Citation: ZHANG Yajing, JI Peng, HAN Deming, ZHAO Lihui, XU Yajuan, LIU Wenshu. Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells[J]. Chinese Journal of Applied Chemistry, ;2019, 36(5): 564-570. doi: 10.11944/j.issn.1000-0518.2019.05.180351 shu

Preparation of Graphene Nano Drug-Loading System and Its Killing Effect on Human Oral Squamous Cell Carcinoma Cells

ZHANG Yajing ^a ,
JI Peng ^a ,
HAN Deming ^a ,
ZHAO Lihui ^a,, ,
XU Yajuan ^b,, ,
LIU Wenshu ^c

a.
College of Life Science and Technology, Changchun University of Science and Technology, Changchun 130022, China
b.
Jilin Cancer Hospital, Changchun 130012, China
c.
The First Hospital of Jilin University, Changchun 130021, China

Corresponding author: ZHAO Lihui, 1252042806@qq.com XU Yajuan, 535923996@qq.com
Received Date: 6 November 2018
Revised Date: 14 January 2019
Accepted Date: 28 February 2019

Fund Project: the Jilin Provincial Science and Technology Agen JJKH20170604KJSupported by the Jilin Provincial Science and Technology Agen(No.JJKH20170604KJ)

Figures(4)

Graphene oxide was synthesized by chemical redox method. The graphene oxide nanoparticles were prepared by ultrasonic fragmentation and peeling, and functionalized by polyethylene glycol(PEG). The modified graphene oxide was loaded with cisplatin. The graphene nano-drug delivery system was characterized by scanning electron microscopy(SEM), ultraviolet-visible absorption spectroscopy(UV-Vis) and Fourier transform infrared spectroscopy(FTIR). The killing effect of graphene nano-drug delivery system on oral squamous cell carcinoma human oral squamous cell carcinoma(KB) cells was examined by cell survival rate experiment(MTT) method. The results demonstrate that the loading rate of graphene nano-drug-loading system is 42.4%. Polyethylene glycol modified nano graphene oxide can reduce the cytotoxicity of nano graphene oxide and improve its biocompatibility. It has dual killing effect on KB cells, which provides a theoretical basis for the clinical application of nano graphene oxide in cancer treatment.
- nano graphene oxide,
- cisplatin,
- cell survival rate experiment,
- human oral squamous cell carcinoma cells,
- cytotoxicity
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