Citation: WANG Chengke, WANG Zhenxin. Screening of Cu(Ⅱ) Ion Induced β-Amyloid Peptide Aggregation Inhibitor and Their Molecular Structure Analysis[J]. Chinese Journal of Applied Chemistry, ;2016, 33(7): 834-840. doi: 10.11944/j.issn.1000-0518.2016.07.150375 shu

Screening of Cu(Ⅱ) Ion Induced β-Amyloid Peptide Aggregation Inhibitor and Their Molecular Structure Analysis

WANG Chengke ^a,b ,
WANG Zhenxin ^b,,

a.
a. School of Food and Biological Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China;
b.
b. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Corresponding author: WANG Zhenxin,
Received Date: 22 October 2015
Available Online: 11 December 2015
Fund Project:

It is very important to develop efficient drugs for the cure of Alzheimer's disease. Here, gold nanoparticle probes were utilized to acquire the ability of 13 kinds of compounds to inhibit the Cu²⁺ induced β-amyloid peptide aggregation. Ten of then were screened as the effective inhibitors. The relationships between the chemical structures and their inhibitory efficiencies were also studied as well. We also used the screened molecules to regulate the morphology changes of Cu²⁺-β-amyloid aggregates and to decrease the production of H₂O₂ formed by the interaction of Cu²⁺ and β-amyloid peptide. This research is significant to the study of Alzheimer's disease.
- Alzheimer's disease,
- gold nanoparticles,
- inhibitor
1. [1]
  [1] Bu G. Apolipoprotein E and Its Receptors in Alzheimer's Disease:Pathways, Pathogenesis and Therapy[J]. Nat Rev Neurosci,2009,10(5):333-344.
2. [2]
  [2] Bertram L,Tanzi R. Thirty Years of Alzheimer's Disease Genetics:The Implications of Systematic Meta-analyses[J]. Nat Rev Neurosci,2008,9(10):768-778.
3. [3]
  [3] LaFerla F,Green K,Oddo S. Intracellular Amyloid-β in Alzheimer's Disease[J]. Nat Rev Neurosci,2007,8(7):499-509.
4. [4]
  [4] Faller P,Hureau C,La Penna G. Metal Ions and Intrinsically Disordered Proteins and Peptides:From Cu/Zn Amyloid-β to General Principles[J]. Acc Chem Res,2014,47(8):2252-2259.
5. [5]
  [5] Rauk A. The Chemistry of Alzheimer's Disease[J]. Chem Soc Rev,2009,382(9):2698-2715.
6. [6]
  [6] Faller P,Hureau C,Berthoumieu O. Role of Metal Ions in the Self-assembly of the Alzheimer's Amyloid-Β Peptide[J]. Inorg Chem,2013,52(21):12193-12206.
7. [7]
  [7] Porter M R,Kochi A,Karty J A,et al. Chelation-induced Diradical Formation as an Approach to Modulation of the Amyloid-β Aggregation Pathway[J]. Chem Sci,2015,6(2):1018-1026.
8. [8]
  [8] Pithadia A S,Lim M H. Metal-associated Amyloid-β Species in Alzheimer's Disease[J]. Curr Opin Chem Biol,2012,16(1):67-73.
9. [9]
  [9] Lin C J,Huang H C,Jiang Z F. Cu (Ⅱ) Interaction with Amyloid-β Peptide:A Review of Neuroactive Mechanisms in AD Brains[J]. Brain Res Bull,2010,82(5):235-242.
10. [10]
  [10] Folk D S,Franz K J. A Prochelator Activated by β-Secretase Inhibits Aβ Aggregation and Suppresses Copper-Induced Reactive Oxygen Species Formation[J]. J Am Chem Soc,2010,132(14):4994-4995.
11. [11]
  [11] Bonda D J,Lee H,Blair J A,et al. Role of Metal Dyshomeostasis in Alzheimer's Disease[J]. Metallomics,2011,3(3):267-270.
