Citation: Pengli GUAN, Renhu BAI, Xiuling SUN, Bin LIU. Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(9): 1817-1826. doi: 10.11862/CJIC.20250058 shu

Trianiline-derived aggregation-induced emission luminogen probe for lipase detection and cell imaging

Pengli GUAN ¹ ,
Renhu BAI ¹ ,
Xiuling SUN ¹ ,
Bin LIU ^2,,

1.
College of New Energy and Materials Engineering, Shanxi University of Electronic Science and Technology, Linfen, Shanxi 041000, China
2.
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China

Corresponding author: Bin LIU, liubin@sxu.edu.cn
Received Date: 21 February 2025
Revised Date: 19 May 2025

Figures(12)

As a potential biomarker for breast cancer, the highly sensitive detection of lipase is conducive to improving the accuracy of disease diagnosis. Lipase has the property that it can react with the reactant at the contact surface, such as when a solid in solution reacts with lipase. Illuminated by the lipase catalytic property, a lipase-responsive fluorescent probe BTPA with the aggregation-induced emission (AIE) was designed and synthesized. The probe could be hydrolyzed by the lipase enzyme and emit yellow fluorescence, because it self-aggregated into granulums with the external solution, and the granulums provided a surface to activate the activity of the lipase enzyme. The fluorescence emission intensity of BTPA was linearly correlated with the activity of lipase in a range of 5.0×10^-5-4.5×10^-4 U·mL^-1, and the detection limit was 4.94×10^-6 U·mL^-1. Besides, the probe could be applied in a complex biochemical system investigated by a selectivity and interference experiment. The laser-confocal imaging experiment showed that the fluorescent probe could accurately identify breast cancer cells in situ and emit strong fluorescence.
- lipase,
- fluorescent probe,
- cell imaging,
- cancer cell
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