Citation: Jie WEI, Qing ZHOU, Dandan DING, Xiang JING, Fei LI. Photothermal toxicity of Prussian blue nanoparticles to cervical cancer cells[J]. Chinese Journal of Inorganic Chemistry, ;2025, 41(11): 2345-2357. doi: 10.11862/CJIC.20240435 shu

Photothermal toxicity of Prussian blue nanoparticles to cervical cancer cells

Jie WEI ¹ ,
Qing ZHOU ¹ ,
Dandan DING ¹ ,
Xiang JING ¹ ,
Fei LI ^2,,

1.
Henan Vocational University of Science and Technology, Zhoukou, Henan 466001, China
2.
Henan Key Laboratory of Rare Earth Functional Materials, Henan International Joint Laboratory of Biomedical Nanomaterials, Zhoukou Normal University, Zhoukou, Henan 466001, China

Corresponding author: Fei LI, lifei@zknu.edu.cn
Received Date: 9 December 2024
Revised Date: 30 July 2025

Figures(10)

To investigate the photothermal toxicity of Prussian blue nanoparticles (PB NPs) on cervical cancer cells (HeLa cells) and their underlying mechanisms, uniformly sized and well-dispersed PB NPs were synthesized. Using HeLa cells as the experimental model, techniques such as MTT assay, confocal microscopy, and flow cytometry were employed to evaluate the effects of PB NPs on cell viability, membrane permeability, apoptosis, cell cycle arrest, and reactive oxygen species (ROS) generation. The MTT assay revealed that PB NPs inhibited HeLa cell proliferation in a concentration and laser power-dependent manner. At a mass concentration of 50 μg·mL^-1 and an 808 nm laser irradiation with a power density of 0.2 W·cm^-2, PB NPs reduced HeLa cell viability to 24.2%. Trypan blue staining and Calcein-AM/PI double-staining confocal microscopy demonstrated that the photothermal cytotoxicity of PB NPs primarily manifests as increased cell membrane permeability. Annexin V-FITC flow cytometry indicated that PB NPs induce apoptosis in HeLa cells. Furthermore, DCFH-DA fluorescence assays and flow cytometry showed that 20 μg·mL^-1 PB NPs under 808 nm laser irradiation (0.3 W·cm^-2) elevated intracellular ROS levels by 11.5-fold and induced cell cycle arrest at the G2/M phase. The above results suggest that the photothermal toxicity effect of PB NPs on HeLa cells is achieved by increasing cell membrane permeability, inducing cell cycle arrest, elevating ROS generation, and triggering apoptosis.
- Prussian blue,
- nanoparticles,
- photothermal toxicity
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