12. [12]
  [12] Huang W,Wei W,Shen Z. Drug-like Chelating Agents:A Potential Lead for Alzheimer's Disease[J]. RSC Adv,2014,4(94):52088-52099.
13. [13]
  [13] Hindo S S,Mancino A M,Braymer J J,et al. Small Molecule Modulators of Copper-induced Aβ Aggregation[J]. J Am Chem Soc,2009,131(46):16663-16665.
14. [14]
  [14] Choi J S,Braymer J J,Park S K,et al. Synthesis and Characterization of IMPY Derivatives That Regulate Metal-Induced Amyloid-β Aggregation[J]. Metallomics,2011,3(3):284-291.
15. [15]
  [15] Cook N P,Torres V,Jain D,et al. Sensing Amyloid-Β Aggregation Using Luminescent Dipyridophenazine Ruthenium (Ⅱ) Complexes[J]. J Am Chem Soc,2011,133(29):11121-11123.
16. [16]
  [16] Hudson S A,Ecroyd H,Kee T W,et al. The Thioflavin T Fluorescence Assay for Amyloid Fibril Detection Can Be Biased by the Presence of Exogenous Compounds[J]. FEBS J,2009,276(20):5960-5972.
17. [17]
  [17] Xia N,Liu L,Harrington M G,et al. Regenerable and Simultaneous Surface Plasmon Resonance Detection of Aβ(1-40) and Aβ(1-42) Peptides in Cerebrospinal Fluids with Signal Amplification by Streptavidin Conjugated to an N-Terminus-Specific Antibody[J]. Anal Chem,2010,82(24):10151-10157.
18. [18]
  [18] Wang C,Wang J,Liu D,et al. Studying Copper (Ⅱ) Ion Induced Interactions of β-Amyloid Peptides Within Living Cells by Gold Nanoparticle Probes[J]. Anal Methods,2010,2(10):1467-1471.
19. [19]
  [19] Wang C,Liu D,Wang Z,et al. Gold Nanoparticle Based Dot-Blot Immunoassay for Sensitively Detecting Alzheimer's Disease Related beta-Amyloid Peptide[J]. Chem Commun,2012,48(67):8392-8394.
20. [20]
  [20] Kim J S,Ahn H S,Cho S M,et al. Detection and Quantification of Plasma Amyloid-β by Selected Reaction Monitoring Mass Spectrometry[J]. Anal Chim Acta,2014,840(35):1-9.
21. [21]
  [21] Jameson L P,Smith N W,Dzyuba S V. Dye-binding Assays for Evaluation of the Effects of Small Molecule Inhibitors on Amyloid(Aβ) Self-assembly[J]. ACS Chem Neurosci,2012,3(11):807-819.
22. [22]
  [22] Saha K,Agasti S,Kim C,et al. Gold Nanoparticles in Chemical and Biological Sensing[J]. Chem Rev,2012,112(5):2739-2779.
23. [23]
  [23] Jans H,Huo Q. Gold Nanoparticle-Enabled Biological and Chemical Detection and Analysis[J]. Chem Soc Rev,2012,41(7):2849-2866.
24. [24]
  [24] Wang C,Wang K,Wang Z. Development of Gold Nanoparticle Based Colorimetric Method for Quantitatively Studying the Inhibitors of Cu²⁺/Zn²⁺ Induced β-Amyloid Peptide Assembly[J]. Anal Chim Acta,2015,858(6):42-48.
25. [25]
  [25] Wang C,Wang Z. Studying the Relationship Between Cell Cycle and Alzheimer's Disease by Gold Nanoparticle Probes[J]. Anal Biochem,2015,489(22):32-37
26. [26]
  [26] Medinas D B,Toledo J C Jr,Cerchiaro G,et al. Peroxymonocarbonate and Carbonate Radical Displace the Hydroxyl-Like Oxidant in the SOD1 Peroxidase Activity Under Physiological Conditions[J]. Chem Res Toxicol,2009,22(4):639-648.
