Citation: LIN Chunhua, RUI Peixin, LEI Zhiwei, YAN Nan, XU Zhaohui, LIAO Weilin. Rapid Separation and Determination of Four Fatty Acid Monoglycerides by Ultra Performance Convergence Chromatography-Mass Spectrometry[J]. Chinese Journal of Applied Chemistry, ;2016, 33(2): 238-244. doi: 10.11944/j.issn.1000-0518.2016.02.150188 shu

Rapid Separation and Determination of Four Fatty Acid Monoglycerides by Ultra Performance Convergence Chromatography-Mass Spectrometry

LIN Chunhua ^a ,
RUI Peixin ^a ,
LEI Zhiwei ^a ,
YAN Nan ^a ,
XU Zhaohui ^b ,
LIAO Weilin ^a,,

1.
a National Monosaccharide Chemical Synthesis Engineering Research Center;
2.
b Chemistry and Chemical Engineering Department, Jiangxi Normal University, Nanchang 330027, China

Corresponding author: LIAO Weilin,
Received Date: 5 June 2015
Available Online: 29 October 2015
Fund Project:

A method for rapid separation and determination of four fatty acid monoglycerides, via ultra-performance convergence chromatography-mass spectrometry, was developed. Fatty acid monoglycerides were prepared by lipase-catalyzed glycerolysis using sunflower oil as the starting material. The main monoglycerides are monopalmitin, monostearin, monoolein, and monolinolein. The samples were dissolved by n-hexane/isopropanol(7:3 volume ratio), separated in 6 min on the column of ACQUITY UPC² BEH 2-EP(100 mm×2.1 mm, 1.7 μm) with gradient elution of carbon dioxide and methanol/acetonitrile(1:1 volume ratio) system, and then detected by MS detector in the ESI⁺ mode. The results verified that the standard curves for the four components were obtained with good correlation coefficients(R²)>0.9983 in their concentration ranges. The limits of detection(LOD, S/N=3) for the four monoglycerides are in the range of 0.036~0.093 mg/L. The recoveries for the four monoglycerides at three spiked levels(low, middle, high) are 88.50%~110.00% with the relative standard deviations of 1.01%~4.04%. The proposed UPC²-MS method is featured with low detection limit, less time consuming, good separation and low cost. It would provide a new chromatographic technology platform for the detection of fatty acid esters.
- ultra-performance convergence chromatography-mass spectrometry(UPC²-MS),
- fatty acid monoglyceride,
- glycerolysis,
- sunflower oil
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