Citation: SHI Xin, LIU Chuanzhi, GONG Ping, LI wei, HOU Yue. Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys[J]. Chinese Journal of Applied Chemistry, ;2019, 36(7): 847-854. doi: 10.11944/j.issn.1000-0518.2019.07.190047 shu

Hydrogen Peroxide and Glucose Sensitive Colorimetric Method Based on Deuterohemin-Ala His Thr Val Glu Lys

School of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China

Corresponding author: HOU Yue, hy46155@126.com
Received Date: 21 February 2019
Revised Date: 26 March 2019
Accepted Date: 5 May 2019

Fund Project: the Scientific and Technological Research Project Foundation of Jilin Provincial Science and Technology Department 20170204029SFSupported by the Scientific and Technological Research Project Foundation of Jilin Provincial Science and Technology Department(No.20170204029SF)

Figures(8)

A simple colorimetric method for determination of H₂O₂ and glucose was established based on the peroxidase-like activity of deuterohemin-Ala His Thr Val Glu Lys(DhHP-6) to catalyze the oxidation of 4-aminoantipyrine(4-APP)-chlorophenol color reaction system in the presence of H₂O₂. The effects of pH, substrate concentration and DhHP-6 concentration were observed, and the reaction linearity, stability, correlation and recovery rate of the colorimetric method were detected. Under optimal reaction conditions, the activity of DhHP-6 is superior to that of peroxidase(POD) under different temperatures and times; the Michaelis constant(K_m) and maximum reaction rate(v_max) of DhHP-6 catalyzing H₂O₂ are 0.171 mmol/L and 4.22×10^-6 mol/s, respectively; the linearity range of H₂O₂ response is 0.39~25.0 mmol/L, the coefficient of variation(CV) and spiked recovery of the high, medium and low levels are between 1.29%~2.16% and 94.5%~101.1%, respectively; and correlation coefficient with the commercial kit is 0.9946; the concentration of glucose in 36 blood samples are 4.26~17.48 mmol/L. There is no significant difference between the two sets of data for glucose assay kit(P>0.05). Therefore, this is a simple, cheap, convenient and sensitive colorimetric determination method.
- hydrogen peroxide,
- deuterohemin-Ala His Thr Val Glu Lys,
- mimetic enzyme,
- colorimetric analysis
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