27. [27]
  [27] Liochev S I,Fridovich I. Copper, Zinc Superoxide Dismutase and H₂O₂ Effects of Bicarbonate on Inactivation and Oxidations of NADPH and Urate, and on Consumption of H₂O₂[J]. J Biol Chem,2002,277(38):34674-34678.
28. [28]
  [28] Wang C,Wang J,Liu D,et al. Gold Nanoparticle-based Colorimetric Sensor for Studying the Interactions of β-Amyloid Peptide with Metallic Ions[J]. Talanta,2010,80(5):1626-1631.
29. [29]
  [29] Wang C,Liu D,Wang Z. Resonance Light Scattering as a powerful Tool for sensitive Detection of β-Amyloid Peptide by Gold Nanoparticle Probes[J]. Chem Commun,2011,47(33):9339-9341.
30. [30]
  [30] Inbar P,Bautista M R,Takayama S A,et al. Assay to Screen for Molecules That Associate with Alzheimer's Related β-Amyloid Fibrils[J]. Anal Chem,2008,80(9):3502-3506.
1. [1]
  Bin SUN , Heyan JIANG . Glucose-modified bis-Schiff bases: Synthesis and bio-activities in Alzheimer′s disease therapy. Chinese Journal of Inorganic Chemistry, 2025, 41(7): 1338-1350. doi: 10.11862/CJIC.20240428
2. [2]
  Yihui Song , Shangshang Qin , Kai Wu , Chengyun Jin , Bin Yu . 生物化学在高水平创新型药学人才培养中的交叉融合应用——以去甲基化酶LSD1抑制剂的活性评价为例. University Chemistry, 2025, 40(6): 341-352. doi: 10.12461/PKU.DXHX202406018
3. [3]
  Hong LI , Xiaoying DING , Cihang LIU , Jinghan ZHANG , Yanying RAO . Detection of iron and copper ions based on gold nanorod etching colorimetry. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 953-962. doi: 10.11862/CJIC.20230370
4. [4]
  Ruiqin Feng , Ye Fan , Yun Fang , Yongmei Xia . Strategy for Regulating Surface Protrusion of Gold Nanoflowers and Their Surface-Enhanced Raman Scattering. Acta Physico-Chimica Sinica, 2024, 40(4): 2304020-0. doi: 10.3866/PKU.WHXB202304020
5. [5]
  Hongpeng He , Mengmeng Zhang , Mengjiao Hao , Wei Du , Haibing Xia . Synthesis of Different Aspect-Ratios of Fixed Width Gold Nanorods. Acta Physico-Chimica Sinica, 2024, 40(5): 2304043-0. doi: 10.3866/PKU.WHXB202304043
6. [6]
  Yu Dai , Xueting Sun , Haoyu Wu , Naizhu Li , Guoe Cheng , Xiaojin Zhang , Fan Xia . Determination of the Michaelis Constant for Gold Nanozyme-Catalyzed Decomposition of Hydrogen Peroxide. University Chemistry, 2025, 40(5): 351-356. doi: 10.12461/PKU.DXHX202407052
7. [7]
  Ruifeng CHEN , Chao XU , Jianting JIANG , Tianshe YANG . Gold nanorod/zinc oxide/mesoporous silica nanoplatform: A triple-modal platform for synergistic anticancer therapy. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2272-2282. doi: 10.11862/CJIC.20250117
8. [8]
  Lina Liu , Xiaolan Wei , Jianqiang Hu . Exploration of Subject-Oriented Undergraduate Comprehensive Chemistry Experimental Teaching Based on the “STS Concept”: Taking the Experiment of Gold Nanoparticles as an Example. University Chemistry, 2024, 39(10): 337-343. doi: 10.12461/PKU.DXHX202405112
9. [9]
  Dingwen CHEN , Siheng YANG , Haiyan FU , Hua CHEN , Xueli ZHENG , Weichao XUE , Jiaqi XU , Ruixiang LI . NiOOH-mediated synthesis of gold nanoaggregates for electrocatalytic performance for selective oxidation of glycerol to glycolate. Chinese Journal of Inorganic Chemistry, 2025, 41(11): 2317-2326. doi: 10.11862/CJIC.20250053
