Citation: ZHANG Shouyan, HU Jianglei, SHI Xincui, ZHANG Peibiao, ITO Yoshihiro. Preparation of Electroactive and Bioactive DOPA-Jointed Insulin-Like Growth Factor-1@Poly(glycolide-lactide)/Poly(3-hexylthiophene) Electrospinning Fiber and Its Application in Neural Tissue Engineering[J]. Chinese Journal of Applied Chemistry, ;2019, 36(9): 1003-1014. doi: 10.11944/j.issn.1000-0518.2019.09.190027 shu

Preparation of Electroactive and Bioactive DOPA-Jointed Insulin-Like Growth Factor-1@Poly(glycolide-lactide)/Poly(3-hexylthiophene) Electrospinning Fiber and Its Application in Neural Tissue Engineering

a.
School of Chemical Engineering & Advanced Institute of Materials Science, Changchun University of Technology, Changchun 130012, China
b.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022 China
c.
Nano Medical Engineering Laboratory, RIKEN, Japan

Corresponding author: HU Jianglei, hujl863@163.com SHI Xincui, xcsh@ciac.ac.cn ZHANG Peibiao, zhangpb@ciac.ac.cn
Received Date: 25 January 2019
Revised Date: 26 March 2019
Accepted Date: 24 April 2019

Fund Project: Supported by the Program of Scientific Development of Jilin Province(No.20170520141JH)the Program of Scientific Development of Jilin Province 20170520141JH

Figures(8)

An ideal biomaterial for nerve repair should have the properties of similar electrical properties to normal nerve, biomimetic extracellular matrix structure and the release of specific growth factors. In this study, composite fibers with electroactive and bionic structures were prepared by electrospinning process by incorporating various mass fractions(0, 3%, 5%, 10%) poly(3-hexylthiophene)(P3HT) into the poly(glycolide-lactide)(PLGA) polymer. DOPA-jointed insulin-like growth factor-1(DOPA-IGF-1) with different concentrations(10, 50, 100 ng/mL) was bound to the fiber surface using tyrosine hydroxylase to achieve long-term and stable release of growth factor. The fiber diameter and surface hydrophilic properties of the material were characterized by scanning electron microscopy and contact angle measurement, respectively. The biocompatibility and bioactivity of the fibers in vitro were evaluated by cell culture and fluorescence staining experiments. It was found that the electroactive fiber could effectively promote the proliferation of rat adrenal pheochromocytoma cells(PC12 cells), and PLGA/P3HT-5% fiber showed better cell responsiveness. Fibers incorporated with DOPA-IGF-1 with a concentration of 10 ng/mL were more conducive to PC12 cell adhesion and growth. The results showed that DOPA-IGF-1@PLGA/P3HT with both electrical and biological activity had potential application in neural tissue repair.
- electroactive,
- growth factor,
- polythiophene,
- polyester composite,
- electrospinning,
- neural tissue engineering
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