Citation: Jianfang QIN, Yuying ZHANG, Lijuan JIA, Jiaqi LIANG, Yuxing YANG, Haiying YANG, Xu LIU. Accurate determination of profenofos by Au_25-xAg_x(PET)₁₈ (PET=2-phenylethanethiol) nanocluster-based electrochemical sensors[J]. Chinese Journal of Inorganic Chemistry, ;2026, 42(6): 1164-1174. doi: 10.11862/CJIC.20250378 shu

Accurate determination of profenofos by Au_25-xAg_x(PET)₁₈ (PET=2-phenylethanethiol) nanocluster-based electrochemical sensors

Jianfang QIN ^{1, #} ,
Yuying ZHANG ^{1, #} ,
Lijuan JIA ¹ ,
Jiaqi LIANG ¹ ,
Yuxing YANG ¹ ,
Haiying YANG ^1,, ,
Xu LIU ^{2, 3,,}

1.
Department of Applied Chemistry, Yuncheng University, Yuncheng, Shanxi 044011, China
2.
School of Chemistry, Nanjing University, Nanjing 210023, China
3.
Institute for Energy Research, Jiangsu University, Zhenjiang, Jiangsu 212013, China

Corresponding author: Haiying YANG, hyyang@ycu.edu.cn Xu LIU, xuliu@nju.edu.cn
Received Date: 16 December 2025
Revised Date: 8 April 2026

Figures(7)

Aiming to meet the demand for rapid and sensitive detection of organophosphate residues, a high- performance electrochemical sensor, NF/KbE-CS/Au_25-xAg_x@G/GCE (where NF, KbE, CS, Au_25-xAg_x, G, and GCE represented Nafion, white kidney bean enzyme, chitosan, Au_25-xAg_x(PET)₁₈ (PET=2-phenylethanethiol), multilayer graphene, and glassy carbon electrode, respectively), was rationally fabricated based on the enzyme inhibition principle. In the as-constructed sensing interface, Au_25-xAg_x@G composite acts as an ideal supporting matrix for enzyme immobilization, meanwhile, its outstanding electrical conductivity and distinct bimetallic synergistic effect significantly accelerate interfacial electron transfer efficiency, thus giving rise to obvious amplification of electrochemical detection signals. Furthermore, the synergistic enhancement between Au_25-xAg_x@G and the white KbE-CS composite enables the sensor to realize highly sensitive determination of profenofos.Under optimal experimental conditions, the developed NF/KbE-CS/Au_25-xAg_x@G/GCE sensor presented a good linear relationship (R²=0.988 4) between the inhibition rate of KbE activity and the logarithm of profenofos mass concentration ranging from 10 to 2 200 μg·L^-1, with a detection limit as low as 0.064 μg·L^-1. In addition, the as-fabricated sensor demonstrated satisfactory reproducibility, favorable stability, and strong anti-interference capability, has been successfully applied to the quantitative detection of organophosphate pesticide residues in real samples.
- composite,
- electrochemical sensor,
- profenofos,
- enzyme inhibition
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通讯作者: 陈斌, bchen63@163.com

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Accurate determination of profenofos by Au25-xAgx(PET)18 (PET=2-phenylethanethiol) nanocluster-based electrochemical sensors

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通讯作者: 陈斌, bchen63@163.com

Accurate determination of profenofos by Au_25-xAg_x(PET)₁₈ (PET=2-phenylethanethiol) nanocluster-based electrochemical sensors