Citation: CHEN Fan, GUO Zhiqian, ZHU Weihong. Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe[J]. Chinese Journal of Applied Chemistry, ;2018, 35(4): 401-409. doi: 10.11944/j.issn.1000-0518.2018.04.170463 shu

Synthesis and Characterization of pH and Temperature Sensitive Polymeric Fluorescent Probe

Institute of Fine Chemicals, East China University of Science & Technology, Shanghai 200237, China

Corresponding author: GUO Zhiqian, guozq@ecust.edu.cn
Received Date: 21 December 2017
Revised Date: 24 January 2018
Accepted Date: 24 January 2018

Fund Project: the National Natural Science Foundation of China 21325625Supported by the National Natural Science Foundation of China(No.21622602, No.21325625)the National Natural Science Foundation of China 21622602

Figures(7)

A pH and thermo-responsive fluorescent polymeric sensor Poly(POSS-NI-NIPAM) was synthesized from naphthalimide(NI), N-isopropyl acrylamide(NIPAM) and polyhedral oligomeric silsesquioxane(POSS) as raw materials by the reversible addition-fragmentation chain transfer polymerization(RAFT). The pH and thermo-responsive properties of Poly(POSS-NI-NIPAM) were characterized by nuclear magnetic resonance spectroscopy(NMR), fluorescence emission spectrometer and mass spectrometry(MS). The results show that Poly(POSS-NI-NIPAM) shows obvious fluorescence changes in the range of pH 5.8~8.0 with a reversible performance. The high sensitivity for pH/temperature makes Poly(POSS-NI-NIPAM) as a promising fluorescent probe for the detection of intracellular pH/temperature in living cells.
- polymeric fluorescent probe,
- pH sensitivity,
- temperature sensitivity,
- polyhedral oligomeric silsesquioxane
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