, Jinzhong

Received Date: 6 January 2010
Revised Date: 30 April 2010

The interactions between 1-Keto-2-(p-dimethylaminobenzal)-tetrohydronaphthalene(KDTN) and human serum albumin(HSA) have been studied by fluorescence and UV-Vis absorption spectra.The binding of KDTN to HSA quenches the tryptophan residue fluorescence and the results show that static quenching occurrs together with the complex formation.The binding constant and binding sites of KDTN to HSA at pH=7.43 are measured at different temperatures according to the double logarithm regression curve.In addition,the thermodynamic data show that KDTN molecules enter the hydrophobic cavity of HSA via Van der Waals force or hydrogen bond.Finally,the synchronous fluorescence spectra show that the microenvironment of the tryptophan residues does not show significant effect on the conformation.
- Keto-(p-dimethylaminobenzal)-tetrohydronaphthalene,
- human serum albumin,
- fluorescence spectra
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