Citation: ZHANG Chenglu, WANG Jing, LI Jingyi, YU Xiangkun, YANG Jingyi, CAI Jinhua, LI Yizheng, WANG Huayu, GONG Rongqing. Synthesis of 1, 2, 4-Triazine-Phenanthroline Co(Ⅲ) Complexes and Their Fluorescence Recognition on Calf Thymus DNA[J]. Chinese Journal of Applied Chemistry, ;2019, 36(2): 212-222. doi: 10.11944/j.issn.1000-0518.2019.02.180161 shu

Synthesis of 1, 2, 4-Triazine-Phenanthroline Co(Ⅲ) Complexes and Their Fluorescence Recognition on Calf Thymus DNA

College of Chemistry and Chemical Engineering, Liaoning Normal University, Dalian, Liaoning 116029, China

Corresponding author: ZHANG Chenglu, zhangchenglu@lnnu.edu.cn
Received Date: 8 May 2018
Revised Date: 27 September 2018
Accepted Date: 15 October 2018

Fund Project: Supported by Technology Research Program of Liaoning Provincial Department of Education(No.2009A426)Technology Research Program of Liaoning Provincial Department of Education 2009A426

Figures(8)

Four 1, 2, 4-triazine-phenanthroling derivatives(ARTP1~ARTP4) were designed and synthesized successfully. ARTP1~ARTP4 were selected to coordinate with Co³⁺ to afford four novel complexes(Co-ARTP1~Co-ARTP4). The structures and properties of ARTP1~ARTP4 and Co-ARTP1~Co-ARTP4 were studied by means of Fourier transform infrared spectrometer(FT-IR), nuclear magnetic resonance(NMR), high resolution mass spectrometry(HRMS) and ultraviolet-visible spectroscopy(UV-Vis). As a result, the excitation peaks of the complexes are weakened and show red shift. The complexes interact with calf thymus DNA(CT-DNA) through the insert mode and the binding constants are K_b=4.78×10⁵, 6.52×10⁵, 5.97×10⁵ and 6.01×10⁵ L/mol, respectively, which indicates that the complexes are expected to be the DNA fluorescence probes. It provides the important theory references for further study on the interaction between small organic molecules with DNA.
- phenanthroline,
- 1, 2, 4-triazine,
- calf thymus DNA,
- fluorescence probe,
- Co-complexes
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