Citation: HUANG Xuewen, XU Sheng, ZHAO Wei, WEI Wei, LI Xiaojie, LIU Xiaoya. Hydrogen Peroxide Sensor Based on a Polymeric Self-assembled Nanoparticles-Modified Screen-Printed Electrode[J]. Chinese Journal of Applied Chemistry, ;2020, 37(2): 235-241. doi: 10.11944/j.issn.1000-0518.2020.02.190190 shu

Hydrogen Peroxide Sensor Based on a Polymeric Self-assembled Nanoparticles-Modified Screen-Printed Electrode

Key Laboratory of Synthetic and Biological Colloids, Ministry of Education, School of Chemical and Material Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China

Corresponding author: LIU Xiaoya, lxy@jiangnan.edu.cn
Received Date: 8 July 2019
Revised Date: 16 August 2019
Accepted Date: 9 September 2019

Fund Project: the National Natural Science Foundation of China 51803079Supported by the National Natural Science Foundation of China(No.51803079, No.51573073)the National Natural Science Foundation of China 51573073

Figures(6)

We report on a facile and efficient construction of electrochemical biosensors by modifying the screen printed carbon electrodes (SPCE) with multifunctional polymeric nanoparticles. An amphiphilic random copolymer, poly(St-co-AA-co-VCz-co-DMAEMA) (PSACD), was first synthesized using styrene (St), acrylic acid (AA), N-vinylcarbazole (VCz) and dimethylaminoethyl methacrylate (DMAEMA) as hydrophobic, hydrophilic, electroactive and enzymatic compatible monomers, respectively. The polymeric nanoparticles (PSACD NPs) were then prepared through self-assembly of polymers in a selective solvent mixture of DMF/H₂O. The obtained PSACD NPs were characterized by particle size analyzer and scanning electron microscope (SEM), and were used to fabricate the hydrogen peroxide (H₂O₂) biosensor with the functions of improving the specific surface areas, providing a suitable microenvironment for keeping the enzyme activity, and accelerating the electron transfer between enzymes and the electrodes. Specifically, the SPCE was successively modified by PSACD NPs suspensions, horseradish peroxidase (HRP) solution and perfluorosulfonic acid-PTFE copolymer (Nafion) solution. The properties of the proposed electrochemical biosensor were studied via an amperometric detection method. The results show that the biosensor has a short response time (less than 2 s) and a linear increasing response current with the concentration of H₂O₂ increasing from 0.02 to 7.48 mmol/L. The biosensor also has nice stability, good selectivity and excellent anti-interference performance.
- polymeric nanoparticles,
- self-assembly,
- screen-printed carbon electrode,
- horseradish peroxidase,
- biosensor
1. [1]
  HUANG Qilin. Study on Novel Enzyme Biosensors Based on Functionalized Nanomaterials and Their Applications[D]. Shanghai: East China Normal University, 2013(in Chinese).
2. [2]
  Chaiyo S, Mehmeti E, Žagar K. Electrochemical Sensors for the Simultaneous Determination of Zinc, Cadmium and Lead Using A Nafion/Ionic Liquid/Graphene Composite Modified Screen-Printed Carbon Electrode[J]. Anal Chim Acta, 2016,918:26-34. doi: 10.1016/j.aca.2016.03.026
3. [3]
  Rungsawang T, Punrat E, Adkins J. Development of Electrochemical Paper-Based Glucose Sensor Using Cellulose-4-Aminophenylboronic Acid-Modified Screen-Printed Carbon Electrode[J]. Electroanalysis, 2016,28(3):462-468. doi: 10.1002/elan.201500406
4. [4]
  Hu X, Goud K Y, Kumar V S. Disposable Electrochemical Aptasensor Based on Carbon Nanotubes-V₂O₅-Chitosan Nanocomposite for Detection of Ciprofloxacin[J]. Sens Actuators B, 2018,268:278-286. doi: 10.1016/j.snb.2018.03.155
5. [5]
  Su W, Wang S, Cheng S. H. Electrochemically Pretreated Screen-Printed Carbon Electrodes for the Simultaneous Determination of Aminophenol Isomers[J]. J Electroanal Chem, 2011,651(2):166-172.
6. [6]
  SUN Jianjun, WEI Hang, LIN Zhibin, et al. Pretreatment Method of Screen-Printed Carbon Electrodes: CN, 101235502[P]. 2008-08-06(in Chinese).
7. [7]
  González-Sánchez M, Gómez-Monedero B, Agrisuelas J. Highly Activated Screen-Printed Carbon Electrodes by Electrochemical Treatment with Hydrogen Peroxide[J]. Electrochem Commun, 2018,91:36-40. doi: 10.1016/j.elecom.2018.05.002
8. [8]