10. [10]
  Zian Fang , Qianqian Wen , Yidi Wang , Hongxia Ouyang , Qi Wang , Qiuping Li . The Test Paper for Metal Ion: A Popular Science Experiment Based on Color Aesthetics. University Chemistry, 2024, 39(5): 108-115. doi: 10.3866/PKU.DXHX202310032
11. [11]
  Hong RAO , Yang HU , Yicong MA , Chunxin LÜ , Wei ZHONG , Lihua DU . Synthesis and in vitro anticancer activity of phenanthroline-functionalized nitrogen heterocyclic carbene homo- and heterobimetallic silver/gold complexes. Chinese Journal of Inorganic Chemistry, 2024, 40(12): 2429-2437. doi: 10.11862/CJIC.20240275
12. [12]
  Xiaofang Li , Zhigang Wang . 调节金助催化剂的d_z²占据轨道增强光催化合成H₂O₂. Acta Physico-Chimica Sinica, 2025, 41(7): 100080-0. doi: 10.1016/j.actphy.2025.100080
13. [13]
  Bo YANG , Gongxuan LÜ , Jiantai MA . Corrosion inhibition of nickel-cobalt-phosphide in water by coating TiO₂ layer. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 365-384. doi: 10.11862/CJIC.20240063
14. [14]
  Shunü Peng , Huamin Li , Zhaobin Chen , Yiru Wang . Simultaneous Application of Multiple Quantitative Analysis Methods in Gas Chromatography for the Determination of Active Ingredients in Traditional Chinese Medicine Preparations. University Chemistry, 2025, 40(10): 243-249. doi: 10.12461/PKU.DXHX202412043
15. [15]
  Xiaotian ZHU , Fangding HUANG , Wenchang ZHU , Jianqing ZHAO . Layered oxide cathode for sodium-ion batteries: Surface and interface modification and suppressed gas generation effect. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 254-266. doi: 10.11862/CJIC.20240260
16. [16]
  Zilin Hu , Yaoshen Niu , Xiaohui Rong , Yongsheng Hu . Suppression of Voltage Decay through Ni³⁺ Barrier in Anionic-Redox Active Cathode for Na-Ion Batteries. Acta Physico-Chimica Sinica, 2024, 40(6): 2306005-0. doi: 10.3866/PKU.WHXB202306005
17. [17]
  Yuhang Zhang , Yi Li , Yuehan Cao , Yingjie Shuai , Yu Zhou , Ying Zhou . Regulating the formation type by Ir of intermediates to suppress product overoxidation in photocatalytic methane conversion. Acta Physico-Chimica Sinica, 2026, 42(2): 100173-0. doi: 10.1016/j.actphy.2025.100173
18. [18]
  Yanyan Zhao , Zhen Wu , Yong Zhang , Bicheng Zhu , Jianjun Zhang . Enhancing photocatalytic H₂O₂ production via dual optimization of charge separation and O₂ adsorption in Au-decorated S-vacancy-rich CdIn₂S₄. Acta Physico-Chimica Sinica, 2025, 41(11): 100142-0. doi: 10.1016/j.actphy.2025.100142
19. [19]
  Xinyu Liu , Weiran Hu , Zhengkai Li , Wei Ji , Xiao Ni . Algin Lab: Surging Luminescent Sea. University Chemistry, 2024, 39(5): 396-404. doi: 10.3866/PKU.DXHX202312021
20. [20]
  Gaofeng Zeng , Shuyu Liu , Manle Jiang , Yu Wang , Ping Xu , Lei Wang . Micro/Nanorobots for Pollution Detection and Toxic Removal. University Chemistry, 2024, 39(9): 229-234. doi: 10.12461/PKU.DXHX202311055

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(520)
HTML views(41)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142