Citation: YU Changmu, ZHANG Rong, LU Xiaoluan, YANG Min, PENG Qianrong. Preparation of Imm-Fe³⁺-IL with Peroxidase Activity and Its Colorimetric Determination of H₂O₂ and Glucose[J]. Chinese Journal of Applied Chemistry, ;2020, 37(10): 1211-1220. doi: 10.11944/j.issn.1000-0518.2020.10.200064 shu

Preparation of Imm-Fe³⁺-IL with Peroxidase Activity and Its Colorimetric Determination of H₂O₂ and Glucose

YU Changmu ^a ,
ZHANG Rong ^b ,
LU Xiaoluan ^a ,
YANG Min ^a,, ,
PENG Qianrong ^c,,

a.
School of Pharmacy, Guizhou University, Guiyang 550025, China
b.
College of Chemistry and Chemical Engineering, Guizhou University, Guiyang 550025, China
c.
Technology Center of Guizhou China Tobacco Industry Co., Ltd, Guiyang 550009, China

Corresponding author: YANG Min, 2578973180@qq.com PENG Qianrong, 3435391@qq.com
Received Date: 12 March 2020
Revised Date: 19 May 2020
Accepted Date: 11 June 2020

Fund Project: Supported by the National Natural Science Foundation of China(No.21562014), Guizhou Zhongyan Technology Project(Qianyan Gongji [2015] No.8)Guizhou Zhongyan Technology Project Qianyan Gongji [2015] No.8the National Natural Science Foundation of China 21562014

Figures(8)

The Imm-Fe³⁺-IL nanomaterials prepared by the sol-gel method have peroxidase-like activity, which can catalyze the rapid oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB) with hydrogen peroxide (H₂O₂) to produce corresponding color changes. The steady-state kinetic analysis shows that the catalytic kinetics of Imm-Fe³⁺-IL follows the typical Michaelis-Menten model, and the Ping-Pong mechanism. Compared with horseradish peroxidase (HRP), Imm-Fe³⁺-IL nanomaterials have stronger affinity. Combined with glucose oxidase, a colorimetric detection method for H₂O₂ and glucose is established. The results show that the concentration of H₂O₂ and glucose shows a good linear relationship with the absorbance of the reaction system. The linear range of H₂O₂ is 1~200 μmol/L, the linear range of glucose is 10~200 μmol/L, and the limits of detection (LOD) are 0.35 and 3.31 μmol/L for H₂O₂ and glucose, respectively.
- ionic liquid,
- peroxidase-like,
- colorimetric detection,
- H₂O₂,
- glucose
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Preparation of Imm-Fe3+-IL with Peroxidase Activity and Its Colorimetric Determination of H2O2 and Glucose

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通讯作者: 陈斌, bchen63@163.com

Preparation of Imm-Fe³⁺-IL with Peroxidase Activity and Its Colorimetric Determination of H₂O₂ and Glucose