  YANG Shaoming, CHEN Yansheng, LI Ruiqin. One-step Preparation of Horseradish Peroxidase Biosensor via Electrodeposition[J]. Chinese J Appl Chem, 2015,32(7):849-854.
9. [9]
  Li Z, Sheng L, Meng A. A Glassy Carbon Electrode Modified with a Composite Consisting of Reduced Graphene Oxide, Zinc Oxide and Silver Nanoparticles in a Chitosan Matrix for Studying the Direct Electron Transfer of Glucose Oxidase and for Enzymatic Sensing of Glucose[J]. Microchim Acta, 2016,183(5):1625-1632. doi: 10.1007/s00604-016-1791-x
10. [10]
  Lee S W, Lee K Y, Song Y W. Direct Electron Transfer of Enzymes in a Biologically Assembled Conductive Nanomesh Enzyme Platform[J]. Adv Mater, 2016,28(8):1577-1584. doi: 10.1002/adma.201503930
11. [11]
  Kanso H, García M B G, Llano L F. Novel Thin Layer Flow-Cell Screen-Printed Graphene Electrode for Enzymatic Sensors[J]. Biosens Bioelectron, 2017,93:298-304. doi: 10.1016/j.bios.2016.08.069
12. [12]
  Arduini F, Forchielli M, Amine A. Screen-Printed Biosensor Modified with Carbon Black Nanoparticles for the Determination of Paraoxon Based on the Inhibition of Butyrylcholinesterase[J]. Microchim Acta, 2015,182(3/4):643-651.
13. [13]
  Hernández-Ibáñez N, García-Cruz L, Montiel V. Electrochemical Lactate Biosensor Based upon Chitosan/Carbon Nanotubes Modified Screen-Printed Graphite Electrodes for the Determination of Lactate in Embryonic Cell Cultures[J]. Biosens Bioelectron, 2016,77:1168-1174. doi: 10.1016/j.bios.2015.11.005
14. [14]
  KULISONG Hayierbieke, ZENG Han. Direct Electrochemical Behavior and Sensing Performance of Nitrogen Doped Meso Porous Carbon and Chitosan Composite Immobilized with Laccase Modified Electrode[J]. Chinese J Appl Chem, 2013,30(10):1194-1201.
15. [15]
  Arduini F, Micheli L, Moscone D. Electrochemical Biosensors Based on Nanomodified Screen-Printed Electrodes:Recent Applications in Clinical Analysis[J]. TrAC Trends Anal Chem, 2016,79:114-126. doi: 10.1016/j.trac.2016.01.032
16. [16]
  Nieto-Suárez M, López-Quintela M A, Lazzari M. Preparation and Characterization of Crosslinked Chitosan/Gelatin Scaffolds by Ice Segregation Induced Self-assembly[J]. Carbohydr Polym, 2016,141:175-183. doi: 10.1016/j.carbpol.2015.12.064
17. [17]
  Harrison A, Vuong T T, Zeevi M P. Rapid Self-Assembly of Metal/Polymer Nanocomposite Particles as Nanoreactors and Their Kinetic Characterization[J]. Nanomaterials, 2019,9(3)318. doi: 10.3390/nano9030318
18. [18]
  Seo E, Lee T, Lee K T. Versatile Double Hydrophilic Block Copolymer:Dual Role as Synthetic Nanoreactor and Ionic and Electronic Conduction Layer for Ruthenium Oxide Nanoparticle Supercapacitors[J]. J Mater Chem, 2012,22(23):11598-11604. doi: 10.1039/c2jm30738c
19. [19]
  Heidari A, Younesi H, Mehraban Z. Selective Adsorption of Pb(Ⅱ), Cd(Ⅱ), and Ni(Ⅱ) Ions from Aqueous Solution Using Chitosan-MAA Nanoparticles[J]. Int J Biol Macromol, 2013,61:251-263. doi: 10.1016/j.ijbiomac.2013.06.032
20. [20]
  Zhang S, Shao Y, Yin G. Self-assembly of Pt Nanoparticles on Highly Graphitized Carbon Nanotubes as an Excellent Oxygen-Reduction Catalyst[J]. Appl Catal B, 2011,102(3/4):372-377.
21. [21]
  Amalvy J I, Armes S P, Binks B P. Use of Sterically-Stabilised Polystyrene Latex Particles as a pH-Responsive Particulate Emulsifier to Prepare Surfactant-Free Oil-in-Water Emulsions[J]. Chem Commun, 2003(15):1826-1827. doi: 10.1039/b304967a
22. [22]
  Yuan W, Zhang J, Zou H. Synthesis, Crystalline Morphologies, Self-assembly, and Properties of H-Shaped Amphiphilic Dually Responsive Terpolymers[J]. Polym Sci Part A:Polym Chem, 2012,50(13):2541-2552. doi: 10.1002/pola.26032
23. [23]
  Huang S, Jiang S. Structures and Morphologies of Biocompatible and Biodegradable Block Copolymers[J]. RSC Adv, 2014,4(47):24566-24583. doi: 10.1039/C4RA03043E
24. [24]
  YANG Lin. Study on Synthesis and Properties of Star Homo- and Block Copolymers of 4-Arm Dimethylaminoethyl Methacrylate[D]. Xi'an: Shaanxi Normal University, 2007(in Chinese).
25. [25]
  Prasad K S, Muthuraman G, Zen J M. The Role of Oxygen Functionalities and Edge Plane Sites on Screen-Printed Carbon Electrodes for Simultaneous Determination of Dopamine, Uric Acid and Ascorbic Acid[J]. Electrochem Commun, 2008,10(4):559-563. doi: 10.1016/j.elecom.2008.01.033
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