Citation: LI Yingjie, LIN Xiaotong, GAO Lidi, QIN Shili, TANG Yimin, JIN Zhixiang, ZHANG Shuai. Separation and Analysis of Mixed Amino Acid Enantiomers by Capillary Electrochromatography-Electrospray-Time of Flight/Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, ;2019, 36(7): 823-831. doi: 10.11944/j.issn.1000-0518.2019.07.190021 shu

Separation and Analysis of Mixed Amino Acid Enantiomers by Capillary Electrochromatography-Electrospray-Time of Flight/Mass Spectrometry

College of Chemistry and Chemical Engineering, Qiqihar University, Qiqihar, Heilongjiang 161006, China

Corresponding author: GAO Lidi, gaolidi@163.com
Received Date: 22 January 2019
Revised Date: 27 March 2019
Accepted Date: 26 April 2019

Fund Project: Supported by the Heilongjiang Provincial Department of Education Overseas Scholars Research Funding Project(No.1254HQ012), Heilongjiang Provincial Department of Education Basic Business Special Science and Engineering Project(No.135209215)Heilongjiang Provincial Department of Education Basic Business Special Science and Engineering Project 135209215the Heilongjiang Provincial Department of Education Overseas Scholars Research Funding Project 1254HQ012

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A simple and efficient capillary electrochromatography-electrospray- time of flight/mass spectrometry(CEC-ESI-TOF/MS) method for the separation and analysis of mixed amino acid enantiomers was established. D, L-arginine, D, L-valine, and D, L-glutamate acid were taken as the research objects. By optimizing the CEC separation conditions and MS detection conditions, the six components of the three mixed amino acid enantiomers were separated within 15 min, and the resolutions were 3.03, 1.59 and 1.37, respectively. It provides a reference method for the separation and analysis of mixed enantiomers.
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